Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation
Main Author: | |
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Publication Date: | 2017 |
Other Authors: | , , , , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10400.15/4040 |
Summary: | Neuroinflammation is an integral part of the neurodegeneration process inherent to several aging dysfunctions. Within the central nervous system, microglia are the effective immune cells, responsible for neuroinflammatory responses. In this study, raspberries were subjected to in vitro digestion simulation to obtain the components that result from the gastrointestinal (GI) conditions, which would be bioaccessible and available for blood uptake. Both the original raspberry extract and the gastrointestinal bioaccessible (GIB) fraction protected neuronal and microglia cells against H2O2-induced oxidative stress and lipopolysaccharide (LPS)-induced inflammation, at low concentrations. Furthermore, this neuroprotective capacity was independent of intracellular ROS scavenging mechanisms. We show for the first time that raspberry metabolites present in the GIB fraction significantly inhibited microglial pro-inflammatory activation by LPS, through the inhibition of Iba1 expression, TNF-alpha release and NO production. Altogether, this study reveals that raspberry polyphenols may present a dietary route to the retardation or amelioration of neurodegenerative-related dysfunctions. (C) 2016 Elsevier Ltd. All rights reserved. |
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Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activationPolyphenolsRaspberryBioaccessibilityOxidative stressNeuroprotective mechanismsNeuroinflammationNeuroinflammation is an integral part of the neurodegeneration process inherent to several aging dysfunctions. Within the central nervous system, microglia are the effective immune cells, responsible for neuroinflammatory responses. In this study, raspberries were subjected to in vitro digestion simulation to obtain the components that result from the gastrointestinal (GI) conditions, which would be bioaccessible and available for blood uptake. Both the original raspberry extract and the gastrointestinal bioaccessible (GIB) fraction protected neuronal and microglia cells against H2O2-induced oxidative stress and lipopolysaccharide (LPS)-induced inflammation, at low concentrations. Furthermore, this neuroprotective capacity was independent of intracellular ROS scavenging mechanisms. We show for the first time that raspberry metabolites present in the GIB fraction significantly inhibited microglial pro-inflammatory activation by LPS, through the inhibition of Iba1 expression, TNF-alpha release and NO production. Altogether, this study reveals that raspberry polyphenols may present a dietary route to the retardation or amelioration of neurodegenerative-related dysfunctions. (C) 2016 Elsevier Ltd. All rights reserved.This work was supported by iNOVA4Health Research Unit (LISBOA-01-0145-FEDER-007344), which is co-funded by Fundacao para a Ciencia e Tecnologia (FCT)/Ministerio da Ciencia e do Ensino Superior, through national funds, and by Fundo Europeu de Desenvolvimento Regional FEDER (Portugal) under the PT2020 Partnership Agreement. The study was also supported by the FCT projects PTDC/SAU-FAR/118787/2010 and in part by UID/DTP/04138/2013 and the IF/01097/2013 (CNS). Moreover, the authors would also acknowledge the support of the EUBerry FP7 KBBE-2010-4 265942 (DS and CNS), BacHBerry FP7-KBBE-2013-613793 (DS, GG and CNS) and SFRH/BD/86584/2012 (IF). The authors (DS and GMD) also specially acknowledge for the support from the Scottish Government's Rural and Environment Science and Analytical Services DivisionElsevierRepositório Científico do Instituto Politécnico de SantarémGarcia, G.Nanni, S.Figueira, I.Ivanov, I.McDougall, G. J.Stewart, D.Ferreira, R. B.Pinto, PaulaSilva, R. F. M.Brites, D.Nunes Dos Santos, Cláudia2022-07-14T17:02:53Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.15/4040engGarcia, G.; Nanni, S.; Figueira, I.; Ivanov, I. McDougall, G.J.; Stewart, D., Ferreira, R.B.; Pinto, P. Silva, R.F. ; Brites, D. & Santos, C.N. (2017). Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation. Food chemistry,215: 274-2830308-8146https://doi.org/10.1016/j.foodchem.2016.07.128info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-01-21T07:36:43Zoai:repositorio.ipsantarem.pt:10400.15/4040Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T01:55:42.136435Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation |
title |
Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation |
spellingShingle |
Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation Garcia, G. Polyphenols Raspberry Bioaccessibility Oxidative stress Neuroprotective mechanisms Neuroinflammation |
title_short |
Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation |
title_full |
Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation |
title_fullStr |
Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation |
title_full_unstemmed |
Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation |
title_sort |
Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation |
author |
Garcia, G. |
author_facet |
Garcia, G. Nanni, S. Figueira, I. Ivanov, I. McDougall, G. J. Stewart, D. Ferreira, R. B. Pinto, Paula Silva, R. F. M. Brites, D. Nunes Dos Santos, Cláudia |
author_role |
author |
author2 |
Nanni, S. Figueira, I. Ivanov, I. McDougall, G. J. Stewart, D. Ferreira, R. B. Pinto, Paula Silva, R. F. M. Brites, D. Nunes Dos Santos, Cláudia |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Politécnico de Santarém |
dc.contributor.author.fl_str_mv |
Garcia, G. Nanni, S. Figueira, I. Ivanov, I. McDougall, G. J. Stewart, D. Ferreira, R. B. Pinto, Paula Silva, R. F. M. Brites, D. Nunes Dos Santos, Cláudia |
dc.subject.por.fl_str_mv |
Polyphenols Raspberry Bioaccessibility Oxidative stress Neuroprotective mechanisms Neuroinflammation |
topic |
Polyphenols Raspberry Bioaccessibility Oxidative stress Neuroprotective mechanisms Neuroinflammation |
description |
Neuroinflammation is an integral part of the neurodegeneration process inherent to several aging dysfunctions. Within the central nervous system, microglia are the effective immune cells, responsible for neuroinflammatory responses. In this study, raspberries were subjected to in vitro digestion simulation to obtain the components that result from the gastrointestinal (GI) conditions, which would be bioaccessible and available for blood uptake. Both the original raspberry extract and the gastrointestinal bioaccessible (GIB) fraction protected neuronal and microglia cells against H2O2-induced oxidative stress and lipopolysaccharide (LPS)-induced inflammation, at low concentrations. Furthermore, this neuroprotective capacity was independent of intracellular ROS scavenging mechanisms. We show for the first time that raspberry metabolites present in the GIB fraction significantly inhibited microglial pro-inflammatory activation by LPS, through the inhibition of Iba1 expression, TNF-alpha release and NO production. Altogether, this study reveals that raspberry polyphenols may present a dietary route to the retardation or amelioration of neurodegenerative-related dysfunctions. (C) 2016 Elsevier Ltd. All rights reserved. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2017-01-01T00:00:00Z 2022-07-14T17:02:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.15/4040 |
url |
http://hdl.handle.net/10400.15/4040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Garcia, G.; Nanni, S.; Figueira, I.; Ivanov, I. McDougall, G.J.; Stewart, D., Ferreira, R.B.; Pinto, P. Silva, R.F. ; Brites, D. & Santos, C.N. (2017). Bioaccessible (poly)phenol metabolites from raspberry protect neural cells from oxidative stress and attenuate microglia activation. Food chemistry,215: 274-283 0308-8146 https://doi.org/10.1016/j.foodchem.2016.07.128 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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