Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/58258 |
Resumo: | Metastasis remains the most common cause of death in cancer patients. Inhibition of metalloproteinases (MMPs) is an interesting approach to cancer therapy because of their role in the degradation of extracellular matrix (ECM), cell-cell, and cell-ECM interactions, modulating key events in cell migration and invasion. Herein, we show the cytotoxic and antimetastatic effects of the third fraction (FR3) from Bauhinia variegata candida (Bvc) stem on human cervical tumor cells (HeLa) and human peripheral blood mononuclear cells (PBMCs). FR3 inhibited MMP-2 and MMP-9 activity, indicated by zymogram. This fraction was cytotoxic to HeLa cells and noncytotoxic to PBMCs and decreased HeLa cell migration and invasion. FR3 is believed to stimulate extrinsic apoptosis together with necroptosis, assessed by western blotting. FR3 inhibited MMP-2 activity in the HeLa supernatant, differently from the control. The atomic mass spectrometry (ESI-MS) characterization suggested the presence of glucopyranosides, D-pinitol, fatty acids, and phenolic acid. These findings provide insight suggesting that FR3 contains components with potential tumor-selective cytotoxic action in addition to the action on the migration of tumor cells, which may be due to inhibition of MMPs. |
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Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In VitroApoptosisBauhiniaCaspase 3Cell DeathCell Line, TumorCell MovementCytotoxinsFatty AcidsHeLa CellsHumansHydroxybenzoatesInositolLeukocytes, MononuclearMatrix Metalloproteinase 2Matrix Metalloproteinase 9Neoplasm InvasivenessPlant ExtractsReceptor-Interacting Protein Serine-Threonine KinasesReceptors, Tumor Necrosis Factor, Type ISignal TransductionCiências Médicas::Medicina BásicaScience & TechnologyMetastasis remains the most common cause of death in cancer patients. Inhibition of metalloproteinases (MMPs) is an interesting approach to cancer therapy because of their role in the degradation of extracellular matrix (ECM), cell-cell, and cell-ECM interactions, modulating key events in cell migration and invasion. Herein, we show the cytotoxic and antimetastatic effects of the third fraction (FR3) from Bauhinia variegata candida (Bvc) stem on human cervical tumor cells (HeLa) and human peripheral blood mononuclear cells (PBMCs). FR3 inhibited MMP-2 and MMP-9 activity, indicated by zymogram. This fraction was cytotoxic to HeLa cells and noncytotoxic to PBMCs and decreased HeLa cell migration and invasion. FR3 is believed to stimulate extrinsic apoptosis together with necroptosis, assessed by western blotting. FR3 inhibited MMP-2 activity in the HeLa supernatant, differently from the control. The atomic mass spectrometry (ESI-MS) characterization suggested the presence of glucopyranosides, D-pinitol, fatty acids, and phenolic acid. These findings provide insight suggesting that FR3 contains components with potential tumor-selective cytotoxic action in addition to the action on the migration of tumor cells, which may be due to inhibition of MMPs.Fundação de Amparo à Pesquisa de Minas Gerais (FAPEMIG), Fundação CAPES, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPQ), and FINEP (MCTI/FINEP/MS/SCTIE/DECIT-01/2013-FPXII-BIOPLAT)info:eu-repo/semantics/publishedVersionHindawi Publishing CorporationUniversidade do MinhoSantos, K. M.Gomes, I. N. F.Silva-Oliveira, R. J.Pinto, F. E.Oliveira, B. G.Chagas, R. C. R.Romão, W.Reis, R. M.Ribeiro, R. I. M. A.20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/58258engSantos, K. M., Gomes, I. N. F., Silva-Oliveira, R. J., Pinto, F. E., Oliveira, B. G., Chagas, R. C. R., ... & Ribeiro, R. I. M. A. (2018). Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro. BioMed research international, 20182314-61332314-614110.1155/2018/470248129770331https://www.hindawi.com/journals/bmri/2018/4702481/abs/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:54:37Zoai:repositorium.sdum.uminho.pt:1822/58258Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:44:05.995811Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro |
title |
Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro |
spellingShingle |
Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro Santos, K. M. Apoptosis Bauhinia Caspase 3 Cell Death Cell Line, Tumor Cell Movement Cytotoxins Fatty Acids HeLa Cells Humans Hydroxybenzoates Inositol Leukocytes, Mononuclear Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Neoplasm Invasiveness Plant Extracts Receptor-Interacting Protein Serine-Threonine Kinases Receptors, Tumor Necrosis Factor, Type I Signal Transduction Ciências Médicas::Medicina Básica Science & Technology |
title_short |
Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro |
title_full |
Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro |
title_fullStr |
Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro |
title_full_unstemmed |
Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro |
title_sort |
Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro |
author |
Santos, K. M. |
author_facet |
Santos, K. M. Gomes, I. N. F. Silva-Oliveira, R. J. Pinto, F. E. Oliveira, B. G. Chagas, R. C. R. Romão, W. Reis, R. M. Ribeiro, R. I. M. A. |
author_role |
author |
author2 |
Gomes, I. N. F. Silva-Oliveira, R. J. Pinto, F. E. Oliveira, B. G. Chagas, R. C. R. Romão, W. Reis, R. M. Ribeiro, R. I. M. A. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Santos, K. M. Gomes, I. N. F. Silva-Oliveira, R. J. Pinto, F. E. Oliveira, B. G. Chagas, R. C. R. Romão, W. Reis, R. M. Ribeiro, R. I. M. A. |
dc.subject.por.fl_str_mv |
Apoptosis Bauhinia Caspase 3 Cell Death Cell Line, Tumor Cell Movement Cytotoxins Fatty Acids HeLa Cells Humans Hydroxybenzoates Inositol Leukocytes, Mononuclear Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Neoplasm Invasiveness Plant Extracts Receptor-Interacting Protein Serine-Threonine Kinases Receptors, Tumor Necrosis Factor, Type I Signal Transduction Ciências Médicas::Medicina Básica Science & Technology |
topic |
Apoptosis Bauhinia Caspase 3 Cell Death Cell Line, Tumor Cell Movement Cytotoxins Fatty Acids HeLa Cells Humans Hydroxybenzoates Inositol Leukocytes, Mononuclear Matrix Metalloproteinase 2 Matrix Metalloproteinase 9 Neoplasm Invasiveness Plant Extracts Receptor-Interacting Protein Serine-Threonine Kinases Receptors, Tumor Necrosis Factor, Type I Signal Transduction Ciências Médicas::Medicina Básica Science & Technology |
description |
Metastasis remains the most common cause of death in cancer patients. Inhibition of metalloproteinases (MMPs) is an interesting approach to cancer therapy because of their role in the degradation of extracellular matrix (ECM), cell-cell, and cell-ECM interactions, modulating key events in cell migration and invasion. Herein, we show the cytotoxic and antimetastatic effects of the third fraction (FR3) from Bauhinia variegata candida (Bvc) stem on human cervical tumor cells (HeLa) and human peripheral blood mononuclear cells (PBMCs). FR3 inhibited MMP-2 and MMP-9 activity, indicated by zymogram. This fraction was cytotoxic to HeLa cells and noncytotoxic to PBMCs and decreased HeLa cell migration and invasion. FR3 is believed to stimulate extrinsic apoptosis together with necroptosis, assessed by western blotting. FR3 inhibited MMP-2 activity in the HeLa supernatant, differently from the control. The atomic mass spectrometry (ESI-MS) characterization suggested the presence of glucopyranosides, D-pinitol, fatty acids, and phenolic acid. These findings provide insight suggesting that FR3 contains components with potential tumor-selective cytotoxic action in addition to the action on the migration of tumor cells, which may be due to inhibition of MMPs. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/58258 |
url |
http://hdl.handle.net/1822/58258 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Santos, K. M., Gomes, I. N. F., Silva-Oliveira, R. J., Pinto, F. E., Oliveira, B. G., Chagas, R. C. R., ... & Ribeiro, R. I. M. A. (2018). Bauhinia variegata candida Fraction Induces Tumor Cell Death by Activation of Caspase-3, RIP, and TNF-R1 and Inhibits Cell Migration and Invasion In Vitro. BioMed research international, 2018 2314-6133 2314-6141 10.1155/2018/4702481 29770331 https://www.hindawi.com/journals/bmri/2018/4702481/abs/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132189521084416 |