Detection and quantification of fluconazole within Candida glabrata biofilms
Main Author: | |
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Publication Date: | 2015 |
Other Authors: | , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/35207 |
Summary: | Candida infections are often associated with biofilms and consequent high resistance to most common drugs (e.g. azoles). These resistance mechanisms are not only associated with the biofilm yeast physiology, but also with the presence of a diffusional barrier imposed by the biofilm matrix; however, the real biochemical role of the biofilm components remains very unclear. So, in order to further clarify this issue, we intend to determine, for the first time, fluconazole in biofilms within both supernatants and matrices. Candida biofilms were formed in the presence of fluconazole, and it was recovered from both supernatant and matrix cell-free fractions. Then, high-pressure liquid chromatography was used to identify and quantify the amount of drug that was present in the two fractions. Moreover, this study also showed that the presence of fluconazole in both fractions indicated that the drug administrated did not completely reach the cells, so this phenomena can easily be associated with lower biofilm susceptibility, since the drug administered did not completely reach the cells. |
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Detection and quantification of fluconazole within Candida glabrata biofilmsCandidaBiofilm matrixAntifungalMethodResistanceScience & TechnologyCandida infections are often associated with biofilms and consequent high resistance to most common drugs (e.g. azoles). These resistance mechanisms are not only associated with the biofilm yeast physiology, but also with the presence of a diffusional barrier imposed by the biofilm matrix; however, the real biochemical role of the biofilm components remains very unclear. So, in order to further clarify this issue, we intend to determine, for the first time, fluconazole in biofilms within both supernatants and matrices. Candida biofilms were formed in the presence of fluconazole, and it was recovered from both supernatant and matrix cell-free fractions. Then, high-pressure liquid chromatography was used to identify and quantify the amount of drug that was present in the two fractions. Moreover, this study also showed that the presence of fluconazole in both fractions indicated that the drug administrated did not completely reach the cells, so this phenomena can easily be associated with lower biofilm susceptibility, since the drug administered did not completely reach the cells.This work was supported by the Programa Operacional, Fatores de competitividade-COMPETE and by national funds through FCT-Fundacao para a Ciencia e a Tecnologia on the scope of the projects FCT PTDC/SAU-MIC/119069/2010, RECI/EBB-EBI/0179/2012, PEst-OE/EQB/LA0023/2013 and Celia F. Rodrigue's SFRH/BD/93078/2013 PhD grant. The authors thank the Project "BioHealth-Biotechnology and Bioengineering approaches to improve health quality,'' Ref. NORTE-07-0124-FEDER-000027, co-funded by the Programa Operacional Regional do Norte (ON. 2-O Novo Norte), QREN, FEDER. We also would like to acknowledge Pfizer (R), S.A., for the kindly donation of fluconazole.KluwerUniversidade do MinhoRodrigues, Célia F.Silva, Sónia CarinaAzeredo, JoanaHenriques, Mariana2015-01-232015-01-23T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/35207engRodrigues, Célia F.; Silva, Sónia Carina; Azeredo, Joana; Henriques, Mariana, Detection and quantification of fluconazole within Candida glabrata biofilms. Mycopathologia, 179(5-6), 391-395, 20151573-08320301-486X10.1007/s11046-015-9862-925614085info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:32Zoai:repositorium.sdum.uminho.pt:1822/35207Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:26.954695Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Detection and quantification of fluconazole within Candida glabrata biofilms |
title |
Detection and quantification of fluconazole within Candida glabrata biofilms |
spellingShingle |
Detection and quantification of fluconazole within Candida glabrata biofilms Rodrigues, Célia F. Candida Biofilm matrix Antifungal Method Resistance Science & Technology |
title_short |
Detection and quantification of fluconazole within Candida glabrata biofilms |
title_full |
Detection and quantification of fluconazole within Candida glabrata biofilms |
title_fullStr |
Detection and quantification of fluconazole within Candida glabrata biofilms |
title_full_unstemmed |
Detection and quantification of fluconazole within Candida glabrata biofilms |
title_sort |
Detection and quantification of fluconazole within Candida glabrata biofilms |
author |
Rodrigues, Célia F. |
author_facet |
Rodrigues, Célia F. Silva, Sónia Carina Azeredo, Joana Henriques, Mariana |
author_role |
author |
author2 |
Silva, Sónia Carina Azeredo, Joana Henriques, Mariana |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Rodrigues, Célia F. Silva, Sónia Carina Azeredo, Joana Henriques, Mariana |
dc.subject.por.fl_str_mv |
Candida Biofilm matrix Antifungal Method Resistance Science & Technology |
topic |
Candida Biofilm matrix Antifungal Method Resistance Science & Technology |
description |
Candida infections are often associated with biofilms and consequent high resistance to most common drugs (e.g. azoles). These resistance mechanisms are not only associated with the biofilm yeast physiology, but also with the presence of a diffusional barrier imposed by the biofilm matrix; however, the real biochemical role of the biofilm components remains very unclear. So, in order to further clarify this issue, we intend to determine, for the first time, fluconazole in biofilms within both supernatants and matrices. Candida biofilms were formed in the presence of fluconazole, and it was recovered from both supernatant and matrix cell-free fractions. Then, high-pressure liquid chromatography was used to identify and quantify the amount of drug that was present in the two fractions. Moreover, this study also showed that the presence of fluconazole in both fractions indicated that the drug administrated did not completely reach the cells, so this phenomena can easily be associated with lower biofilm susceptibility, since the drug administered did not completely reach the cells. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-01-23 2015-01-23T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/35207 |
url |
http://hdl.handle.net/1822/35207 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Rodrigues, Célia F.; Silva, Sónia Carina; Azeredo, Joana; Henriques, Mariana, Detection and quantification of fluconazole within Candida glabrata biofilms. Mycopathologia, 179(5-6), 391-395, 2015 1573-0832 0301-486X 10.1007/s11046-015-9862-9 25614085 |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Kluwer |
publisher.none.fl_str_mv |
Kluwer |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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