Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.9/2519 |
Resumo: | The functional food market is growing rapidly and membrane processing offers several advantages over conventional methods for separation, fractionation and recovery of bioactive components. The aim of the present studywas to select a process that could be implemented easily on an industrial scale for the isolation of natural lactose-derived oligosaccharides (OS) from caprine whey, enabling the development of functional foods for clinical and infant nutrition. The most efficient process was the combination of a pre-treatment to eliminate proteins and fat, using an ultrafiltration (UF) membrane of 25-kDa molecular weight cutoff (MWCO), followed by a tighter UF membrane with 1-kDaMWCO. circa 90% of the carbohydrates recovered in the final retentate were OS. Capillary electrophoresis was used to evaluate the OS profile in this retentate. The combined membrane-processing system is thus a promising technique for obtaining natural concentrated OS from whey. |
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Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresisOligosaccharidesCaprine milkUltrafiltrationMembrane separationCapillary ElectrophoresisThe functional food market is growing rapidly and membrane processing offers several advantages over conventional methods for separation, fractionation and recovery of bioactive components. The aim of the present studywas to select a process that could be implemented easily on an industrial scale for the isolation of natural lactose-derived oligosaccharides (OS) from caprine whey, enabling the development of functional foods for clinical and infant nutrition. The most efficient process was the combination of a pre-treatment to eliminate proteins and fat, using an ultrafiltration (UF) membrane of 25-kDa molecular weight cutoff (MWCO), followed by a tighter UF membrane with 1-kDaMWCO. circa 90% of the carbohydrates recovered in the final retentate were OS. Capillary electrophoresis was used to evaluate the OS profile in this retentate. The combined membrane-processing system is thus a promising technique for obtaining natural concentrated OS from whey.SpringerRepositório do LNEGOliveira, DianaWilbey, R. AndrewGrandison, A. S.Roseiro, Luisa B.2014-08-11T16:16:08Z2014-01-01T00:00:00Z2014-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.9/2519engOliveira, D.L.; Wilbey, R.A.; Grandison, A.S.; Roseiro, L. Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis. In: Food and Bioprocess Technology, 2014, Vol. 7, nº 3, p. 915-9201935-5130info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-06T12:27:35Zoai:repositorio.lneg.pt:10400.9/2519Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:35:24.353979Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis |
title |
Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis |
spellingShingle |
Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis Oliveira, Diana Oligosaccharides Caprine milk Ultrafiltration Membrane separation Capillary Electrophoresis |
title_short |
Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis |
title_full |
Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis |
title_fullStr |
Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis |
title_full_unstemmed |
Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis |
title_sort |
Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis |
author |
Oliveira, Diana |
author_facet |
Oliveira, Diana Wilbey, R. Andrew Grandison, A. S. Roseiro, Luisa B. |
author_role |
author |
author2 |
Wilbey, R. Andrew Grandison, A. S. Roseiro, Luisa B. |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Repositório do LNEG |
dc.contributor.author.fl_str_mv |
Oliveira, Diana Wilbey, R. Andrew Grandison, A. S. Roseiro, Luisa B. |
dc.subject.por.fl_str_mv |
Oligosaccharides Caprine milk Ultrafiltration Membrane separation Capillary Electrophoresis |
topic |
Oligosaccharides Caprine milk Ultrafiltration Membrane separation Capillary Electrophoresis |
description |
The functional food market is growing rapidly and membrane processing offers several advantages over conventional methods for separation, fractionation and recovery of bioactive components. The aim of the present studywas to select a process that could be implemented easily on an industrial scale for the isolation of natural lactose-derived oligosaccharides (OS) from caprine whey, enabling the development of functional foods for clinical and infant nutrition. The most efficient process was the combination of a pre-treatment to eliminate proteins and fat, using an ultrafiltration (UF) membrane of 25-kDa molecular weight cutoff (MWCO), followed by a tighter UF membrane with 1-kDaMWCO. circa 90% of the carbohydrates recovered in the final retentate were OS. Capillary electrophoresis was used to evaluate the OS profile in this retentate. The combined membrane-processing system is thus a promising technique for obtaining natural concentrated OS from whey. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-08-11T16:16:08Z 2014-01-01T00:00:00Z 2014-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.9/2519 |
url |
http://hdl.handle.net/10400.9/2519 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Oliveira, D.L.; Wilbey, R.A.; Grandison, A.S.; Roseiro, L. Natural caprine whey oligosaccharides separated by membrane technology and profile evaluation by capillary electrophoresis. In: Food and Bioprocess Technology, 2014, Vol. 7, nº 3, p. 915-920 1935-5130 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Springer |
publisher.none.fl_str_mv |
Springer |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799130219627413504 |