New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358
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Publication Date: | 2017 |
Other Authors: | , , , , , , |
Format: | Article |
Language: | eng |
Source: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Download full: | http://hdl.handle.net/1822/46562 |
Summary: | In this work, biosurfactant production by several yeast strains was evaluated using different culture media. The best results were obtained with the strain Wickerhamomyces anomalus CCMA 0358 growing in a culture medium containing glucose (1 g/L) and olive oil (20 g/L) as carbon sources. This strain produced 2.6 g of biosurfactant per liter after 24 hours of growth. The crude biosurfactant reduced the surface tension of water to values around 31 mN/m, and its critical micelle concentration was 0.9 mg/mL. This biosurfactant was characterized through mass spectrometry (MS), and nuclear magnetic resonance (NMR) as a mixture of two different glycolipids, comprising a sugar moiety linked to one or three molecules of oleic acid. To the best of our knowledge, these biosurfactants are structurally different from those previously reported. Furthermore, the crude biosurfactant exhibited antimicrobial activity against several microorganisms, including the pathogens Candida albicans, Escherichia coli, Staphylococcus epidermidis and Streptococcus agalactiae, which opens the possibility for its use in several biomedical applications. |
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New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358BiosurfactantGlycolipidAntimicrobial activityCritical micelle concentrationWickerhamomyces anomalusScience & TechnologyIn this work, biosurfactant production by several yeast strains was evaluated using different culture media. The best results were obtained with the strain Wickerhamomyces anomalus CCMA 0358 growing in a culture medium containing glucose (1 g/L) and olive oil (20 g/L) as carbon sources. This strain produced 2.6 g of biosurfactant per liter after 24 hours of growth. The crude biosurfactant reduced the surface tension of water to values around 31 mN/m, and its critical micelle concentration was 0.9 mg/mL. This biosurfactant was characterized through mass spectrometry (MS), and nuclear magnetic resonance (NMR) as a mixture of two different glycolipids, comprising a sugar moiety linked to one or three molecules of oleic acid. To the best of our knowledge, these biosurfactants are structurally different from those previously reported. Furthermore, the crude biosurfactant exhibited antimicrobial activity against several microorganisms, including the pathogens Candida albicans, Escherichia coli, Staphylococcus epidermidis and Streptococcus agalactiae, which opens the possibility for its use in several biomedical applications.This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684); and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 − Programa Operacional Regional do Norte. The authors also thank the FCT for the financial support under the scope of the Project RECI/BBB-EBI/0179/2012 (FCOMP-01-0124-FEDER-027462).info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoSouza, Karla S. TeixeiraGudiña, Eduardo J.Azevedo, ZéliaFreitas, Victor deSchwan, Rosane F.Rodrigues, L. R.Dias, Disney R.Teixeira, J. A.2017-062017-06-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/46562engSouza, Karla S. Teixeira; Gudiña, Eduardo J.; Azevedo, Zélia; Freitas, Victor de; Schwan, Rosane F.; Rodrigues, Lígia R.; Dias, Disney R.; Teixeira, José A., New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358. Colloids and Surfaces B: Biointerfaces, 154, 373-382, 20170927-77650927-776510.1016/j.colsurfb.2017.03.04128376391http://www.journals.elsevier.com/colloids-and-surfaces-b-biointerfaces/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:51:58Zoai:repositorium.sdum.uminho.pt:1822/46562Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:51:00.379535Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358 |
title |
New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358 |
spellingShingle |
New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358 Souza, Karla S. Teixeira Biosurfactant Glycolipid Antimicrobial activity Critical micelle concentration Wickerhamomyces anomalus Science & Technology |
title_short |
New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358 |
title_full |
New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358 |
title_fullStr |
New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358 |
title_full_unstemmed |
New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358 |
title_sort |
New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358 |
author |
Souza, Karla S. Teixeira |
author_facet |
Souza, Karla S. Teixeira Gudiña, Eduardo J. Azevedo, Zélia Freitas, Victor de Schwan, Rosane F. Rodrigues, L. R. Dias, Disney R. Teixeira, J. A. |
author_role |
author |
author2 |
Gudiña, Eduardo J. Azevedo, Zélia Freitas, Victor de Schwan, Rosane F. Rodrigues, L. R. Dias, Disney R. Teixeira, J. A. |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Souza, Karla S. Teixeira Gudiña, Eduardo J. Azevedo, Zélia Freitas, Victor de Schwan, Rosane F. Rodrigues, L. R. Dias, Disney R. Teixeira, J. A. |
dc.subject.por.fl_str_mv |
Biosurfactant Glycolipid Antimicrobial activity Critical micelle concentration Wickerhamomyces anomalus Science & Technology |
topic |
Biosurfactant Glycolipid Antimicrobial activity Critical micelle concentration Wickerhamomyces anomalus Science & Technology |
description |
In this work, biosurfactant production by several yeast strains was evaluated using different culture media. The best results were obtained with the strain Wickerhamomyces anomalus CCMA 0358 growing in a culture medium containing glucose (1 g/L) and olive oil (20 g/L) as carbon sources. This strain produced 2.6 g of biosurfactant per liter after 24 hours of growth. The crude biosurfactant reduced the surface tension of water to values around 31 mN/m, and its critical micelle concentration was 0.9 mg/mL. This biosurfactant was characterized through mass spectrometry (MS), and nuclear magnetic resonance (NMR) as a mixture of two different glycolipids, comprising a sugar moiety linked to one or three molecules of oleic acid. To the best of our knowledge, these biosurfactants are structurally different from those previously reported. Furthermore, the crude biosurfactant exhibited antimicrobial activity against several microorganisms, including the pathogens Candida albicans, Escherichia coli, Staphylococcus epidermidis and Streptococcus agalactiae, which opens the possibility for its use in several biomedical applications. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-06 2017-06-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/46562 |
url |
http://hdl.handle.net/1822/46562 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Souza, Karla S. Teixeira; Gudiña, Eduardo J.; Azevedo, Zélia; Freitas, Victor de; Schwan, Rosane F.; Rodrigues, Lígia R.; Dias, Disney R.; Teixeira, José A., New glycolipid biosurfactants produced by the yeast strain Wickerhamomyces anomalus CCMA 0358. Colloids and Surfaces B: Biointerfaces, 154, 373-382, 2017 0927-7765 0927-7765 10.1016/j.colsurfb.2017.03.041 28376391 http://www.journals.elsevier.com/colloids-and-surfaces-b-biointerfaces/ |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier |
publisher.none.fl_str_mv |
Elsevier |
dc.source.none.fl_str_mv |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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