Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells
Main Author: | |
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Publication Date: | 2012 |
Other Authors: | , , , |
Format: | Article |
Language: | por |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/10316/18513 https://doi.org/10.1111/j.1749-6632.2002.tb04190.x |
Summary: | Cell death and reactive oxygen species production have been suggested to be involved in neurodegeneration induced by the drugs of abuse. In this study we analyze the toxicity of the following drugs of abuse: heroin, morphine, d-amphetamine, and cocaine in undifferentiated PC12 cells, used as dopaminergic neuronal models. Our data show that opioid drugs (heroin and morphine) are more toxic than stimulant drugs (d-amphetamine and cocaine). Toxic effects induced by heroin are associated with a decrease in intracellular dopamine, an increase in DOPAC levels, and the formation of ROS, whereas toxic effects induced by amphetamine are associated with a decrease in intracellular dopamine and in ATP/ADP levels. In contrast with cocaine, both amphetamine and heroin induced features of apoptosis. The data suggest that the death of cultured PC12 cells induced by the drugs of abuse is correlated with a decrease in intracellular dopamine levels, which can be associated with an increased dopamine turnover and oxidative cell injury. |
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Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 CellsCell death and reactive oxygen species production have been suggested to be involved in neurodegeneration induced by the drugs of abuse. In this study we analyze the toxicity of the following drugs of abuse: heroin, morphine, d-amphetamine, and cocaine in undifferentiated PC12 cells, used as dopaminergic neuronal models. Our data show that opioid drugs (heroin and morphine) are more toxic than stimulant drugs (d-amphetamine and cocaine). Toxic effects induced by heroin are associated with a decrease in intracellular dopamine, an increase in DOPAC levels, and the formation of ROS, whereas toxic effects induced by amphetamine are associated with a decrease in intracellular dopamine and in ATP/ADP levels. In contrast with cocaine, both amphetamine and heroin induced features of apoptosis. The data suggest that the death of cultured PC12 cells induced by the drugs of abuse is correlated with a decrease in intracellular dopamine levels, which can be associated with an increased dopamine turnover and oxidative cell injury.2012-03-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/18513http://hdl.handle.net/10316/18513https://doi.org/10.1111/j.1749-6632.2002.tb04190.xporhttp://onlinelibrary.wiley.com/doi/10.1111/j.1749-6632.2002.tb04190.x/abstractOliveira, M. TeresaRego, A. CristinaMorgadinho, M. TeresaMacedo, Tice R.Oliveira, Catarina R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-09-30T09:22:27Zoai:estudogeral.uc.pt:10316/18513Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:53:34.101802Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells |
title |
Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells |
spellingShingle |
Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells Oliveira, M. Teresa |
title_short |
Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells |
title_full |
Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells |
title_fullStr |
Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells |
title_full_unstemmed |
Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells |
title_sort |
Toxic Effects of Opioid and Stimulant Drugs on Undifferentiated PC12 Cells |
author |
Oliveira, M. Teresa |
author_facet |
Oliveira, M. Teresa Rego, A. Cristina Morgadinho, M. Teresa Macedo, Tice R. Oliveira, Catarina R. |
author_role |
author |
author2 |
Rego, A. Cristina Morgadinho, M. Teresa Macedo, Tice R. Oliveira, Catarina R. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Oliveira, M. Teresa Rego, A. Cristina Morgadinho, M. Teresa Macedo, Tice R. Oliveira, Catarina R. |
description |
Cell death and reactive oxygen species production have been suggested to be involved in neurodegeneration induced by the drugs of abuse. In this study we analyze the toxicity of the following drugs of abuse: heroin, morphine, d-amphetamine, and cocaine in undifferentiated PC12 cells, used as dopaminergic neuronal models. Our data show that opioid drugs (heroin and morphine) are more toxic than stimulant drugs (d-amphetamine and cocaine). Toxic effects induced by heroin are associated with a decrease in intracellular dopamine, an increase in DOPAC levels, and the formation of ROS, whereas toxic effects induced by amphetamine are associated with a decrease in intracellular dopamine and in ATP/ADP levels. In contrast with cocaine, both amphetamine and heroin induced features of apoptosis. The data suggest that the death of cultured PC12 cells induced by the drugs of abuse is correlated with a decrease in intracellular dopamine levels, which can be associated with an increased dopamine turnover and oxidative cell injury. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-03-08 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/18513 http://hdl.handle.net/10316/18513 https://doi.org/10.1111/j.1749-6632.2002.tb04190.x |
url |
http://hdl.handle.net/10316/18513 https://doi.org/10.1111/j.1749-6632.2002.tb04190.x |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
http://onlinelibrary.wiley.com/doi/10.1111/j.1749-6632.2002.tb04190.x/abstract |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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