An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium

Detalhes bibliográficos
Autor(a) principal: Azevedo, A. S.
Data de Publicação: 2017
Outros Autores: Almeida, Carina, Gomes, Luciana C., Ferreira, Carla, Mergulhão, Filipe J., Melo, Luís F., Azevedo, Nuno F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/43317
Resumo: Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.bej.2016.11.013.
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spelling An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortiumEscherichia coliDelftia tsuruhatensisuncommon bacteriaflow cellurine flowcatheter-associated urinary tract infectionsScience & TechnologySupplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.bej.2016.11.013.Uncommon bacteria, such as Delftia tusurhatensis have been isolated from CAUTIs in combination with well-established pathogenic bacteria such as Escherichia coli. Nonetheless, the reason why E. coli coexists with other bacteria instead of outcompeting and completely eliminating them is unknown. As such, a flow cell reactor simulating the hydrodynamic conditions found in CAUTIs (shear rate of 15 s1) was used to characterize the microbial physiology of E. coli and D. tsuruhatensis individually and in consortium, in terms of the growth kinetics and substrate uptake. Single-species biofilms showed that up to 48 h the cultivable cell counts significantly increased for both species (p < 0.05). When in dual-species biofilm, E. coli outnumbered D. tsuruhatensis up to 16 h and then D. tsuruhatensis gained a fitness advantage. However, the assessment of the spatial distribution of the dual-species biofilm by LNA/2OMe-FISH revealed that E. coli and D. tsuruhatensis coexist and tend to co-aggregate over time, which suggests that both bacteria are able to cooperate synergistically. Substrate uptake measurements revealed that D. tsuruhatensis metabolized citric acid more rapidly, presumably leaving more uric acid available in the medium to be used by E. coli. In conclusion, E. coli and uncommon bacteria seem to cooperate, when sharing the same environment under dynamic conditions, leading to the persistence of both bacteria in a stable microbial community.This work was financially supported by: Project POCI-01-0145-FEDER-006939 – Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE funded by FEDER funds through COMPETE2020 – Programa Operacional Competitividade e Internacionalização (POCI) – and by national funds through FCT- Fundação para a Ciência e a Tecnologia; Project “DNA mimics [PIC/IC/82815/2007]; PhD fellowships [SFRH/BD/82663/2011 and SFRH/BD/80400/2011]. The authors would like to thank M. Fenice for kindly providing the D. tsuruhatensis BM90. Finally, the authors would also like to thank Diana Vilas Boas from “Laboratório de Investigação em Biofilmes Rosário Oliveira”, at Centre of Biological Engineering, University of Minho (Braga, Portugal) for kindly helping in the edition of CLSM images.ElsevierUniversidade do MinhoAzevedo, A. S.Almeida, CarinaGomes, Luciana C.Ferreira, CarlaMergulhão, Filipe J.Melo, Luís F.Azevedo, Nuno F.2017-02-152017-02-15T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/43317engAzevedo, A. S.; Almeida, Carina; Gomes, Luciana C.; Ferreira, Carla; Mergulhão, Filipe J.; Melo, Luí­s F.; Azevedo, Nuno F., An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium. Biochemical Engineering Journal, 118, 64-69, 20171369-703X10.1016/j.bej.2016.11.013http://www.elsevier.com/locate/issn/1369703Xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:11:37Zoai:repositorium.sdum.uminho.pt:1822/43317Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:03:25.526044Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium
title An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium
spellingShingle An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium
Azevedo, A. S.
Escherichia coli
Delftia tsuruhatensis
uncommon bacteria
flow cell
urine flow
catheter-associated urinary tract infections
Science & Technology
title_short An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium
title_full An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium
title_fullStr An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium
title_full_unstemmed An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium
title_sort An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium
author Azevedo, A. S.
author_facet Azevedo, A. S.
Almeida, Carina
Gomes, Luciana C.
Ferreira, Carla
Mergulhão, Filipe J.
Melo, Luís F.
Azevedo, Nuno F.
author_role author
author2 Almeida, Carina
Gomes, Luciana C.
Ferreira, Carla
Mergulhão, Filipe J.
Melo, Luís F.
Azevedo, Nuno F.
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Azevedo, A. S.
Almeida, Carina
Gomes, Luciana C.
Ferreira, Carla
Mergulhão, Filipe J.
Melo, Luís F.
Azevedo, Nuno F.
dc.subject.por.fl_str_mv Escherichia coli
Delftia tsuruhatensis
uncommon bacteria
flow cell
urine flow
catheter-associated urinary tract infections
Science & Technology
topic Escherichia coli
Delftia tsuruhatensis
uncommon bacteria
flow cell
urine flow
catheter-associated urinary tract infections
Science & Technology
description Supplementary data associated with this article can be found, in the online version, at http://dx.doi.org/10.1016/j.bej.2016.11.013.
publishDate 2017
dc.date.none.fl_str_mv 2017-02-15
2017-02-15T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/43317
url http://hdl.handle.net/1822/43317
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Azevedo, A. S.; Almeida, Carina; Gomes, Luciana C.; Ferreira, Carla; Mergulhão, Filipe J.; Melo, Luí­s F.; Azevedo, Nuno F., An in vitro model of catheter-associated urinary tract infections to investigate the role of uncommon bacteria on the Escherichia coli microbial consortium. Biochemical Engineering Journal, 118, 64-69, 2017
1369-703X
10.1016/j.bej.2016.11.013
http://www.elsevier.com/locate/issn/1369703X
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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