Overview on protein extraction and purification using ionic-liquid-based processes

Detalhes bibliográficos
Autor(a) principal: Nunes, João C. F.
Data de Publicação: 2021
Outros Autores: Almeida, Mafalda R., Faria, Joaquim L., Silva, Cláudia G., Neves, Márcia C., Freire, Mara G., Tavares, Ana P. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/32798
Resumo: Proteins are one the most widely studied biomolecules with diverse functions and applications. Aiming at overcoming the current drawbacks of purification processes of proteins, the introduction of ionic liquids (ILs) has been a hot topic of research. ILs have been applied in the creation of aqueous biphasic systems (IL-based ABS), solid-phase extractions through poly(ionic liquid)s (PILs) and supported ionic-liquid phases (SILPs), and in the crystallization of proteins. In this sense, ILs have emerged as solvents, electrolytes or adjuvants, or as supported materials to tune the adsorption/affinity capacity aiming at developing an efficient, cost-effective, sustainable and green IL-based process for protein extraction. This review discusses different IL-based processes in the extraction and purification of proteins in the past years, namely IL-based aqueous biphasic systems (IL-based ABS), solid-phase extractions through PILs and SILPs, and protein crystallization. The type and structure of ILs applied and their influence in the different processes performance are also discussed.
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spelling Overview on protein extraction and purification using ionic-liquid-based processesIonic liquidsAqueous biphasic systemsSolid-phase extractionsPoly(ionic liquid)sSupported ionic liquid phasesCrystallizationExtractionPurificationProteinsProteins are one the most widely studied biomolecules with diverse functions and applications. Aiming at overcoming the current drawbacks of purification processes of proteins, the introduction of ionic liquids (ILs) has been a hot topic of research. ILs have been applied in the creation of aqueous biphasic systems (IL-based ABS), solid-phase extractions through poly(ionic liquid)s (PILs) and supported ionic-liquid phases (SILPs), and in the crystallization of proteins. In this sense, ILs have emerged as solvents, electrolytes or adjuvants, or as supported materials to tune the adsorption/affinity capacity aiming at developing an efficient, cost-effective, sustainable and green IL-based process for protein extraction. This review discusses different IL-based processes in the extraction and purification of proteins in the past years, namely IL-based aqueous biphasic systems (IL-based ABS), solid-phase extractions through PILs and SILPs, and protein crystallization. The type and structure of ILs applied and their influence in the different processes performance are also discussed.Springer2022-04-08T00:00:00Z2021-04-08T00:00:00Z2021-04-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/32798eng0095-978210.1007/s10953-021-01062-xNunes, João C. F.Almeida, Mafalda R.Faria, Joaquim L.Silva, Cláudia G.Neves, Márcia C.Freire, Mara G.Tavares, Ana P. M.info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:03:09Zoai:ria.ua.pt:10773/32798Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:04:21.635567Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Overview on protein extraction and purification using ionic-liquid-based processes
title Overview on protein extraction and purification using ionic-liquid-based processes
spellingShingle Overview on protein extraction and purification using ionic-liquid-based processes
Nunes, João C. F.
Ionic liquids
Aqueous biphasic systems
Solid-phase extractions
Poly(ionic liquid)s
Supported ionic liquid phases
Crystallization
Extraction
Purification
Proteins
title_short Overview on protein extraction and purification using ionic-liquid-based processes
title_full Overview on protein extraction and purification using ionic-liquid-based processes
title_fullStr Overview on protein extraction and purification using ionic-liquid-based processes
title_full_unstemmed Overview on protein extraction and purification using ionic-liquid-based processes
title_sort Overview on protein extraction and purification using ionic-liquid-based processes
author Nunes, João C. F.
author_facet Nunes, João C. F.
Almeida, Mafalda R.
Faria, Joaquim L.
Silva, Cláudia G.
Neves, Márcia C.
Freire, Mara G.
Tavares, Ana P. M.
author_role author
author2 Almeida, Mafalda R.
Faria, Joaquim L.
Silva, Cláudia G.
Neves, Márcia C.
Freire, Mara G.
Tavares, Ana P. M.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nunes, João C. F.
Almeida, Mafalda R.
Faria, Joaquim L.
Silva, Cláudia G.
Neves, Márcia C.
Freire, Mara G.
Tavares, Ana P. M.
dc.subject.por.fl_str_mv Ionic liquids
Aqueous biphasic systems
Solid-phase extractions
Poly(ionic liquid)s
Supported ionic liquid phases
Crystallization
Extraction
Purification
Proteins
topic Ionic liquids
Aqueous biphasic systems
Solid-phase extractions
Poly(ionic liquid)s
Supported ionic liquid phases
Crystallization
Extraction
Purification
Proteins
description Proteins are one the most widely studied biomolecules with diverse functions and applications. Aiming at overcoming the current drawbacks of purification processes of proteins, the introduction of ionic liquids (ILs) has been a hot topic of research. ILs have been applied in the creation of aqueous biphasic systems (IL-based ABS), solid-phase extractions through poly(ionic liquid)s (PILs) and supported ionic-liquid phases (SILPs), and in the crystallization of proteins. In this sense, ILs have emerged as solvents, electrolytes or adjuvants, or as supported materials to tune the adsorption/affinity capacity aiming at developing an efficient, cost-effective, sustainable and green IL-based process for protein extraction. This review discusses different IL-based processes in the extraction and purification of proteins in the past years, namely IL-based aqueous biphasic systems (IL-based ABS), solid-phase extractions through PILs and SILPs, and protein crystallization. The type and structure of ILs applied and their influence in the different processes performance are also discussed.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-08T00:00:00Z
2021-04-08
2022-04-08T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/32798
url http://hdl.handle.net/10773/32798
dc.language.iso.fl_str_mv eng
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dc.relation.none.fl_str_mv 0095-9782
10.1007/s10953-021-01062-x
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dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
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