In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests

Detalhes bibliográficos
Autor(a) principal: Costa, Carla
Data de Publicação: 2015
Outros Autores: Brandão, Fátima, Bessa, Maria João, Costa, Solange, Valdiglesias, Vanessa, Kiliç, Gözde, Fernández-Bertólez, Natalia, Quaresma, Pedro, Pereira, Eulália, Pásaro, Eduardo, Laffon, Blanca, Teixeira, João Paulo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.18/3350
Resumo: Superparamagnetic iron oxide nanoparticles (ION) have attracted great interest for use in several biomedical fields. In general, they are considered biocompatible, but little is known of their effects on the human nervous system. The main objective of this work was to evaluate the cytotoxicity of two ION (magnetite), coated with silica and oleic acid, previously determining the possible interference of the ION with the methodological procedures to assure the reliability of the results obtained. Human neuroblastoma SHSY5Y and glioblastoma A172 cells were exposed to different concentrations of ION (5-300 µg ml(-1) ), prepared in complete and serum-free cell culture medium for three exposure times (3, 6 and 24 h). Cytotoxicity was evaluated by means of the MTT, neutral red uptake and alamar blue assays. Characterization of the main physical-chemical properties of the ION tested was also performed. Results demonstrated that both ION could significantly alter absorbance readings. To reduce these interferences, protocols were modified by introducing additional washing steps and cell-free systems. Significant decreases in cell viability were observed for both cell lines in specific conditions by all assays. In general, oleic acid-coated ION were less cytotoxic than silica-coated ION; besides, a serum-protective effect was observed for both ION studied and cell lines. These results contribute to increase the knowledge of the potential harmful effects of ION on the human nervous system. Understanding these effects is essential to establish satisfactory regulatory policies on the safe use of magnetite nanoparticles in biomedical applications.
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spelling In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability testsIron Oxide NanoparticlesToxicologyCytotoxicityGlioblastoma CellsMagnetiteNeuroblastoma CellsOleic Acid-coated Iron Oxide NanoparticlesSilica-coated Iron Oxide NanoparticlesGenotoxicidade AmbientalSuperparamagnetic iron oxide nanoparticles (ION) have attracted great interest for use in several biomedical fields. In general, they are considered biocompatible, but little is known of their effects on the human nervous system. The main objective of this work was to evaluate the cytotoxicity of two ION (magnetite), coated with silica and oleic acid, previously determining the possible interference of the ION with the methodological procedures to assure the reliability of the results obtained. Human neuroblastoma SHSY5Y and glioblastoma A172 cells were exposed to different concentrations of ION (5-300 µg ml(-1) ), prepared in complete and serum-free cell culture medium for three exposure times (3, 6 and 24 h). Cytotoxicity was evaluated by means of the MTT, neutral red uptake and alamar blue assays. Characterization of the main physical-chemical properties of the ION tested was also performed. Results demonstrated that both ION could significantly alter absorbance readings. To reduce these interferences, protocols were modified by introducing additional washing steps and cell-free systems. Significant decreases in cell viability were observed for both cell lines in specific conditions by all assays. In general, oleic acid-coated ION were less cytotoxic than silica-coated ION; besides, a serum-protective effect was observed for both ION studied and cell lines. These results contribute to increase the knowledge of the potential harmful effects of ION on the human nervous system. Understanding these effects is essential to establish satisfactory regulatory policies on the safe use of magnetite nanoparticles in biomedical applications.This work was funded by Xunta de Galicia (EM 2012/079) and by TD1204 MODENA COST Action. G.K. was supported by a fellowship from the University of A Coruña.John Wiley & Sons, Ltd.Repositório Científico do Instituto Nacional de SaúdeCosta, CarlaBrandão, FátimaBessa, Maria JoãoCosta, SolangeValdiglesias, VanessaKiliç, GözdeFernández-Bertólez, NataliaQuaresma, PedroPereira, EuláliaPásaro, EduardoLaffon, BlancaTeixeira, João Paulo2016-02-16T17:48:10Z2015-07-262015-07-26T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3350engJ Appl Toxicol. 2016 Mar;36(3):361-72. doi: 10.1002/jat.3213. Epub 2015 Jul 26.0260-437X10.1002/jat.3213info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:46Zoai:repositorio.insa.pt:10400.18/3350Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:16.931723Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
title In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
spellingShingle In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
Costa, Carla
Iron Oxide Nanoparticles
Toxicology
Cytotoxicity
Glioblastoma Cells
Magnetite
Neuroblastoma Cells
Oleic Acid-coated Iron Oxide Nanoparticles
Silica-coated Iron Oxide Nanoparticles
Genotoxicidade Ambiental
title_short In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
title_full In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
title_fullStr In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
title_full_unstemmed In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
title_sort In vitro cytotoxicity of superparamagnetic iron oxide nanoparticles on neuronal and glial cells. Evaluation of nanoparticle interference with viability tests
author Costa, Carla
author_facet Costa, Carla
Brandão, Fátima
Bessa, Maria João
Costa, Solange
Valdiglesias, Vanessa
Kiliç, Gözde
Fernández-Bertólez, Natalia
Quaresma, Pedro
Pereira, Eulália
Pásaro, Eduardo
Laffon, Blanca
Teixeira, João Paulo
author_role author
author2 Brandão, Fátima
Bessa, Maria João
Costa, Solange
Valdiglesias, Vanessa
Kiliç, Gözde
Fernández-Bertólez, Natalia
Quaresma, Pedro
Pereira, Eulália
Pásaro, Eduardo
Laffon, Blanca
Teixeira, João Paulo
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório Científico do Instituto Nacional de Saúde
dc.contributor.author.fl_str_mv Costa, Carla
Brandão, Fátima
Bessa, Maria João
Costa, Solange
Valdiglesias, Vanessa
Kiliç, Gözde
Fernández-Bertólez, Natalia
Quaresma, Pedro
Pereira, Eulália
Pásaro, Eduardo
Laffon, Blanca
Teixeira, João Paulo
dc.subject.por.fl_str_mv Iron Oxide Nanoparticles
Toxicology
Cytotoxicity
Glioblastoma Cells
Magnetite
Neuroblastoma Cells
Oleic Acid-coated Iron Oxide Nanoparticles
Silica-coated Iron Oxide Nanoparticles
Genotoxicidade Ambiental
topic Iron Oxide Nanoparticles
Toxicology
Cytotoxicity
Glioblastoma Cells
Magnetite
Neuroblastoma Cells
Oleic Acid-coated Iron Oxide Nanoparticles
Silica-coated Iron Oxide Nanoparticles
Genotoxicidade Ambiental
description Superparamagnetic iron oxide nanoparticles (ION) have attracted great interest for use in several biomedical fields. In general, they are considered biocompatible, but little is known of their effects on the human nervous system. The main objective of this work was to evaluate the cytotoxicity of two ION (magnetite), coated with silica and oleic acid, previously determining the possible interference of the ION with the methodological procedures to assure the reliability of the results obtained. Human neuroblastoma SHSY5Y and glioblastoma A172 cells were exposed to different concentrations of ION (5-300 µg ml(-1) ), prepared in complete and serum-free cell culture medium for three exposure times (3, 6 and 24 h). Cytotoxicity was evaluated by means of the MTT, neutral red uptake and alamar blue assays. Characterization of the main physical-chemical properties of the ION tested was also performed. Results demonstrated that both ION could significantly alter absorbance readings. To reduce these interferences, protocols were modified by introducing additional washing steps and cell-free systems. Significant decreases in cell viability were observed for both cell lines in specific conditions by all assays. In general, oleic acid-coated ION were less cytotoxic than silica-coated ION; besides, a serum-protective effect was observed for both ION studied and cell lines. These results contribute to increase the knowledge of the potential harmful effects of ION on the human nervous system. Understanding these effects is essential to establish satisfactory regulatory policies on the safe use of magnetite nanoparticles in biomedical applications.
publishDate 2015
dc.date.none.fl_str_mv 2015-07-26
2015-07-26T00:00:00Z
2016-02-16T17:48:10Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.18/3350
url http://hdl.handle.net/10400.18/3350
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv J Appl Toxicol. 2016 Mar;36(3):361-72. doi: 10.1002/jat.3213. Epub 2015 Jul 26.
0260-437X
10.1002/jat.3213
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv John Wiley & Sons, Ltd.
publisher.none.fl_str_mv John Wiley & Sons, Ltd.
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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