Methods to Study Centrosomes and Cilia in Drosophila
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.7/832 |
Resumo: | The deposited item is a book chapter and is part of the series " Methods in Molecular Biology book series ([MIMB, volume 1454]) published by the publisher Humana Press. |
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Methods to Study Centrosomes and Cilia in DrosophilaDrosophilaCentrosomeBasal bodyCiliaIntraflagellar transportSensory neuronSpermThe deposited item is a book chapter and is part of the series " Methods in Molecular Biology book series ([MIMB, volume 1454]) published by the publisher Humana Press.The deposited book chapter is a pre-print version and hasn't been submitted to peer reviewing.There is no public supplementary material available for this publication.This publication hasn't any creative commons license associated.Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.Fundação Portuguesa para a Ciência e Tecnologia grants: (SFRH/BPD/87479/2012, SFRH/BD/52176/2013); EMBO installation grant; ERC starting grant.Humana PressARCAJana, Swadhin ChandraMendonça, SusanaWerner, SaschaBettencourt-Dias, Monica2018-02-07T17:16:59Z2016-08-122016-08-12T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.7/832engJana S.C., Mendonça S., Werner S., Bettencourt-Dias M. (2016) Methods to Study Centrosomes and Cilia in Drosophila. In: Satir P., Christensen S. (eds) Cilia. Methods in Molecular Biology, vol 1454. Humana Press, New York, NY10.1007/978-1-4939-3789-9_14info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-11-29T14:35:14Zoai:arca.igc.gulbenkian.pt:10400.7/832Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T16:12:03.131687Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Methods to Study Centrosomes and Cilia in Drosophila |
title |
Methods to Study Centrosomes and Cilia in Drosophila |
spellingShingle |
Methods to Study Centrosomes and Cilia in Drosophila Jana, Swadhin Chandra Drosophila Centrosome Basal body Cilia Intraflagellar transport Sensory neuron Sperm |
title_short |
Methods to Study Centrosomes and Cilia in Drosophila |
title_full |
Methods to Study Centrosomes and Cilia in Drosophila |
title_fullStr |
Methods to Study Centrosomes and Cilia in Drosophila |
title_full_unstemmed |
Methods to Study Centrosomes and Cilia in Drosophila |
title_sort |
Methods to Study Centrosomes and Cilia in Drosophila |
author |
Jana, Swadhin Chandra |
author_facet |
Jana, Swadhin Chandra Mendonça, Susana Werner, Sascha Bettencourt-Dias, Monica |
author_role |
author |
author2 |
Mendonça, Susana Werner, Sascha Bettencourt-Dias, Monica |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
ARCA |
dc.contributor.author.fl_str_mv |
Jana, Swadhin Chandra Mendonça, Susana Werner, Sascha Bettencourt-Dias, Monica |
dc.subject.por.fl_str_mv |
Drosophila Centrosome Basal body Cilia Intraflagellar transport Sensory neuron Sperm |
topic |
Drosophila Centrosome Basal body Cilia Intraflagellar transport Sensory neuron Sperm |
description |
The deposited item is a book chapter and is part of the series " Methods in Molecular Biology book series ([MIMB, volume 1454]) published by the publisher Humana Press. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-08-12 2016-08-12T00:00:00Z 2018-02-07T17:16:59Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.7/832 |
url |
http://hdl.handle.net/10400.7/832 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Jana S.C., Mendonça S., Werner S., Bettencourt-Dias M. (2016) Methods to Study Centrosomes and Cilia in Drosophila. In: Satir P., Christensen S. (eds) Cilia. Methods in Molecular Biology, vol 1454. Humana Press, New York, NY 10.1007/978-1-4939-3789-9_14 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Humana Press |
publisher.none.fl_str_mv |
Humana Press |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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