In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.18/3349 |
Resumo: | Iron oxide nanoparticles (ION) have been widely used in biomedical applications, for both diagnosis and therapy, due to their unique magnetic properties. They are intensively explored in neuromedicine mostly because of their ability to cross the blood brain barrier. Hence, their potential harmful effects on neuronal cells need to be carefully assessed. The objective of this study was to evaluate the toxicity of silica-coated ION (S-ION) (10–200 μg ml−1) on human neuronal SHSY5Y cells. Alterations in the cell cycle, cell death by apoptosis or necrosis, and membrane integrity were assessed as cytotoxicity parameters. Genotoxicity was determined by a γH2AX assay, a micronucleus (MN) test, and a comet assay. Complementarily, possible effects on DNA damage repair were also analysed by means of a DNA repair competence assay. All analyses were performed in complete and serum-free cell culture media. Iron ion release from the nanoparticles was notable only in complete medium. Despite being effectively internalized by the neuronal cells, S-ION presented in general low cytotoxicity; positive results were only obtained in some assays at the highest concentrations and/or the longest exposure time tested (24 h). Genotoxicity evaluations in serum-free medium were negative for all conditions assayed; in complete medium, dose and time-dependent increase in DNA damage not related to the production of double strand breaks or chromosome loss (according to the results of the γH2AX assay and MN test), was obtained. The presence of serum slightly influenced the behaviour of S-ION; further studies to investigate the formation of a protein corona and its role in nanoparticle toxicity are necessary. |
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In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cellsIron Oxide NanoparticlesGenotoxicityCytotoxicityDNA EepairSHSY5Y cellsGenotoxicidade AmbientalIron oxide nanoparticles (ION) have been widely used in biomedical applications, for both diagnosis and therapy, due to their unique magnetic properties. They are intensively explored in neuromedicine mostly because of their ability to cross the blood brain barrier. Hence, their potential harmful effects on neuronal cells need to be carefully assessed. The objective of this study was to evaluate the toxicity of silica-coated ION (S-ION) (10–200 μg ml−1) on human neuronal SHSY5Y cells. Alterations in the cell cycle, cell death by apoptosis or necrosis, and membrane integrity were assessed as cytotoxicity parameters. Genotoxicity was determined by a γH2AX assay, a micronucleus (MN) test, and a comet assay. Complementarily, possible effects on DNA damage repair were also analysed by means of a DNA repair competence assay. All analyses were performed in complete and serum-free cell culture media. Iron ion release from the nanoparticles was notable only in complete medium. Despite being effectively internalized by the neuronal cells, S-ION presented in general low cytotoxicity; positive results were only obtained in some assays at the highest concentrations and/or the longest exposure time tested (24 h). Genotoxicity evaluations in serum-free medium were negative for all conditions assayed; in complete medium, dose and time-dependent increase in DNA damage not related to the production of double strand breaks or chromosome loss (according to the results of the γH2AX assay and MN test), was obtained. The presence of serum slightly influenced the behaviour of S-ION; further studies to investigate the formation of a protein corona and its role in nanoparticle toxicity are necessary.This work was funded by Xunta de Galicia (EM 2012/079) and by TD1204 MODENA COST Action. G. Kiliç was supported by a fellowship from the University of A Coruña.Royal Society of ChemistryRepositório Científico do Instituto Nacional de SaúdeKiliç, GözdeCosta, CarlaFernández-Bertólez, NataliaPásaro, EduardoTeixeira, João PauloLaffon, BlancaValdiglesias, Vanessa2016-02-16T17:42:14Z2015-10-232015-10-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.18/3349engToxicol. Res. 2016; 5: 235-47. doi:10.1039/C5TX00206K. Epub 2015 Oct 232045-453810.1039/C5TX00206Kinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-20T15:39:46Zoai:repositorio.insa.pt:10400.18/3349Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:38:16.867145Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells |
title |
In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells |
spellingShingle |
In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells Kiliç, Gözde Iron Oxide Nanoparticles Genotoxicity Cytotoxicity DNA Eepair SHSY5Y cells Genotoxicidade Ambiental |
title_short |
In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells |
title_full |
In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells |
title_fullStr |
In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells |
title_full_unstemmed |
In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells |
title_sort |
In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells |
author |
Kiliç, Gözde |
author_facet |
Kiliç, Gözde Costa, Carla Fernández-Bertólez, Natalia Pásaro, Eduardo Teixeira, João Paulo Laffon, Blanca Valdiglesias, Vanessa |
author_role |
author |
author2 |
Costa, Carla Fernández-Bertólez, Natalia Pásaro, Eduardo Teixeira, João Paulo Laffon, Blanca Valdiglesias, Vanessa |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório Científico do Instituto Nacional de Saúde |
dc.contributor.author.fl_str_mv |
Kiliç, Gözde Costa, Carla Fernández-Bertólez, Natalia Pásaro, Eduardo Teixeira, João Paulo Laffon, Blanca Valdiglesias, Vanessa |
dc.subject.por.fl_str_mv |
Iron Oxide Nanoparticles Genotoxicity Cytotoxicity DNA Eepair SHSY5Y cells Genotoxicidade Ambiental |
topic |
Iron Oxide Nanoparticles Genotoxicity Cytotoxicity DNA Eepair SHSY5Y cells Genotoxicidade Ambiental |
description |
Iron oxide nanoparticles (ION) have been widely used in biomedical applications, for both diagnosis and therapy, due to their unique magnetic properties. They are intensively explored in neuromedicine mostly because of their ability to cross the blood brain barrier. Hence, their potential harmful effects on neuronal cells need to be carefully assessed. The objective of this study was to evaluate the toxicity of silica-coated ION (S-ION) (10–200 μg ml−1) on human neuronal SHSY5Y cells. Alterations in the cell cycle, cell death by apoptosis or necrosis, and membrane integrity were assessed as cytotoxicity parameters. Genotoxicity was determined by a γH2AX assay, a micronucleus (MN) test, and a comet assay. Complementarily, possible effects on DNA damage repair were also analysed by means of a DNA repair competence assay. All analyses were performed in complete and serum-free cell culture media. Iron ion release from the nanoparticles was notable only in complete medium. Despite being effectively internalized by the neuronal cells, S-ION presented in general low cytotoxicity; positive results were only obtained in some assays at the highest concentrations and/or the longest exposure time tested (24 h). Genotoxicity evaluations in serum-free medium were negative for all conditions assayed; in complete medium, dose and time-dependent increase in DNA damage not related to the production of double strand breaks or chromosome loss (according to the results of the γH2AX assay and MN test), was obtained. The presence of serum slightly influenced the behaviour of S-ION; further studies to investigate the formation of a protein corona and its role in nanoparticle toxicity are necessary. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-10-23 2015-10-23T00:00:00Z 2016-02-16T17:42:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.18/3349 |
url |
http://hdl.handle.net/10400.18/3349 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicol. Res. 2016; 5: 235-47. doi:10.1039/C5TX00206K. Epub 2015 Oct 23 2045-4538 10.1039/C5TX00206K |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
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application/pdf |
dc.publisher.none.fl_str_mv |
Royal Society of Chemistry |
publisher.none.fl_str_mv |
Royal Society of Chemistry |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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