Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em Portugal
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.10/651 |
Resumo: | The differential diagnosis of Mycobacterium bovis is important in the control of transmission to the human population and also for treatment since M. bovis is naturally resistant to pyrazinamide. Eleven clinical isolates from the Fernando Fonseca Hospital with Spoligotypes indicative of M. bovis, through the absence of spacers 39-43 but that also counted with the absence of spacer 38, were analyzed. For the identification of these strains, the phenotypic analysis of pyrazinamide resistance and study of the polymorphisms of the pncA and gyrB genes were carried out. The study of the pncA polymorphism revealed that the strains analyzed did not contain the M. bovis specific mutation. In relation the gyrB polymorphisms, using the GenoTypeO MTBC kit, the strains were identified as belonging to the group M. tuberculosis, M. africanum subtipo II e M. canetti. The present investigation enabled us to define new genotypes on which future bacteriological studies should be based. Amongst these the study of the pncA polymorphism was considered important due to the immediate practical implications for the clinician. Evaluating transmission and defining groups of risk is an objective for which support from the veterinary services is considered relevant. |
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Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em PortugalSpoligotyping and pncA polymorphism: a two step scenario for Mycobacterium bovis diagnosis in Portugal.TuberculosePolimorfismo genéticoMycobacterium bovisPortugalThe differential diagnosis of Mycobacterium bovis is important in the control of transmission to the human population and also for treatment since M. bovis is naturally resistant to pyrazinamide. Eleven clinical isolates from the Fernando Fonseca Hospital with Spoligotypes indicative of M. bovis, through the absence of spacers 39-43 but that also counted with the absence of spacer 38, were analyzed. For the identification of these strains, the phenotypic analysis of pyrazinamide resistance and study of the polymorphisms of the pncA and gyrB genes were carried out. The study of the pncA polymorphism revealed that the strains analyzed did not contain the M. bovis specific mutation. In relation the gyrB polymorphisms, using the GenoTypeO MTBC kit, the strains were identified as belonging to the group M. tuberculosis, M. africanum subtipo II e M. canetti. The present investigation enabled us to define new genotypes on which future bacteriological studies should be based. Amongst these the study of the pncA polymorphism was considered important due to the immediate practical implications for the clinician. Evaluating transmission and defining groups of risk is an objective for which support from the veterinary services is considered relevant.Sociedade Portuguesa de PneumologiaRepositório do Hospital Prof. Doutor Fernando FonsecaDavid, SPortugal, CAntunes, ACalado, ACardoso, ABarros, VSancho, LSousa, JG2012-08-10T14:50:26Z2005-01-01T00:00:00Z2005-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.10/651porRev Port Pneumol. 2005 Nov-Dec;11(6):533-560873-2159info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2022-09-20T15:51:32Zoai:repositorio.hff.min-saude.pt:10400.10/651Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T15:51:53.544613Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em Portugal Spoligotyping and pncA polymorphism: a two step scenario for Mycobacterium bovis diagnosis in Portugal. |
title |
Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em Portugal |
spellingShingle |
Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em Portugal David, S Tuberculose Polimorfismo genético Mycobacterium bovis Portugal |
title_short |
Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em Portugal |
title_full |
Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em Portugal |
title_fullStr |
Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em Portugal |
title_full_unstemmed |
Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em Portugal |
title_sort |
Spoligtyping e polimorfismo do gene pncA: um cenário em duas etapas para o diagnóstico de mycobacterium bovis em Portugal |
author |
David, S |
author_facet |
David, S Portugal, C Antunes, A Calado, A Cardoso, A Barros, V Sancho, L Sousa, JG |
author_role |
author |
author2 |
Portugal, C Antunes, A Calado, A Cardoso, A Barros, V Sancho, L Sousa, JG |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Repositório do Hospital Prof. Doutor Fernando Fonseca |
dc.contributor.author.fl_str_mv |
David, S Portugal, C Antunes, A Calado, A Cardoso, A Barros, V Sancho, L Sousa, JG |
dc.subject.por.fl_str_mv |
Tuberculose Polimorfismo genético Mycobacterium bovis Portugal |
topic |
Tuberculose Polimorfismo genético Mycobacterium bovis Portugal |
description |
The differential diagnosis of Mycobacterium bovis is important in the control of transmission to the human population and also for treatment since M. bovis is naturally resistant to pyrazinamide. Eleven clinical isolates from the Fernando Fonseca Hospital with Spoligotypes indicative of M. bovis, through the absence of spacers 39-43 but that also counted with the absence of spacer 38, were analyzed. For the identification of these strains, the phenotypic analysis of pyrazinamide resistance and study of the polymorphisms of the pncA and gyrB genes were carried out. The study of the pncA polymorphism revealed that the strains analyzed did not contain the M. bovis specific mutation. In relation the gyrB polymorphisms, using the GenoTypeO MTBC kit, the strains were identified as belonging to the group M. tuberculosis, M. africanum subtipo II e M. canetti. The present investigation enabled us to define new genotypes on which future bacteriological studies should be based. Amongst these the study of the pncA polymorphism was considered important due to the immediate practical implications for the clinician. Evaluating transmission and defining groups of risk is an objective for which support from the veterinary services is considered relevant. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-01-01T00:00:00Z 2005-01-01T00:00:00Z 2012-08-10T14:50:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.10/651 |
url |
http://hdl.handle.net/10400.10/651 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Rev Port Pneumol. 2005 Nov-Dec;11(6):533-56 0873-2159 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade Portuguesa de Pneumologia |
publisher.none.fl_str_mv |
Sociedade Portuguesa de Pneumologia |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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