ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed samples
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/64695 |
Resumo: | Lead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLEIA), based on the mAb we generated, were developed for the detection of lead (II). In total, 50% inhibitory concentrations (IC<sub>50</sub>) of lead (II) were 9.4 ng/mL (ELISA) and 1.4 ng/mL (CLEIA); the limits of detection (LOD) were 0.7 ng/mL (ic-ELISA) and 0.1 ng/mL (ic-CLEIA), respectively. Cross-reactivities of the mAb toward other metal ions were less than 0.943%, indicating that the obtained mAb has high sensitivity and specificity. The recovery rates were 82.1%–108.3% (ic-ELISA) and 80.1%–98.8% (ic-CLEIA), respectively. The developed methods are feasible for the determination of trace lead (II) in various samples with high sensitivity, specificity, fastness, simplicity and accuracy. |
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ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed sampleslead (II)ELISAmonoclonal antibody (mAb)isothiocyanobenzyl-EDTA (ITCBE)chemiluminescent enzyme immunoassay (CLEIA)Science & TechnologyLead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLEIA), based on the mAb we generated, were developed for the detection of lead (II). In total, 50% inhibitory concentrations (IC<sub>50</sub>) of lead (II) were 9.4 ng/mL (ELISA) and 1.4 ng/mL (CLEIA); the limits of detection (LOD) were 0.7 ng/mL (ic-ELISA) and 0.1 ng/mL (ic-CLEIA), respectively. Cross-reactivities of the mAb toward other metal ions were less than 0.943%, indicating that the obtained mAb has high sensitivity and specificity. The recovery rates were 82.1%–108.3% (ic-ELISA) and 80.1%–98.8% (ic-CLEIA), respectively. The developed methods are feasible for the determination of trace lead (II) in various samples with high sensitivity, specificity, fastness, simplicity and accuracy.This research was funded by National Natural Science Foundation of China, grant numbers 31572556, 31873006; the Key Program for International S&T Cooperation Project of Shaanxi Province, grant number 2017KW-ZD-10; and the Incubation Project on State Key Laboratory of Biological Resources and Ecological Environment of Qinba Areas, grant number SLGPT2019KF04-04.Multidisciplinary Digital Publishing InstituteUniversidade do MinhoXu, LongSuo, Xiao-yiZhang, QiLi, Xin-pingChen, ChenZhang, Xiao-ying2020-03-082020-03-08T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/64695engXu, L.; Suo, X.-Y.; Zhang, Q.; Li, X.-P.; Chen, C.; Zhang, X.-Y. ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples. Foods 2020, 9, 305.2304-815810.3390/foods9030305https://www.mdpi.com/2304-8158/9/3/305info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:24:56Zoai:repositorium.sdum.uminho.pt:1822/64695Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:19:03.801603Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed samples |
title |
ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed samples |
spellingShingle |
ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed samples Xu, Long lead (II) ELISA monoclonal antibody (mAb) isothiocyanobenzyl-EDTA (ITCBE) chemiluminescent enzyme immunoassay (CLEIA) Science & Technology |
title_short |
ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed samples |
title_full |
ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed samples |
title_fullStr |
ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed samples |
title_full_unstemmed |
ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed samples |
title_sort |
ELISA and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead (II) in water, food and feed samples |
author |
Xu, Long |
author_facet |
Xu, Long Suo, Xiao-yi Zhang, Qi Li, Xin-ping Chen, Chen Zhang, Xiao-ying |
author_role |
author |
author2 |
Suo, Xiao-yi Zhang, Qi Li, Xin-ping Chen, Chen Zhang, Xiao-ying |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Xu, Long Suo, Xiao-yi Zhang, Qi Li, Xin-ping Chen, Chen Zhang, Xiao-ying |
dc.subject.por.fl_str_mv |
lead (II) ELISA monoclonal antibody (mAb) isothiocyanobenzyl-EDTA (ITCBE) chemiluminescent enzyme immunoassay (CLEIA) Science & Technology |
topic |
lead (II) ELISA monoclonal antibody (mAb) isothiocyanobenzyl-EDTA (ITCBE) chemiluminescent enzyme immunoassay (CLEIA) Science & Technology |
description |
Lead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLEIA), based on the mAb we generated, were developed for the detection of lead (II). In total, 50% inhibitory concentrations (IC<sub>50</sub>) of lead (II) were 9.4 ng/mL (ELISA) and 1.4 ng/mL (CLEIA); the limits of detection (LOD) were 0.7 ng/mL (ic-ELISA) and 0.1 ng/mL (ic-CLEIA), respectively. Cross-reactivities of the mAb toward other metal ions were less than 0.943%, indicating that the obtained mAb has high sensitivity and specificity. The recovery rates were 82.1%–108.3% (ic-ELISA) and 80.1%–98.8% (ic-CLEIA), respectively. The developed methods are feasible for the determination of trace lead (II) in various samples with high sensitivity, specificity, fastness, simplicity and accuracy. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-03-08 2020-03-08T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/64695 |
url |
http://hdl.handle.net/1822/64695 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Xu, L.; Suo, X.-Y.; Zhang, Q.; Li, X.-P.; Chen, C.; Zhang, X.-Y. ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples. Foods 2020, 9, 305. 2304-8158 10.3390/foods9030305 https://www.mdpi.com/2304-8158/9/3/305 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
publisher.none.fl_str_mv |
Multidisciplinary Digital Publishing Institute |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
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1799132647298957312 |