Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications

Detalhes bibliográficos
Autor(a) principal: Requicha, J. F.
Data de Publicação: 2016
Outros Autores: Viegas, C. A. A., Hede, Shantesh, Leonor, I. B., Reis, R. L., Gomes, Manuela E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/26834
Resumo: First published: 1 September 2013
id RCAP_dc6bd74dc9f8012fe4e76dae85cb858c
oai_identifier_str oai:repositorium.sdum.uminho.pt:1822/26834
network_acronym_str RCAP
network_name_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository_id_str 7160
spelling Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applicationsBiodegradableGuided tissue regenerationOsteoconductive groupsPeriodontiumScaffoldTissue engineeringScience & TechnologyFirst published: 1 September 2013The inefficacy of the currently used therapies in achieving the regeneration ad integrum of the periodontium stimulates the search for alternative approaches, such as tissue-engineering strategies. Therefore, the core objective of this study was to develop a biodegradable double-layer scaffold for periodontal tissue engineering. The design philosophy was based on a double-layered construct obtained from a blend of starch and poly-ε-caprolactone (30:70 wt%; SPCL). A SPCL fibre mesh functionalized with silanol groups to promote osteogenesis was combined with a SPCL solvent casting membrane aiming at acting as a barrier against the migration of gingival epithelium into the periodontal defect. Each layer of the double-layer scaffolds was characterized in terms of morphology, surface chemical composition, degradation behaviour and mechanical properties. Moreover, the behaviour of seeded/cultured canine adipose-derived stem cells (cASCs) was assessed. In general, the developed double-layered scaffolds demonstrated adequate degradation and mechanical behaviour for the target application. Furthermore, the biological assays revealed that both layers of the scaffold allow adhesion and proliferation of the seeded undifferentiated cASCs, and the incorporation of silanol groups into the fibre-mesh layer enhance the expression of a typical osteogenic marker. This study allowed an innovative construct to be developed, combining a three-dimensional (3D) scaffold with osteoconductive properties and with potential to assist periodontal regeneration, carrying new possible solutions to current clinical needs .The research leading to these results has received funding from the European Union's Seventh Framework Programme (FP7/2007-2013; under Grant Agreement No. REGPOT-CT2012-316331-POLARIS) and from the Portuguese Foundation for Science and Technology (FCT; Grant No, MIT/ECE/0047/2009). Joao Requicha acknowledges the FCT for his PhD scholarship ( Grant No. SFRH/BD/44143/2008).WileyUniversidade do MinhoRequicha, J. F.Viegas, C. A. A.Hede, ShanteshLeonor, I. B.Reis, R. L.Gomes, Manuela E.20162016-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/26834engRequicha, J. F., Viegas, C. A., Hede, S., Leonor, I. B., Reis, R. L., & Gomes, M. E. (2016). Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications. Journal of Tissue Engineering and Regenerative Medicine, 10(5), 392-403. doi: 10.1002/term.18161932-700510.1002/term.181623997028http://onlinelibrary.wiley.com/doi/10.1002/term.1816/abstractinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:54:39Zoai:repositorium.sdum.uminho.pt:1822/26834Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:44:07.972134Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications
title Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications
spellingShingle Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications
Requicha, J. F.
Biodegradable
Guided tissue regeneration
Osteoconductive groups
Periodontium
Scaffold
Tissue engineering
Science & Technology
title_short Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications
title_full Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications
title_fullStr Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications
title_full_unstemmed Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications
title_sort Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications
author Requicha, J. F.
author_facet Requicha, J. F.
Viegas, C. A. A.
Hede, Shantesh
Leonor, I. B.
Reis, R. L.
Gomes, Manuela E.
author_role author
author2 Viegas, C. A. A.
Hede, Shantesh
Leonor, I. B.
Reis, R. L.
Gomes, Manuela E.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Requicha, J. F.
Viegas, C. A. A.
Hede, Shantesh
Leonor, I. B.
Reis, R. L.
Gomes, Manuela E.
dc.subject.por.fl_str_mv Biodegradable
Guided tissue regeneration
Osteoconductive groups
Periodontium
Scaffold
Tissue engineering
Science & Technology
topic Biodegradable
Guided tissue regeneration
Osteoconductive groups
Periodontium
Scaffold
Tissue engineering
Science & Technology
description First published: 1 September 2013
publishDate 2016
dc.date.none.fl_str_mv 2016
2016-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/26834
url http://hdl.handle.net/1822/26834
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Requicha, J. F., Viegas, C. A., Hede, S., Leonor, I. B., Reis, R. L., & Gomes, M. E. (2016). Design and characterization of a biodegradable double-layer scaffold aimed at periodontal tissue-engineering applications. Journal of Tissue Engineering and Regenerative Medicine, 10(5), 392-403. doi: 10.1002/term.1816
1932-7005
10.1002/term.1816
23997028
http://onlinelibrary.wiley.com/doi/10.1002/term.1816/abstract
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
_version_ 1799132189565124608

Registros relacionados