Establishment of a genomic DNA extraction protocol for bamboo species

• Main Author: Silva,Rudieli M.
• Publication Date: 2018
• Other Authors: Ribeiro,Nathalia P.
• Format: Article
• Language: eng
• Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Download full: http://scielo.pt/scielo.php?script=sci_arttext&pid=S0871-018X2018000300026
• topic: DNA extraction; Guadua angustifolia; molecular markers; polymerase chain reaction

The bamboos Bambusa vulgaris and Guadua angustifolia are considered the most important species in South America. Generally, bamboo taxonomy is conducted by analysis of plants morphology, which sometimes may present morphological similarities, complicating the achievement of precise results. This fact can be changed by application of molecular biology methods; however, the Brazilian research of bamboo in molecular level is still limited. The Brazilian state of Mato Grosso do Sul has fragments of a natural bamboo forest with external characteristics similar to Guadua. In order to provide information about bamboo species, this paper evaluates the application of a modified protocol for DNA extraction from young leaves of two accesses of Guadua cultivated in Campo Grande/MS. It also evaluates the amplification efficiency of the matK and rbcL regions of the DNA isolated. It was possible to extract a mean of 487.5 ng μL-1 of DNA for both species with a purity level of 1.91. The molecular marker rbcL amplified the regions of 300 bp for both species, while the marker matK amplified the regions of 850 bp, also for both accesses. Results show that the modified protocol is useful for bamboo’s DNA extraction, with high concentration, quality, and able to be amplified by PCR.