Flow cytometry for age assessment of a yeast population and its application in beer fermentations
Autor(a) principal: | |
---|---|
Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/10194 |
Resumo: | An expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed a good correlation and allowed routine cell age estimation by flow cytometry. The developed method was applied for yeast age control in traditional batch and continuous beer fermentations. At the pitching rates used in industrial beer fermentations, our results support former findings by locating a gradient of increasing yeast age from the top to the bottom zone of the fermenter cone. The results also indicate that in continuous beer fermentation, the increasing bud scar fluorescence of immobilized cells could help to schedule the replacement of aged biomass, prior to loss of viability or deterioration of process performance and product quality. |
id |
RCAP_cb84a578d2f049b26da81c7dc0cb8c31 |
---|---|
oai_identifier_str |
oai:repositorium.sdum.uminho.pt:1822/10194 |
network_acronym_str |
RCAP |
network_name_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository_id_str |
7160 |
spelling |
Flow cytometry for age assessment of a yeast population and its application in beer fermentationsAgingBeerBud scarFlow cytometryStainingYeastScience & TechnologyAn expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed a good correlation and allowed routine cell age estimation by flow cytometry. The developed method was applied for yeast age control in traditional batch and continuous beer fermentations. At the pitching rates used in industrial beer fermentations, our results support former findings by locating a gradient of increasing yeast age from the top to the bottom zone of the fermenter cone. The results also indicate that in continuous beer fermentation, the increasing bud scar fluorescence of immobilized cells could help to schedule the replacement of aged biomass, prior to loss of viability or deterioration of process performance and product quality.Grant Agency of the Czech Republic (GAČR 104/06/1418) and MŠMT (MSM 6046137305, Czech Republic).Institute of Brewing & DistillingUniversidade do MinhoKuřec, MichalBaszczyňski, MartinLehnert, RadekMota, AndréTeixeira, J. A.Brányik, Tomáš20092009-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/10194eng"Journal of the Institute of Brewing". ISSN 0046-9750. 115:3 (2009) 253-258.0046-975010.1002/j.2050-0416.2009.tb00377.xinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:57Zoai:repositorium.sdum.uminho.pt:1822/10194Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:50:59.058776Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
title |
Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
spellingShingle |
Flow cytometry for age assessment of a yeast population and its application in beer fermentations Kuřec, Michal Aging Beer Bud scar Flow cytometry Staining Yeast Science & Technology |
title_short |
Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
title_full |
Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
title_fullStr |
Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
title_full_unstemmed |
Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
title_sort |
Flow cytometry for age assessment of a yeast population and its application in beer fermentations |
author |
Kuřec, Michal |
author_facet |
Kuřec, Michal Baszczyňski, Martin Lehnert, Radek Mota, André Teixeira, J. A. Brányik, Tomáš |
author_role |
author |
author2 |
Baszczyňski, Martin Lehnert, Radek Mota, André Teixeira, J. A. Brányik, Tomáš |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Kuřec, Michal Baszczyňski, Martin Lehnert, Radek Mota, André Teixeira, J. A. Brányik, Tomáš |
dc.subject.por.fl_str_mv |
Aging Beer Bud scar Flow cytometry Staining Yeast Science & Technology |
topic |
Aging Beer Bud scar Flow cytometry Staining Yeast Science & Technology |
description |
An expeditious method of yeast age estimation was developed based on selective bud scar staining (Alexa Fluor 488-labelled wheat-germ agglutinin) and subsequent fluorescence intensity measurement by flow cytometry. The calibration curve resulting from the cytometric determination of average bud scar fluorescence intensities vs. microscopically counted average bud scar numbers of the same cell populations showed a good correlation and allowed routine cell age estimation by flow cytometry. The developed method was applied for yeast age control in traditional batch and continuous beer fermentations. At the pitching rates used in industrial beer fermentations, our results support former findings by locating a gradient of increasing yeast age from the top to the bottom zone of the fermenter cone. The results also indicate that in continuous beer fermentation, the increasing bud scar fluorescence of immobilized cells could help to schedule the replacement of aged biomass, prior to loss of viability or deterioration of process performance and product quality. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009 2009-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/10194 |
url |
http://hdl.handle.net/1822/10194 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
"Journal of the Institute of Brewing". ISSN 0046-9750. 115:3 (2009) 253-258. 0046-9750 10.1002/j.2050-0416.2009.tb00377.x |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Institute of Brewing & Distilling |
publisher.none.fl_str_mv |
Institute of Brewing & Distilling |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
repository.mail.fl_str_mv |
|
_version_ |
1799133096250966016 |