Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/181501 |
Resumo: | The present work describes the development of a capillary electrophoresis (CE) method for the chiral discrimination of amlodipine (AML) enantiomers using cyclodextrine (CD) derivatives as chiral selectors. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector; and carboximethyl-β-CD (CM-β-CD) was selected for the enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors were varied at the same time to optimize the separation method. The optimized method (25 mM phosphate buffer, pH = 9.0, 15 mM CM-β-CD, 15 °C, + 25 kV, 30 mbar/1 second, detection wavelength 230 nm) was successfully applied for the baseline separation of AML enantiomers within 5 minutes. Successful validation and application of the proposed CE method suggest its routine use in enantioselective control of AML in pharmaceutical preparations. |
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Brazilian Journal of Pharmaceutical Sciences |
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Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresisAmlodipineCapillary electrophoresisChiral separationCyclodextrineExperimental designThe present work describes the development of a capillary electrophoresis (CE) method for the chiral discrimination of amlodipine (AML) enantiomers using cyclodextrine (CD) derivatives as chiral selectors. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector; and carboximethyl-β-CD (CM-β-CD) was selected for the enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors were varied at the same time to optimize the separation method. The optimized method (25 mM phosphate buffer, pH = 9.0, 15 mM CM-β-CD, 15 °C, + 25 kV, 30 mbar/1 second, detection wavelength 230 nm) was successfully applied for the baseline separation of AML enantiomers within 5 minutes. Successful validation and application of the proposed CE method suggest its routine use in enantioselective control of AML in pharmaceutical preparations.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2020-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/18150110.1590/s2175-97902019000318259Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18259Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18259Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e182592175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/181501/168485Copyright (c) 2020 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCârcu-Dobrin, Melania Sabău, Alexandra Georgiana Hancu, Gabriel Árpád, Gyéresi Rusu , Aura Kelemen, Hajnal Papp, Lajos Attila Cârje, Anca 2021-06-12T19:46:54Zoai:revistas.usp.br:article/181501Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2021-06-12T19:46:54Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis |
title |
Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis |
spellingShingle |
Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis Cârcu-Dobrin, Melania Amlodipine Capillary electrophoresis Chiral separation Cyclodextrine Experimental design |
title_short |
Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis |
title_full |
Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis |
title_fullStr |
Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis |
title_full_unstemmed |
Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis |
title_sort |
Chiral discrimination of amlodipine from pharmaceutical products using capillary electrophoresis |
author |
Cârcu-Dobrin, Melania |
author_facet |
Cârcu-Dobrin, Melania Sabău, Alexandra Georgiana Hancu, Gabriel Árpád, Gyéresi Rusu , Aura Kelemen, Hajnal Papp, Lajos Attila Cârje, Anca |
author_role |
author |
author2 |
Sabău, Alexandra Georgiana Hancu, Gabriel Árpád, Gyéresi Rusu , Aura Kelemen, Hajnal Papp, Lajos Attila Cârje, Anca |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Cârcu-Dobrin, Melania Sabău, Alexandra Georgiana Hancu, Gabriel Árpád, Gyéresi Rusu , Aura Kelemen, Hajnal Papp, Lajos Attila Cârje, Anca |
dc.subject.por.fl_str_mv |
Amlodipine Capillary electrophoresis Chiral separation Cyclodextrine Experimental design |
topic |
Amlodipine Capillary electrophoresis Chiral separation Cyclodextrine Experimental design |
description |
The present work describes the development of a capillary electrophoresis (CE) method for the chiral discrimination of amlodipine (AML) enantiomers using cyclodextrine (CD) derivatives as chiral selectors. A large number of native and derivatized, neutral and ionized CD derivatives were screened to find the optimal chiral selector; and carboximethyl-β-CD (CM-β-CD) was selected for the enantiomeric discrimination. A factorial analysis study was performed by orthogonal experimental design in which several factors were varied at the same time to optimize the separation method. The optimized method (25 mM phosphate buffer, pH = 9.0, 15 mM CM-β-CD, 15 °C, + 25 kV, 30 mbar/1 second, detection wavelength 230 nm) was successfully applied for the baseline separation of AML enantiomers within 5 minutes. Successful validation and application of the proposed CE method suggest its routine use in enantioselective control of AML in pharmaceutical preparations. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/181501 10.1590/s2175-97902019000318259 |
url |
https://www.revistas.usp.br/bjps/article/view/181501 |
identifier_str_mv |
10.1590/s2175-97902019000318259 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/181501/168485 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18259 Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18259 Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18259 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222915080224768 |