Overview on protein extraction and purification using ionic-liquid-based processes

• Autor(a) principal: Nunes, João C. F.
• Data de Publicação: 2021
• Outros Autores: Almeida, Mafalda R., Faria, Joaquim L., Silva, Cláudia G., Neves, Márcia C., Freire, Mara G., Tavares, Ana P. M.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
• Texto Completo: http://hdl.handle.net/10773/32798
• topic: Ionic liquids; Aqueous biphasic systems; Solid-phase extractions; Poly(ionic liquid)s; Supported ionic liquid phases; Crystallization; Extraction; Purification; Proteins

Proteins are one the most widely studied biomolecules with diverse functions and applications. Aiming at overcoming the current drawbacks of purification processes of proteins, the introduction of ionic liquids (ILs) has been a hot topic of research. ILs have been applied in the creation of aqueous biphasic systems (IL-based ABS), solid-phase extractions through poly(ionic liquid)s (PILs) and supported ionic-liquid phases (SILPs), and in the crystallization of proteins. In this sense, ILs have emerged as solvents, electrolytes or adjuvants, or as supported materials to tune the adsorption/affinity capacity aiming at developing an efficient, cost-effective, sustainable and green IL-based process for protein extraction. This review discusses different IL-based processes in the extraction and purification of proteins in the past years, namely IL-based aqueous biphasic systems (IL-based ABS), solid-phase extractions through PILs and SILPs, and protein crystallization. The type and structure of ILs applied and their influence in the different processes performance are also discussed.