Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditions

Detalhes bibliográficos
Autor(a) principal: Gomes, Manuela E.
Data de Publicação: 2001
Outros Autores: Reis, R. L., Cunha, A. M., Blitterswijk, C. A. van, Bruijn, J. D. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/3907
Resumo: This work reports on the biocompatibility evaluation of new biodegradable starch-based polymers that are under consideration for use in orthopaedic temporary applications and as tissue engineering scaffolds. It has been shown in previous works that by using these polymers it is both possible to produce polymer/hydroxyapatite (HA) composites (with or without the use of coupling agents) with mechanical properties matching those of the human bone, and to obtain 3D structures generated by solid blowing agents, that are suitable for tissue engineering applications. This study was focused on establishing the influence of several additives (ceramic fillers, blowing agents and coupling agents) and processing methods/conditions on the biocompatibility of the materials described above. The cytotoxicity of the materials was evaluated using cell culture methods, according to ISO/EN 109935 guidelines. A cell suspension of human osteosarcoma cells (HOS) was also seeded on a blend of corn starch with ethylene vinyl alcohol (SEVA-C) and on SEVA-C/HA composites, in order to have a preliminary indication on cell adhesion and proliferation on the materials surface. In general, the obtained results show that all the different materials based on SEVA-C, (which are being investigated for use in several biomedical applications), as well as all the additives (including the novel coupling agents) and different processing methods required to obtain the different properties/products, can be used without inducing a cytotoxic behaviour to the developed biomaterials
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spelling Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditionsStarch-based polymers biodegradablePolymersCytotoxicityOrthopaedicsScience & TechnologyThis work reports on the biocompatibility evaluation of new biodegradable starch-based polymers that are under consideration for use in orthopaedic temporary applications and as tissue engineering scaffolds. It has been shown in previous works that by using these polymers it is both possible to produce polymer/hydroxyapatite (HA) composites (with or without the use of coupling agents) with mechanical properties matching those of the human bone, and to obtain 3D structures generated by solid blowing agents, that are suitable for tissue engineering applications. This study was focused on establishing the influence of several additives (ceramic fillers, blowing agents and coupling agents) and processing methods/conditions on the biocompatibility of the materials described above. The cytotoxicity of the materials was evaluated using cell culture methods, according to ISO/EN 109935 guidelines. A cell suspension of human osteosarcoma cells (HOS) was also seeded on a blend of corn starch with ethylene vinyl alcohol (SEVA-C) and on SEVA-C/HA composites, in order to have a preliminary indication on cell adhesion and proliferation on the materials surface. In general, the obtained results show that all the different materials based on SEVA-C, (which are being investigated for use in several biomedical applications), as well as all the additives (including the novel coupling agents) and different processing methods required to obtain the different properties/products, can be used without inducing a cytotoxic behaviour to the developed biomaterialsElsevierUniversidade do MinhoGomes, Manuela E.Reis, R. L.Cunha, A. M.Blitterswijk, C. A. vanBruijn, J. D. de20012001-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/3907eng"Biomaterials". ISSN 0142-9612. 22:13 (Jul. 2001) 1911-1917.0142-961210.1016/S0142-9612(00)00377-X11396897info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:38:42Zoai:repositorium.sdum.uminho.pt:1822/3907Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:35:13.228092Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditions
title Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditions
spellingShingle Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditions
Gomes, Manuela E.
Starch-based polymers biodegradable
Polymers
Cytotoxicity
Orthopaedics
Science & Technology
title_short Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditions
title_full Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditions
title_fullStr Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditions
title_full_unstemmed Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditions
title_sort Cytocompatibility and response of osteoblastic-like cells to starch-based polymers : effect of several additives and processing conditions
author Gomes, Manuela E.
author_facet Gomes, Manuela E.
Reis, R. L.
Cunha, A. M.
Blitterswijk, C. A. van
Bruijn, J. D. de
author_role author
author2 Reis, R. L.
Cunha, A. M.
Blitterswijk, C. A. van
Bruijn, J. D. de
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Gomes, Manuela E.
Reis, R. L.
Cunha, A. M.
Blitterswijk, C. A. van
Bruijn, J. D. de
dc.subject.por.fl_str_mv Starch-based polymers biodegradable
Polymers
Cytotoxicity
Orthopaedics
Science & Technology
topic Starch-based polymers biodegradable
Polymers
Cytotoxicity
Orthopaedics
Science & Technology
description This work reports on the biocompatibility evaluation of new biodegradable starch-based polymers that are under consideration for use in orthopaedic temporary applications and as tissue engineering scaffolds. It has been shown in previous works that by using these polymers it is both possible to produce polymer/hydroxyapatite (HA) composites (with or without the use of coupling agents) with mechanical properties matching those of the human bone, and to obtain 3D structures generated by solid blowing agents, that are suitable for tissue engineering applications. This study was focused on establishing the influence of several additives (ceramic fillers, blowing agents and coupling agents) and processing methods/conditions on the biocompatibility of the materials described above. The cytotoxicity of the materials was evaluated using cell culture methods, according to ISO/EN 109935 guidelines. A cell suspension of human osteosarcoma cells (HOS) was also seeded on a blend of corn starch with ethylene vinyl alcohol (SEVA-C) and on SEVA-C/HA composites, in order to have a preliminary indication on cell adhesion and proliferation on the materials surface. In general, the obtained results show that all the different materials based on SEVA-C, (which are being investigated for use in several biomedical applications), as well as all the additives (including the novel coupling agents) and different processing methods required to obtain the different properties/products, can be used without inducing a cytotoxic behaviour to the developed biomaterials
publishDate 2001
dc.date.none.fl_str_mv 2001
2001-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/3907
url http://hdl.handle.net/1822/3907
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv "Biomaterials". ISSN 0142-9612. 22:13 (Jul. 2001) 1911-1917.
0142-9612
10.1016/S0142-9612(00)00377-X
11396897
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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