pH-induced modulation of Vibrio fischeri population life cycle
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/1822/74297 |
Resumo: | Commonly used as biological chemosensors in toxicity assays, Vibrio fischeri bacteria were systematically characterized using complementary physicochemical and biological techniques to elucidate the evolution of their properties under varying environmental conditions. Changing the pH above or below the optimal pH 7 was used to model the long-term stress that would be experienced by V. fischeri in environmental toxicology assays. The spectral shape of bioluminescence and cell-surface charge during the exponential growth phase were largely unaffected by pH changes. The pH-induced modulation of V. fischeri growth, monitored via the optical density (OD), was moderate. In contrast, the concomitant changes in the time-profiles of their bioluminescence, which is used as the readout in assays, were more significant. Imaging at discrete timepoints by scanning electron microscopy (SEM) and helium-ion microscopy (HIM) revealed that mature V. fischeri cells maintained a rod-shaped morphology with the average length of 2.2 ± 1 µm and diameter of 0.6 ± 0.1 µm. Detailed morphological analysis revealed subpopulations of rods having aspect ratios significantly larger than those of average individuals, suggesting the use of such elongated rods as an indicator of the multigenerational environmental stress. The observed modulation of bioluminescence and morphology supports the suitability of V. fischeri as biological chemosensors for both rapid and long-term assays, including under environmental conditions that can modify the physicochemical properties of novel anthropogenic pollutants, such as nanomaterials and especially stimulus-responsive nanomaterials. |
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pH-induced modulation of Vibrio fischeri population life cycleVibrio fischeriBioluminescenceCell-surface chargeScanning electron microscopyHelium-ion microscopyZeta potentialCell morphologyCell-size distributionEnvironmental stressCiências Agrárias::Biotecnologia Agrária e AlimentarScience & TechnologyCommonly used as biological chemosensors in toxicity assays, Vibrio fischeri bacteria were systematically characterized using complementary physicochemical and biological techniques to elucidate the evolution of their properties under varying environmental conditions. Changing the pH above or below the optimal pH 7 was used to model the long-term stress that would be experienced by V. fischeri in environmental toxicology assays. The spectral shape of bioluminescence and cell-surface charge during the exponential growth phase were largely unaffected by pH changes. The pH-induced modulation of V. fischeri growth, monitored via the optical density (OD), was moderate. In contrast, the concomitant changes in the time-profiles of their bioluminescence, which is used as the readout in assays, were more significant. Imaging at discrete timepoints by scanning electron microscopy (SEM) and helium-ion microscopy (HIM) revealed that mature V. fischeri cells maintained a rod-shaped morphology with the average length of 2.2 ± 1 µm and diameter of 0.6 ± 0.1 µm. Detailed morphological analysis revealed subpopulations of rods having aspect ratios significantly larger than those of average individuals, suggesting the use of such elongated rods as an indicator of the multigenerational environmental stress. The observed modulation of bioluminescence and morphology supports the suitability of V. fischeri as biological chemosensors for both rapid and long-term assays, including under environmental conditions that can modify the physicochemical properties of novel anthropogenic pollutants, such as nanomaterials and especially stimulus-responsive nanomaterials.Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UIDB/04469/2020 unit and COMPETE 2020 (POCI‐ 01‐0145‐FEDER‐006684) and BioTecNorte operation (NORTE‐01‐0145‐FEDER‐000004) funded by European Regional Development Fund under the scope of Norte2020 Programa Operacional Re‐ gional do Norte. A.R.S. holds an FCT fellowship SFRH/BD/131905/2017. Measurements using the helium‐ion microscope were supported by the Karlsruhe Nano Micro Facility (KNMF) of the Karls‐ ruhe Institute of Technology (KIT) under projects 2016‐015‐010689 and 2018‐019‐021475info:eu-repo/semantics/publishedVersionMDPIUniversidade do MinhoSilva, Ana Rita PereiraSousa, Cláudia Sofia CunhaExner, DanielaRuth, SchwaigerAlves, M. M.Petrovykh, Dmitri Y.Pereira, Luciana2021-10-052021-10-05T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/74297engSilva, A. R.; Sousa, Cláudia; Daniela Exner; Ruth Schwaiger; Alves, M. Madalena; Petrovykh, Dmitri Y.; Pereira, Luciana, pH-induced modulation of Vibrio fischeri population life cycle. Chemosensors, 9(10), 283, 20212227-904010.3390/chemosensors9100283https://www.mdpi.com/2227-9040/9/10/283info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:18:44Zoai:repositorium.sdum.uminho.pt:1822/74297Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T19:11:34.842963Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
pH-induced modulation of Vibrio fischeri population life cycle |
title |
pH-induced modulation of Vibrio fischeri population life cycle |
spellingShingle |
pH-induced modulation of Vibrio fischeri population life cycle Silva, Ana Rita Pereira Vibrio fischeri Bioluminescence Cell-surface charge Scanning electron microscopy Helium-ion microscopy Zeta potential Cell morphology Cell-size distribution Environmental stress Ciências Agrárias::Biotecnologia Agrária e Alimentar Science & Technology |
title_short |
pH-induced modulation of Vibrio fischeri population life cycle |
title_full |
pH-induced modulation of Vibrio fischeri population life cycle |
title_fullStr |
pH-induced modulation of Vibrio fischeri population life cycle |
title_full_unstemmed |
pH-induced modulation of Vibrio fischeri population life cycle |
title_sort |
pH-induced modulation of Vibrio fischeri population life cycle |
author |
Silva, Ana Rita Pereira |
author_facet |
Silva, Ana Rita Pereira Sousa, Cláudia Sofia Cunha Exner, Daniela Ruth, Schwaiger Alves, M. M. Petrovykh, Dmitri Y. Pereira, Luciana |
author_role |
author |
author2 |
Sousa, Cláudia Sofia Cunha Exner, Daniela Ruth, Schwaiger Alves, M. M. Petrovykh, Dmitri Y. Pereira, Luciana |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade do Minho |
dc.contributor.author.fl_str_mv |
Silva, Ana Rita Pereira Sousa, Cláudia Sofia Cunha Exner, Daniela Ruth, Schwaiger Alves, M. M. Petrovykh, Dmitri Y. Pereira, Luciana |
dc.subject.por.fl_str_mv |
Vibrio fischeri Bioluminescence Cell-surface charge Scanning electron microscopy Helium-ion microscopy Zeta potential Cell morphology Cell-size distribution Environmental stress Ciências Agrárias::Biotecnologia Agrária e Alimentar Science & Technology |
topic |
Vibrio fischeri Bioluminescence Cell-surface charge Scanning electron microscopy Helium-ion microscopy Zeta potential Cell morphology Cell-size distribution Environmental stress Ciências Agrárias::Biotecnologia Agrária e Alimentar Science & Technology |
description |
Commonly used as biological chemosensors in toxicity assays, Vibrio fischeri bacteria were systematically characterized using complementary physicochemical and biological techniques to elucidate the evolution of their properties under varying environmental conditions. Changing the pH above or below the optimal pH 7 was used to model the long-term stress that would be experienced by V. fischeri in environmental toxicology assays. The spectral shape of bioluminescence and cell-surface charge during the exponential growth phase were largely unaffected by pH changes. The pH-induced modulation of V. fischeri growth, monitored via the optical density (OD), was moderate. In contrast, the concomitant changes in the time-profiles of their bioluminescence, which is used as the readout in assays, were more significant. Imaging at discrete timepoints by scanning electron microscopy (SEM) and helium-ion microscopy (HIM) revealed that mature V. fischeri cells maintained a rod-shaped morphology with the average length of 2.2 ± 1 µm and diameter of 0.6 ± 0.1 µm. Detailed morphological analysis revealed subpopulations of rods having aspect ratios significantly larger than those of average individuals, suggesting the use of such elongated rods as an indicator of the multigenerational environmental stress. The observed modulation of bioluminescence and morphology supports the suitability of V. fischeri as biological chemosensors for both rapid and long-term assays, including under environmental conditions that can modify the physicochemical properties of novel anthropogenic pollutants, such as nanomaterials and especially stimulus-responsive nanomaterials. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-10-05 2021-10-05T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/1822/74297 |
url |
http://hdl.handle.net/1822/74297 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Silva, A. R.; Sousa, Cláudia; Daniela Exner; Ruth Schwaiger; Alves, M. Madalena; Petrovykh, Dmitri Y.; Pereira, Luciana, pH-induced modulation of Vibrio fischeri population life cycle. Chemosensors, 9(10), 283, 2021 2227-9040 10.3390/chemosensors9100283 https://www.mdpi.com/2227-9040/9/10/283 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
MDPI |
publisher.none.fl_str_mv |
MDPI |
dc.source.none.fl_str_mv |
reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
reponame_str |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
collection |
Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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