Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/25720 |
Resumo: | Timely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response to conformational transitions of proteins, are of great interest in cancer research and treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-dimensional (3D) matrix. The proposed SF hydrogels presented a viscoelastic nature of injectable hydrogels and spontaneous conformational changes from random coil to β-sheet conformation under physiological conditions. A human neuronal glioblastoma (U251) cell line was used for screening cell encapsulation and in vitro evaluation within the SF hydrogels. The transparent random coil SF hydrogels promoted cell viability and proliferation up to 10 days of culturing, while the crystalline SF hydrogels converted into β-sheet structure induced the formation of TUNEL-positive apoptotic cells. Therefore, this work provides a powerful tool for the investigation of the microenvironment on the programed tumor cells death, by using rapidly responsive SF hydrogels as 3D in vitro tumor models. |
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Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells deathTimely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response to conformational transitions of proteins, are of great interest in cancer research and treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-dimensional (3D) matrix. The proposed SF hydrogels presented a viscoelastic nature of injectable hydrogels and spontaneous conformational changes from random coil to β-sheet conformation under physiological conditions. A human neuronal glioblastoma (U251) cell line was used for screening cell encapsulation and in vitro evaluation within the SF hydrogels. The transparent random coil SF hydrogels promoted cell viability and proliferation up to 10 days of culturing, while the crystalline SF hydrogels converted into β-sheet structure induced the formation of TUNEL-positive apoptotic cells. Therefore, this work provides a powerful tool for the investigation of the microenvironment on the programed tumor cells death, by using rapidly responsive SF hydrogels as 3D in vitro tumor models.Public Library ScienceVeritati - Repositório Institucional da Universidade Católica PortuguesaRibeiro, Viviana P.Silva-Correia, JoanaGonçalves, CristianaPina, SandraRadhouani, HajerMontonen, ToniOliveira, Joaquim M.Oliveira, Ana L.2018-09-20T10:55:16Z20182018-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/25720engRibeiro, V.P., Silva-Correia, J., Gonçalves, C., Pina, S., Radhouani, H., Montonen, T., … Oliveira, J.M. (2018). Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death. PLoS ONE 13(4), e01944411932-620310.1371/journal.pone.019444129617395WOS:000429203800031info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-12T17:31:07ZPortal AgregadorONG |
dc.title.none.fl_str_mv |
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death |
title |
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death |
spellingShingle |
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death Ribeiro, Viviana P. |
title_short |
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death |
title_full |
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death |
title_fullStr |
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death |
title_full_unstemmed |
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death |
title_sort |
Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death |
author |
Ribeiro, Viviana P. |
author_facet |
Ribeiro, Viviana P. Silva-Correia, Joana Gonçalves, Cristiana Pina, Sandra Radhouani, Hajer Montonen, Toni Oliveira, Joaquim M. Oliveira, Ana L. |
author_role |
author |
author2 |
Silva-Correia, Joana Gonçalves, Cristiana Pina, Sandra Radhouani, Hajer Montonen, Toni Oliveira, Joaquim M. Oliveira, Ana L. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Ribeiro, Viviana P. Silva-Correia, Joana Gonçalves, Cristiana Pina, Sandra Radhouani, Hajer Montonen, Toni Oliveira, Joaquim M. Oliveira, Ana L. |
description |
Timely and spatially-regulated injectable hydrogels, able to suppress growing tumors in response to conformational transitions of proteins, are of great interest in cancer research and treatment. Herein, we report rapidly responsive silk fibroin (SF) hydrogels formed by a horseradish peroxidase (HRP) crosslinking reaction at physiological conditions, and demonstrate their use as an artificial biomimetic three-dimensional (3D) matrix. The proposed SF hydrogels presented a viscoelastic nature of injectable hydrogels and spontaneous conformational changes from random coil to β-sheet conformation under physiological conditions. A human neuronal glioblastoma (U251) cell line was used for screening cell encapsulation and in vitro evaluation within the SF hydrogels. The transparent random coil SF hydrogels promoted cell viability and proliferation up to 10 days of culturing, while the crystalline SF hydrogels converted into β-sheet structure induced the formation of TUNEL-positive apoptotic cells. Therefore, this work provides a powerful tool for the investigation of the microenvironment on the programed tumor cells death, by using rapidly responsive SF hydrogels as 3D in vitro tumor models. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-09-20T10:55:16Z 2018 2018-01-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/25720 |
url |
http://hdl.handle.net/10400.14/25720 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ribeiro, V.P., Silva-Correia, J., Gonçalves, C., Pina, S., Radhouani, H., Montonen, T., … Oliveira, J.M. (2018). Rapidly responsive silk fibroin hydrogels as an artificial matrix for the programmed tumor cells death. PLoS ONE 13(4), e0194441 1932-6203 10.1371/journal.pone.0194441 29617395 WOS:000429203800031 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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repository.mail.fl_str_mv |
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1777303174945701888 |