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Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans

Detalhes bibliográficos
Autor(a) principal: Kumar J.G.S.P.
Data de Publicação: 2018
Outros Autores: Gomathi A., Gothandam K.M., Vasconcelos V.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: https://hdl.handle.net/10216/120473
Resumo: Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics. © 2018 by the authors.
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spelling Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicansActinobacteria extractantibiotic agentantifungal agentnonribosomal peptide synthetasepolyketide synthasepolyketide synthase type Ipolyketide synthase type IIRNA 16Sunclassified drugantifungal agentRNA 16SActinobacteriaAndaman and Nicobar Islandsantifungal activityArticlebacterial strainbacterium identificationbacterium isolateCandida albicanscontrolled studydrug isolationdrug screeningdrug synthesisecosystemgene sequencegenetic screeningmangrovemass fragmentographymetabolitenonhumannucleotide sequencephylogenyRNA analysissedimentStreptomycesActinobacteriaCandida albicanschemistrydrug effectgeneticsIndiamicrobial sensitivity testmicrobiologyRhizophoraceaeActinobacteriaAntifungal AgentsCandida albicansGas Chromatography-Mass SpectrometryGeologic SedimentsIndiaMicrobial Sensitivity TestsRhizophoraceaeRNA, Ribosomal, 16SStreptomycesActinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics. © 2018 by the authors.MDPI20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10216/120473eng1660339710.3390/md16020060Kumar J.G.S.P.Gomathi A.Gothandam K.M.Vasconcelos V.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-11-29T13:50:21Zoai:repositorio-aberto.up.pt:10216/120473Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T23:48:57.925525Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans
title Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans
spellingShingle Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans
Kumar J.G.S.P.
Actinobacteria extract
antibiotic agent
antifungal agent
nonribosomal peptide synthetase
polyketide synthase
polyketide synthase type I
polyketide synthase type II
RNA 16S
unclassified drug
antifungal agent
RNA 16S
Actinobacteria
Andaman and Nicobar Islands
antifungal activity
Article
bacterial strain
bacterium identification
bacterium isolate
Candida albicans
controlled study
drug isolation
drug screening
drug synthesis
ecosystem
gene sequence
genetic screening
mangrove
mass fragmentography
metabolite
nonhuman
nucleotide sequence
phylogeny
RNA analysis
sediment
Streptomyces
Actinobacteria
Candida albicans
chemistry
drug effect
genetics
India
microbial sensitivity test
microbiology
Rhizophoraceae
Actinobacteria
Antifungal Agents
Candida albicans
Gas Chromatography-Mass Spectrometry
Geologic Sediments
India
Microbial Sensitivity Tests
Rhizophoraceae
RNA, Ribosomal, 16S
Streptomyces
title_short Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans
title_full Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans
title_fullStr Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans
title_full_unstemmed Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans
title_sort Bioactivity assessment of indian origin-mangrove actinobacteria against candida albicans
author Kumar J.G.S.P.
author_facet Kumar J.G.S.P.
Gomathi A.
Gothandam K.M.
Vasconcelos V.
author_role author
author2 Gomathi A.
Gothandam K.M.
Vasconcelos V.
author2_role author
author
author
dc.contributor.author.fl_str_mv Kumar J.G.S.P.
Gomathi A.
Gothandam K.M.
Vasconcelos V.
dc.subject.por.fl_str_mv Actinobacteria extract
antibiotic agent
antifungal agent
nonribosomal peptide synthetase
polyketide synthase
polyketide synthase type I
polyketide synthase type II
RNA 16S
unclassified drug
antifungal agent
RNA 16S
Actinobacteria
Andaman and Nicobar Islands
antifungal activity
Article
bacterial strain
bacterium identification
bacterium isolate
Candida albicans
controlled study
drug isolation
drug screening
drug synthesis
ecosystem
gene sequence
genetic screening
mangrove
mass fragmentography
metabolite
nonhuman
nucleotide sequence
phylogeny
RNA analysis
sediment
Streptomyces
Actinobacteria
Candida albicans
chemistry
drug effect
genetics
India
microbial sensitivity test
microbiology
Rhizophoraceae
Actinobacteria
Antifungal Agents
Candida albicans
Gas Chromatography-Mass Spectrometry
Geologic Sediments
India
Microbial Sensitivity Tests
Rhizophoraceae
RNA, Ribosomal, 16S
Streptomyces
topic Actinobacteria extract
antibiotic agent
antifungal agent
nonribosomal peptide synthetase
polyketide synthase
polyketide synthase type I
polyketide synthase type II
RNA 16S
unclassified drug
antifungal agent
RNA 16S
Actinobacteria
Andaman and Nicobar Islands
antifungal activity
Article
bacterial strain
bacterium identification
bacterium isolate
Candida albicans
controlled study
drug isolation
drug screening
drug synthesis
ecosystem
gene sequence
genetic screening
mangrove
mass fragmentography
metabolite
nonhuman
nucleotide sequence
phylogeny
RNA analysis
sediment
Streptomyces
Actinobacteria
Candida albicans
chemistry
drug effect
genetics
India
microbial sensitivity test
microbiology
Rhizophoraceae
Actinobacteria
Antifungal Agents
Candida albicans
Gas Chromatography-Mass Spectrometry
Geologic Sediments
India
Microbial Sensitivity Tests
Rhizophoraceae
RNA, Ribosomal, 16S
Streptomyces
description Actinobacteria is found to have a potent metabolic activity against pathogens. The present study reveals the assessment of potent antifungal secondary metabolites from actinobacteria isolated from Indian marine mangrove sediments. The samples were collected from the coastal regions of Muthupet, Andaman and the Nicobar Islands. Identification was carried out using 16S rRNA analysis and biosynthetic genes (Polyketide synthase type I/II and Non-ribosomal peptide synthase) were screened. Actinobacteria were assayed for their antifungal activity against 16 clinical Candida albicans and the compound analysis was performed using gas chromatography-mass spectrometry GC-MS. The 31 actinobacterial strains were isolated and 16S rRNA gene sequencing revealed that this ecosystem is rich on actinobacteria, with Streptomyces as the predominant genus. The PCR based screening of biosynthetic genes revealed the presence of PKS-I in six strains, PKS-II in four strains and NRPS in 11 strains. The isolated actinobacteria VITGAP240 and VITGAP241 (two isolates) were found to have a potential antifungal activity against all the tested C. albicans. GC-MS results revealed that the actinobacterial compounds were belonging to heterocyclic, polyketides and peptides. Overall, the strains possess a wide spectrum of antifungal properties which affords the production of significant bioactive metabolites as potential antibiotics. © 2018 by the authors.
publishDate 2018
dc.date.none.fl_str_mv 2018
2018-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://hdl.handle.net/10216/120473
url https://hdl.handle.net/10216/120473
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 16603397
10.3390/md16020060
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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