Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms
Main Author: | |
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Publication Date: | 2014 |
Other Authors: | , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/31643 |
Summary: | The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50 μg L−1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic. |
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Influence of tetracycline on the microbial community composition and activity of nitrifying biofilmsAntibioticsBacterial communityNitrifying biofilmsTetracycline removalResistance genesScience & TechnologyThe present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50 μg L−1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic.The work described in this paper was financially supported by FCT - Foundation for Science and Technology (Portugal) - by the PhD student grant SFRH/BD/44596/2008.Pergamon Press Ltd.Universidade do MinhoMatos, M. I.Pereira, M. A.Parpot, PierBrito, A. G.Nogueira, R.20142014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/31643engMatos, Maria; Pereira, M. A.; Parpot, Pier; Brito, A. G.; Nogueira, R., Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms. Chemosphere, 117, 295-302, 20140045-65350045-653510.1016/j.chemosphere.2014.06.09425127228info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:53:12Zoai:repositorium.sdum.uminho.pt:1822/31643Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:52:31.948693Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms |
title |
Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms |
spellingShingle |
Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms Matos, M. I. Antibiotics Bacterial community Nitrifying biofilms Tetracycline removal Resistance genes Science & Technology |
title_short |
Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms |
title_full |
Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms |
title_fullStr |
Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms |
title_full_unstemmed |
Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms |
title_sort |
Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms |
author |
Matos, M. I. |
author_facet |
Matos, M. I. Pereira, M. A. Parpot, Pier Brito, A. G. Nogueira, R. |
author_role |
author |
author2 |
Pereira, M. A. Parpot, Pier Brito, A. G. Nogueira, R. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Matos, M. I. Pereira, M. A. Parpot, Pier Brito, A. G. Nogueira, R. |
dc.subject.por.fl_str_mv |
Antibiotics Bacterial community Nitrifying biofilms Tetracycline removal Resistance genes Science & Technology |
topic |
Antibiotics Bacterial community Nitrifying biofilms Tetracycline removal Resistance genes Science & Technology |
description |
The present work aims to evaluate the bacterial composition and activity (carbon and nitrogen removal) of nitrifying biofilms exposed to 50 μg L−1 of tetracycline. The tetracycline removal efficiency and the occurrence of tetracycline resistance (tet) genes were also studied. Two sequencing batch biofilm reactors (SBBRs) fed with synthetic wastewater were operated without (SBBR1) and with (SBBR2) the antibiotic. Both SBBRs showed similar organic matter biodegradation and nitrification activity. Tetracycline removal was about 28% and biodegradation was probably the principal removal mechanism of the antibiotic. Polymerase chain reaction-denaturing gradient gel electrophoresis analysis of the bacterial community showed shifts leading to not only the fading of some ribotypes, but also the emergence of new ones in the biofilm with tetracycline. The study of the tet genes showed that tet(S) was only detected in the biofilm with tetracycline, suggesting a relationship between its occurrence and the presence of the antibiotic. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/31643 |
url |
http://hdl.handle.net/1822/31643 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Matos, Maria; Pereira, M. A.; Parpot, Pier; Brito, A. G.; Nogueira, R., Influence of tetracycline on the microbial community composition and activity of nitrifying biofilms. Chemosphere, 117, 295-302, 2014 0045-6535 0045-6535 10.1016/j.chemosphere.2014.06.094 25127228 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Pergamon Press Ltd. |
publisher.none.fl_str_mv |
Pergamon Press Ltd. |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799133117964877824 |