Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behavior
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/23789 |
Resumo: | Objective. In the treatment of periodontal defects, composite membranes might be applied to protect the injured area and simultaneously stimulate tissue regeneration. This work describes the development and characterization of poly(d,l-lactic acid)/Bioglass® (PDLLA/BG) composite membranes with asymmetric bioactivity. We hypothesized that the presence of BG microparticles could enhance structural and osteoconductivity performance of pure PDLLA membranes. Methods. The membranes were prepared by an adjusted solvent casting method that promoted a non-uniform distribution of the inorganic component along the membrane thickness. In vitro bioactive behavior (precipitation of an apatite layer upon immersion in simulated body fluid, SBF), SEM observation, FTIR, swelling, weight loss and mechanical properties of the developed biomaterials were evaluated. Cell behavior on the membranes was assessed using both human bone marrow stromal cells and human periodontal ligament cells. Results. Just the BG rich face of the composite membranes induced the precipitation of bone-like apatite in SBF, indicating that this biomaterial exhibit asymmetric osteoconductive properties. SEM images, DNA content and metabolic activity quantification revealed an improved cell adhesion and proliferation on the composite membranes. Composite membranes also stimulated cell differentiation, mineralization, and production of extracellular matrix and calcium nodules, suggesting the positive effect of adding the bioactive microparticles in the PDLLA matrix. Significance. The results indicate that the proposed asymmetric PDLLA/BG membranes could have potential to be used in guided tissue regeneration therapies or in orthopedic applications, with improved outcomes. |
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Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behaviorBioglass®MembranePDLLAPeriodontal ligamentTissue engineeringBioglass (R)Science & TechnologyObjective. In the treatment of periodontal defects, composite membranes might be applied to protect the injured area and simultaneously stimulate tissue regeneration. This work describes the development and characterization of poly(d,l-lactic acid)/Bioglass® (PDLLA/BG) composite membranes with asymmetric bioactivity. We hypothesized that the presence of BG microparticles could enhance structural and osteoconductivity performance of pure PDLLA membranes. Methods. The membranes were prepared by an adjusted solvent casting method that promoted a non-uniform distribution of the inorganic component along the membrane thickness. In vitro bioactive behavior (precipitation of an apatite layer upon immersion in simulated body fluid, SBF), SEM observation, FTIR, swelling, weight loss and mechanical properties of the developed biomaterials were evaluated. Cell behavior on the membranes was assessed using both human bone marrow stromal cells and human periodontal ligament cells. Results. Just the BG rich face of the composite membranes induced the precipitation of bone-like apatite in SBF, indicating that this biomaterial exhibit asymmetric osteoconductive properties. SEM images, DNA content and metabolic activity quantification revealed an improved cell adhesion and proliferation on the composite membranes. Composite membranes also stimulated cell differentiation, mineralization, and production of extracellular matrix and calcium nodules, suggesting the positive effect of adding the bioactive microparticles in the PDLLA matrix. Significance. The results indicate that the proposed asymmetric PDLLA/BG membranes could have potential to be used in guided tissue regeneration therapies or in orthopedic applications, with improved outcomes.This work was financially supported by Foundation for Science and Technology (FCT) by the project PTDC/CTM-BPC/112774/2009.ElsevierUniversidade do MinhoLeal, Ana IsabelCaridade, S. G.Ma, J.Yu, N.Gomes, Manuela E.Reis, R. L.Jansen, John A.Walboomers, X. F.Mano, J. F.2013-042013-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/23789eng0109-564110.1016/j.dental.2013.01.00923422419http://www.sciencedirect.com/science/article/pii/S0109564113000134info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:14:07Zoai:repositorium.sdum.uminho.pt:1822/23789Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:06:21.913530Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behavior |
title |
Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behavior |
spellingShingle |
Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behavior Leal, Ana Isabel Bioglass® Membrane PDLLA Periodontal ligament Tissue engineering Bioglass (R) Science & Technology |
title_short |
Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behavior |
title_full |
Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behavior |
title_fullStr |
Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behavior |
title_full_unstemmed |
Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behavior |
title_sort |
Asymmetric PDLLA membranes containing Bioglass® for guided tissue regeneration : characterization and in-vitro biological behavior |
author |
Leal, Ana Isabel |
author_facet |
Leal, Ana Isabel Caridade, S. G. Ma, J. Yu, N. Gomes, Manuela E. Reis, R. L. Jansen, John A. Walboomers, X. F. Mano, J. F. |
author_role |
author |
author2 |
Caridade, S. G. Ma, J. Yu, N. Gomes, Manuela E. Reis, R. L. Jansen, John A. Walboomers, X. F. Mano, J. F. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Leal, Ana Isabel Caridade, S. G. Ma, J. Yu, N. Gomes, Manuela E. Reis, R. L. Jansen, John A. Walboomers, X. F. Mano, J. F. |
dc.subject.por.fl_str_mv |
Bioglass® Membrane PDLLA Periodontal ligament Tissue engineering Bioglass (R) Science & Technology |
topic |
Bioglass® Membrane PDLLA Periodontal ligament Tissue engineering Bioglass (R) Science & Technology |
description |
Objective. In the treatment of periodontal defects, composite membranes might be applied to protect the injured area and simultaneously stimulate tissue regeneration. This work describes the development and characterization of poly(d,l-lactic acid)/Bioglass® (PDLLA/BG) composite membranes with asymmetric bioactivity. We hypothesized that the presence of BG microparticles could enhance structural and osteoconductivity performance of pure PDLLA membranes. Methods. The membranes were prepared by an adjusted solvent casting method that promoted a non-uniform distribution of the inorganic component along the membrane thickness. In vitro bioactive behavior (precipitation of an apatite layer upon immersion in simulated body fluid, SBF), SEM observation, FTIR, swelling, weight loss and mechanical properties of the developed biomaterials were evaluated. Cell behavior on the membranes was assessed using both human bone marrow stromal cells and human periodontal ligament cells. Results. Just the BG rich face of the composite membranes induced the precipitation of bone-like apatite in SBF, indicating that this biomaterial exhibit asymmetric osteoconductive properties. SEM images, DNA content and metabolic activity quantification revealed an improved cell adhesion and proliferation on the composite membranes. Composite membranes also stimulated cell differentiation, mineralization, and production of extracellular matrix and calcium nodules, suggesting the positive effect of adding the bioactive microparticles in the PDLLA matrix. Significance. The results indicate that the proposed asymmetric PDLLA/BG membranes could have potential to be used in guided tissue regeneration therapies or in orthopedic applications, with improved outcomes. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-04 2013-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/23789 |
url |
http://hdl.handle.net/1822/23789 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
0109-5641 10.1016/j.dental.2013.01.009 23422419 http://www.sciencedirect.com/science/article/pii/S0109564113000134 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
instname_str |
Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
instacron_str |
RCAAP |
institution |
RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
repository.name.fl_str_mv |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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1799132479005655040 |