Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | https://doi.org/10.1155/2017/2690402 |
Resumo: | This work was supported by Fundacao, para a Ciencia e Tecnologia (SFRH/BD/70533/2010 to Ines P. Perpetuo) and by a research grant from Investigator-Initiated Studies Program of Merck Sharp & Dohme Corp. (Merck-P08574 to Joao E. Fonseca). The authors would like to thank Biobanco-IMM, Lisbon Academic Medical Center, Lisbon, Portugal, for samples collection and storage. The authors would also like to thank Monica Medina and Soraia Silva for their valuable help in image analysis |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritisadultantagonists and inhibitorsArthritis, Rheumatoidbiosynthesisfemalefollow uphumanmalemetabolismmiddle agedmonocytemonocyte macrophage precursor cellosteoclastpathologyImmunology and Microbiology(all)Biochemistry, Genetics and Molecular Biology(all)This work was supported by Fundacao, para a Ciencia e Tecnologia (SFRH/BD/70533/2010 to Ines P. Perpetuo) and by a research grant from Investigator-Initiated Studies Program of Merck Sharp & Dohme Corp. (Merck-P08574 to Joao E. Fonseca). The authors would like to thank Biobanco-IMM, Lisbon Academic Medical Center, Lisbon, Portugal, for samples collection and storage. The authors would also like to thank Monica Medina and Soraia Silva for their valuable help in image analysisObjective. Tumor necrosis factor (TNF) increases circulating osteoclast (OC) precursors numbers by promoting their proliferation and differentiation. The aim of this study was to assess the effect of TNF inhibitors (TNFi) on the differentiation and activity of OC in rheumatoid arthritis (RA) patients. Methods. Seventeen RA patients treated with TNFi were analyzed at baseline and after a minimum follow-up period of 6 months. Blood samples were collected to assess receptor activator of nuclear factor kappa-B ligand (RANKL) surface expression on circulating leukocytes and frequency and phenotype of monocyte subpopulations. Quantification of serum levels of bone turnover markers, in vitro OC differentiation assays, and qRT-PCR for OC specific genes was performed. Results. After TNFi therapy, patients had reduced RANKL surface expression in B-lymphocytes and the frequency of circulating classical C D 14 b r i g h t CD16- monocytes was decreased. Serum levels of sRANKL, sRANKL/OPG ratio, and CTX-I were reduced in RA patients after TNFi treatment. Moreover, after exposure to TNFi, osteoclast differentiation and activity were decreased, as well as the expression of TRAF6 and cathepsin K. Conclusion. We propose that TNFi arrests bone loss and erosion, through two pathways: direct reduction of osteoclast precursor numbers and inhibition of intracellular signaling pathways acting through TRAF6.NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM)Centro de Estudos de Doenças Crónicas (CEDOC)RUNPerpétuo, Inês P.Caetano-Lopes, JoanaRodrigues, Ana MariaCampanilho-Marques, RaquelPonte, CristinaCanhão, HelenaAinola, MariFonseca, João E.2017-09-11T22:02:11Z2017-02-132017-02-13T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttps://doi.org/10.1155/2017/2690402eng2314-6133PURE: 3096372http://www.scopus.com/inward/record.url?scp=85014258589&partnerID=8YFLogxKhttps://doi.org/10.1155/2017/2690402info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-10T15:40:14ZPortal AgregadorONG |
| dc.title.none.fl_str_mv |
Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis |
| title |
Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis |
| spellingShingle |
Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis Perpétuo, Inês P. adult antagonists and inhibitors Arthritis, Rheumatoid biosynthesis female follow up human male metabolism middle aged monocyte monocyte macrophage precursor cell osteoclast pathology Immunology and Microbiology(all) Biochemistry, Genetics and Molecular Biology(all) |
| title_short |
Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis |
| title_full |
Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis |
| title_fullStr |
Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis |
| title_full_unstemmed |
Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis |
| title_sort |
Effect of Tumor Necrosis Factor Inhibitor Therapy on Osteoclasts Precursors in Rheumatoid Arthritis |
| author |
Perpétuo, Inês P. |
| author_facet |
Perpétuo, Inês P. Caetano-Lopes, Joana Rodrigues, Ana Maria Campanilho-Marques, Raquel Ponte, Cristina Canhão, Helena Ainola, Mari Fonseca, João E. |
| author_role |
author |
| author2 |
Caetano-Lopes, Joana Rodrigues, Ana Maria Campanilho-Marques, Raquel Ponte, Cristina Canhão, Helena Ainola, Mari Fonseca, João E. |
| author2_role |
author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA Medical School|Faculdade de Ciências Médicas (NMS|FCM) Centro de Estudos de Doenças Crónicas (CEDOC) RUN |
| dc.contributor.author.fl_str_mv |
Perpétuo, Inês P. Caetano-Lopes, Joana Rodrigues, Ana Maria Campanilho-Marques, Raquel Ponte, Cristina Canhão, Helena Ainola, Mari Fonseca, João E. |
| dc.subject.por.fl_str_mv |
adult antagonists and inhibitors Arthritis, Rheumatoid biosynthesis female follow up human male metabolism middle aged monocyte monocyte macrophage precursor cell osteoclast pathology Immunology and Microbiology(all) Biochemistry, Genetics and Molecular Biology(all) |
| topic |
adult antagonists and inhibitors Arthritis, Rheumatoid biosynthesis female follow up human male metabolism middle aged monocyte monocyte macrophage precursor cell osteoclast pathology Immunology and Microbiology(all) Biochemistry, Genetics and Molecular Biology(all) |
| description |
This work was supported by Fundacao, para a Ciencia e Tecnologia (SFRH/BD/70533/2010 to Ines P. Perpetuo) and by a research grant from Investigator-Initiated Studies Program of Merck Sharp & Dohme Corp. (Merck-P08574 to Joao E. Fonseca). The authors would like to thank Biobanco-IMM, Lisbon Academic Medical Center, Lisbon, Portugal, for samples collection and storage. The authors would also like to thank Monica Medina and Soraia Silva for their valuable help in image analysis |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-09-11T22:02:11Z 2017-02-13 2017-02-13T00:00:00Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.uri.fl_str_mv |
https://doi.org/10.1155/2017/2690402 |
| url |
https://doi.org/10.1155/2017/2690402 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2314-6133 PURE: 3096372 http://www.scopus.com/inward/record.url?scp=85014258589&partnerID=8YFLogxK https://doi.org/10.1155/2017/2690402 |
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info:eu-repo/semantics/openAccess |
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openAccess |
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application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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