Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritis

Detalhes bibliográficos
Autor(a) principal: Perpétuo, Inês Pedro
Data de Publicação: 2017
Outros Autores: Caetano-Lopes, Joana, Rodrigues, Ana Maria, Campanilho-Marques, Raquel, Ponte, Cristina, Canhao, Helena, Ainola, Mari, Fonseca, João Eurico
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10451/51417
Resumo: Copyright © 2017 Inês P. Perpétuo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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spelling Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritisCopyright © 2017 Inês P. Perpétuo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.Objective. Tumor necrosis factor (TNF) increases circulating osteoclast (OC) precursors numbers by promoting their proliferation and differentiation. The aim of this study was to assess the effect of TNF inhibitors (TNFi) on the differentiation and activity of OC in rheumatoid arthritis (RA) patients. Methods. Seventeen RA patients treated with TNFi were analyzed at baseline and after a minimum follow-up period of 6 months. Blood samples were collected to assess receptor activator of nuclear factor kappa-B ligand (RANKL) surface expression on circulating leukocytes and frequency and phenotype of monocyte subpopulations. Quantification of serum levels of bone turnover markers, in vitro OC differentiation assays, and qRT-PCR for OC specific genes was performed. Results. After TNFi therapy, patients had reduced RANKL surface expression in B-lymphocytes and the frequency of circulating classical CD14brightCD16- monocytes was decreased. Serum levels of sRANKL, sRANKL/OPG ratio, and CTX-I were reduced in RA patients after TNFi treatment. Moreover, after exposure to TNFi, osteoclast differentiation and activity were decreased, as well as the expression of TRAF6 and cathepsin K. Conclusion. We propose that TNFi arrests bone loss and erosion, through two pathways: direct reduction of osteoclast precursor numbers and inhibition of intracellular signaling pathways acting through TRAF6.This work was supported by Fundação para a Ciência e Tecnologia (SFRH/BD/70533/2010 to Inês P. Perpétuo) and by a research grant from Investigator-Initiated Studies Program of Merck Sharp & Dohme Corp. (Merck_P08574 to João E. Fonseca).HindawiRepositório da Universidade de LisboaPerpétuo, Inês PedroCaetano-Lopes, JoanaRodrigues, Ana MariaCampanilho-Marques, RaquelPonte, CristinaCanhao, HelenaAinola, MariFonseca, João Eurico2022-02-18T15:40:46Z20172017-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10451/51417engBiomed Res Int. 2017;2017:26904022314-613310.1155/2017/26904022314-6141info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-14T15:38:20ZPortal AgregadorONG
dc.title.none.fl_str_mv Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritis
title Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritis
spellingShingle Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritis
Perpétuo, Inês Pedro
title_short Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritis
title_full Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritis
title_fullStr Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritis
title_full_unstemmed Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritis
title_sort Effect of tumor necrosis factor inhibitor therapy on osteoclasts precursors in rheumatoid arthritis
author Perpétuo, Inês Pedro
author_facet Perpétuo, Inês Pedro
Caetano-Lopes, Joana
Rodrigues, Ana Maria
Campanilho-Marques, Raquel
Ponte, Cristina
Canhao, Helena
Ainola, Mari
Fonseca, João Eurico
author_role author
author2 Caetano-Lopes, Joana
Rodrigues, Ana Maria
Campanilho-Marques, Raquel
Ponte, Cristina
Canhao, Helena
Ainola, Mari
Fonseca, João Eurico
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Repositório da Universidade de Lisboa
dc.contributor.author.fl_str_mv Perpétuo, Inês Pedro
Caetano-Lopes, Joana
Rodrigues, Ana Maria
Campanilho-Marques, Raquel
Ponte, Cristina
Canhao, Helena
Ainola, Mari
Fonseca, João Eurico
description Copyright © 2017 Inês P. Perpétuo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-01-01T00:00:00Z
2022-02-18T15:40:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10451/51417
url http://hdl.handle.net/10451/51417
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biomed Res Int. 2017;2017:2690402
2314-6133
10.1155/2017/2690402
2314-6141
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hindawi
publisher.none.fl_str_mv Hindawi
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
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