Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck)
Main Author: | |
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Publication Date: | 2000 |
Other Authors: | , |
Format: | Conference object |
Language: | eng |
Source: | Repositório Institucional da UNESP |
Download full: | http://www.actahort.org/books/535/535_4.htm http://hdl.handle.net/11449/66372 |
Summary: | Sweet orange is considered a very important species in the citrus world market and presents wide morphological variability. However, its characterization at the molecular level by random amplified polymorphic DNA (RAPD) and isozyme markers is not appropriate. Microsatellite or simple sequence repeats (SSRs) have been suggested as ideal for studies in cultures of vegetative propagation and as value markers for mapping in several species. However, information on microsatellite polymorphism in citrus species is scarce. In this work, microsatellite markers (AG-repeats) were developed from an enrichment library of genomic DNA of sweet orange cv. Pera (Citrus sinensis [L.] Osbeck), and 31 cultivars of sweet orange were evaluated. Evaluation of 18 microsatellite primers did not permit differentiation of the varieties studied. New microsatellite primers are being evaluated with the aim of detecting polymorphisms among the cultivars and closely related species to be used in genetic mapping programs. |
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Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck)BreedingMolecular markersPolymerase chain reactionPolymorphismSweet orange is considered a very important species in the citrus world market and presents wide morphological variability. However, its characterization at the molecular level by random amplified polymorphic DNA (RAPD) and isozyme markers is not appropriate. Microsatellite or simple sequence repeats (SSRs) have been suggested as ideal for studies in cultures of vegetative propagation and as value markers for mapping in several species. However, information on microsatellite polymorphism in citrus species is scarce. In this work, microsatellite markers (AG-repeats) were developed from an enrichment library of genomic DNA of sweet orange cv. Pera (Citrus sinensis [L.] Osbeck), and 31 cultivars of sweet orange were evaluated. Evaluation of 18 microsatellite primers did not permit differentiation of the varieties studied. New microsatellite primers are being evaluated with the aim of detecting polymorphisms among the cultivars and closely related species to be used in genetic mapping programs.Dep. de Genética IB/UNESP, Distrito de Rubiao Júnior, CEP 18.600.000 - Botucatu/SPCentro de Citricultura Sylvio Moreira Institute Agronômico de Campinas, Via Anhanguera Km 158, CP 04, Cordeirópolis SP, 13490-970Dep. de Genética IB/UNESP, Distrito de Rubiao Júnior, CEP 18.600.000 - Botucatu/SPUniversidade Estadual Paulista (Unesp)Institute Agronômico de CampinasNovelli, V. M. [UNESP]Cristofani, M.Machado, M. A.2014-05-27T11:20:11Z2014-05-27T11:20:11Z2000-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/conferenceObject47-50http://www.actahort.org/books/535/535_4.htmActa Horticulturae, v. 535, p. 47-50.0567-7572http://hdl.handle.net/11449/66372WOS:0001664007000042-s2.0-0037646174Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengActa Horticulturae0,198info:eu-repo/semantics/openAccess2021-10-23T21:41:42Zoai:repositorio.unesp.br:11449/66372Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T21:41:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck) |
title |
Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck) |
spellingShingle |
Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck) Novelli, V. M. [UNESP] Breeding Molecular markers Polymerase chain reaction Polymorphism |
title_short |
Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck) |
title_full |
Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck) |
title_fullStr |
Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck) |
title_full_unstemmed |
Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck) |
title_sort |
Evaluation of microsatellite markers in cultivars of sweet orange (Citrus sinensis [L.] Osbeck) |
author |
Novelli, V. M. [UNESP] |
author_facet |
Novelli, V. M. [UNESP] Cristofani, M. Machado, M. A. |
author_role |
author |
author2 |
Cristofani, M. Machado, M. A. |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Institute Agronômico de Campinas |
dc.contributor.author.fl_str_mv |
Novelli, V. M. [UNESP] Cristofani, M. Machado, M. A. |
dc.subject.por.fl_str_mv |
Breeding Molecular markers Polymerase chain reaction Polymorphism |
topic |
Breeding Molecular markers Polymerase chain reaction Polymorphism |
description |
Sweet orange is considered a very important species in the citrus world market and presents wide morphological variability. However, its characterization at the molecular level by random amplified polymorphic DNA (RAPD) and isozyme markers is not appropriate. Microsatellite or simple sequence repeats (SSRs) have been suggested as ideal for studies in cultures of vegetative propagation and as value markers for mapping in several species. However, information on microsatellite polymorphism in citrus species is scarce. In this work, microsatellite markers (AG-repeats) were developed from an enrichment library of genomic DNA of sweet orange cv. Pera (Citrus sinensis [L.] Osbeck), and 31 cultivars of sweet orange were evaluated. Evaluation of 18 microsatellite primers did not permit differentiation of the varieties studied. New microsatellite primers are being evaluated with the aim of detecting polymorphisms among the cultivars and closely related species to be used in genetic mapping programs. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-12-01 2014-05-27T11:20:11Z 2014-05-27T11:20:11Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/conferenceObject |
format |
conferenceObject |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.actahort.org/books/535/535_4.htm Acta Horticulturae, v. 535, p. 47-50. 0567-7572 http://hdl.handle.net/11449/66372 WOS:000166400700004 2-s2.0-0037646174 |
url |
http://www.actahort.org/books/535/535_4.htm http://hdl.handle.net/11449/66372 |
identifier_str_mv |
Acta Horticulturae, v. 535, p. 47-50. 0567-7572 WOS:000166400700004 2-s2.0-0037646174 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Acta Horticulturae 0,198 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
47-50 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789407751700480 |