Screening of novel bioactive peptides from goat casein: in silico to in vitro validation
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/36848 |
Resumo: | Food-derived bioactive peptides are of great interest to science and industry due to evolving drivers of food product innovation, including health and wellness. This study aims to draw attention through a critical study on how bioinformatics analysis is employed in the identification of bioactive peptides in the laboratory. An in silico analysis (PeptideRanker, BIOPEP, AHTpin, and mAHTPred) of a list of peptides from goat casein hydrolysate was performed to predict which sequences could potentially be bioactive. To validate the predictions, the in vitro antihypertensive potential of the five peptides with the highest potential was first measured. Then, for three of these, gastrointestinal digestion was simulated in vitro, followed by the analysis of the resulting ACE inhibitory activity as well as antioxidant capacity. We thus observed that the use of new computational biology technologies to predict peptide sequences is an important research tool, but they should not be used alone and complementarity with various in vitro and in vivo assays is essential. |
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Screening of novel bioactive peptides from goat casein: in silico to in vitro validationACE inhibitionAntihypertensive activityAntioxidant activityBioactive peptideBioinformaticsIn silico predictionORACFood-derived bioactive peptides are of great interest to science and industry due to evolving drivers of food product innovation, including health and wellness. This study aims to draw attention through a critical study on how bioinformatics analysis is employed in the identification of bioactive peptides in the laboratory. An in silico analysis (PeptideRanker, BIOPEP, AHTpin, and mAHTPred) of a list of peptides from goat casein hydrolysate was performed to predict which sequences could potentially be bioactive. To validate the predictions, the in vitro antihypertensive potential of the five peptides with the highest potential was first measured. Then, for three of these, gastrointestinal digestion was simulated in vitro, followed by the analysis of the resulting ACE inhibitory activity as well as antioxidant capacity. We thus observed that the use of new computational biology technologies to predict peptide sequences is an important research tool, but they should not be used alone and complementarity with various in vitro and in vivo assays is essential.Veritati - Repositório Institucional da Universidade Católica PortuguesaCoscueta, Ezequiel R.Batista, PatríciaGomes, José Erick GalindoSilva, Roberto daPintado, Maria Manuela2022-03-02T15:53:05Z2022-03-012022-03-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/36848eng1661-659610.3390/ijms2305243985125038672PMC891056035269581000771500300001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:42:18ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Screening of novel bioactive peptides from goat casein: in silico to in vitro validation |
title |
Screening of novel bioactive peptides from goat casein: in silico to in vitro validation |
spellingShingle |
Screening of novel bioactive peptides from goat casein: in silico to in vitro validation Coscueta, Ezequiel R. ACE inhibition Antihypertensive activity Antioxidant activity Bioactive peptide Bioinformatics In silico prediction ORAC |
title_short |
Screening of novel bioactive peptides from goat casein: in silico to in vitro validation |
title_full |
Screening of novel bioactive peptides from goat casein: in silico to in vitro validation |
title_fullStr |
Screening of novel bioactive peptides from goat casein: in silico to in vitro validation |
title_full_unstemmed |
Screening of novel bioactive peptides from goat casein: in silico to in vitro validation |
title_sort |
Screening of novel bioactive peptides from goat casein: in silico to in vitro validation |
author |
Coscueta, Ezequiel R. |
author_facet |
Coscueta, Ezequiel R. Batista, Patrícia Gomes, José Erick Galindo Silva, Roberto da Pintado, Maria Manuela |
author_role |
author |
author2 |
Batista, Patrícia Gomes, José Erick Galindo Silva, Roberto da Pintado, Maria Manuela |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Coscueta, Ezequiel R. Batista, Patrícia Gomes, José Erick Galindo Silva, Roberto da Pintado, Maria Manuela |
dc.subject.por.fl_str_mv |
ACE inhibition Antihypertensive activity Antioxidant activity Bioactive peptide Bioinformatics In silico prediction ORAC |
topic |
ACE inhibition Antihypertensive activity Antioxidant activity Bioactive peptide Bioinformatics In silico prediction ORAC |
description |
Food-derived bioactive peptides are of great interest to science and industry due to evolving drivers of food product innovation, including health and wellness. This study aims to draw attention through a critical study on how bioinformatics analysis is employed in the identification of bioactive peptides in the laboratory. An in silico analysis (PeptideRanker, BIOPEP, AHTpin, and mAHTPred) of a list of peptides from goat casein hydrolysate was performed to predict which sequences could potentially be bioactive. To validate the predictions, the in vitro antihypertensive potential of the five peptides with the highest potential was first measured. Then, for three of these, gastrointestinal digestion was simulated in vitro, followed by the analysis of the resulting ACE inhibitory activity as well as antioxidant capacity. We thus observed that the use of new computational biology technologies to predict peptide sequences is an important research tool, but they should not be used alone and complementarity with various in vitro and in vivo assays is essential. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-03-02T15:53:05Z 2022-03-01 2022-03-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/36848 |
url |
http://hdl.handle.net/10400.14/36848 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
1661-6596 10.3390/ijms23052439 85125038672 PMC8910560 35269581 000771500300001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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1777303216121184256 |