Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons

Detalhes bibliográficos
Autor(a) principal: Pinto, Luísa
Data de Publicação: 2012
Outros Autores: Pinheiro, António Mateus, Morais, Mónica, Bessa, J. M., Sousa, Nuno
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/20448
Resumo: Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple noninvasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders
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spelling Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neuronsGolgi-coxImmunofluorescenceDepressionStressNeuroplasticityNeurogenesisScience & TechnologyCharacterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple noninvasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disordersPortuguese Foundation for Science and Technology (FCT) - PTDC/SAU-NEU/105180/2008, SFRH/BPD/47174/2008)Public Library of ScienceUniversidade do MinhoPinto, LuísaPinheiro, António MateusMorais, MónicaBessa, J. M.Sousa, Nuno20122012-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/20448eng1932-620310.1371/journal.pone.003311422427964http://www.plosone.org/info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T12:01:07Zoai:repositorium.sdum.uminho.pt:1822/20448Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:51:02.789665Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
title Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
spellingShingle Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
Pinto, Luísa
Golgi-cox
Immunofluorescence
Depression
Stress
Neuroplasticity
Neurogenesis
Science & Technology
title_short Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
title_full Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
title_fullStr Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
title_full_unstemmed Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
title_sort Immuno-Golgi as a tool for analyzing neuronal 3D-dendritic structure in phenotypically characterized neurons
author Pinto, Luísa
author_facet Pinto, Luísa
Pinheiro, António Mateus
Morais, Mónica
Bessa, J. M.
Sousa, Nuno
author_role author
author2 Pinheiro, António Mateus
Morais, Mónica
Bessa, J. M.
Sousa, Nuno
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Pinto, Luísa
Pinheiro, António Mateus
Morais, Mónica
Bessa, J. M.
Sousa, Nuno
dc.subject.por.fl_str_mv Golgi-cox
Immunofluorescence
Depression
Stress
Neuroplasticity
Neurogenesis
Science & Technology
topic Golgi-cox
Immunofluorescence
Depression
Stress
Neuroplasticity
Neurogenesis
Science & Technology
description Characterization of neuronal dendritic structure in combination with the determination of specific neuronal phenotype or temporal generation is a challenging task. Here we present a novel method that combines bromodioxyuridine (BrdU) immunohistochemistry with Golgi-impregnation technique; with this simple noninvasive method, we are able to determine the tridimensional structure of dendritic arborization and spine shape of neurons born at a specific time in the hippocampus of adult animals. This analysis is relevant in physiological and pathological conditions in which altered neurogenesis is implicated, such as aging or emotional disorders
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/20448
url http://hdl.handle.net/1822/20448
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 1932-6203
10.1371/journal.pone.0033114
22427964
http://www.plosone.org/
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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