In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient

Bibliographic Details
Main Author: Amorim, Cláudia
Publication Date: 2020
Other Authors: Silvério, Sara Isabel Cruz, Cardoso, B., Alves, J. I., Pereira, M. A., Rodrigues, L. R.
Format: Article
Language: eng
Source: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Download full: http://hdl.handle.net/1822/65610
Summary: Supplementary data to this article can be found online athttps://doi.org/10.1016/j.lwt.2020.109322.
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spelling In vitro fermentation of raffinose to unravel its potential as prebiotic ingredientPrebioticLactuloseRaffinoseHuman fecal inoculain vitro fermentationScience & TechnologySupplementary data to this article can be found online athttps://doi.org/10.1016/j.lwt.2020.109322.Until now the prebiotic potential of pure trisaccharide raffinose on human health assessed through high-throughput sequencing remains poorly investigated. In this work, an in vitro model using human fecal inocula of two healthy volunteers (D1 and D2) was used to study the prebiotic potential of raffinose and compare it with the well-stablished and commercial prebiotic lactulose. The intestinal microbiota showed preference for raffinose as substrate showing the highest consumption value at 48h (96.0±0.9% D1 and 95.3±0.7% D2). The fermentation of raffinose decreased the medium pH, the ammonia concentration and the relative amount of Proteobacteria, while increasing the total production of lactate and short chain fatty acids (129.9±2.6mmol/L D1 and 179.6±0.6mmol/L D2), CO2 (10.8±0.8 mmol/Lmedium D1 and 5.2±0.3 mmol/Lmedium D2) and the relative amount of Bifidobacterium and Lactobacillus. This study suggests that raffinose holds potential functional properties for human health.CA an BBC acknowledge her grants (UMINHO/BPD/4/2019 and SFRH/BD/132324/2017) from Portuguese Foundation for Science andTechnology (FCT). The study receivedfinancial support from FCT underthe scope of the strategic funding of UID/BIO/04469/2020 unit;COMPETE 2020 (POCI-01-0145-FEDER-006684), through nationalfunds and where applicable co-financed by the FEDER, within thePT2020 Partnership Agreement; the Projects FoSynBio (POCI-01-0145-FEDER-029549) and NewFood–Food Technologies Valorization(NORTE-324 01-0246-FEDER-000043). The authors also acknowledgeBioTecNorte operation (NORTE-01-0145-FEDER-000004) funded bythe European Regional Development Fund under the scope ofNorte2020 -Programa Operacional Regional do Norte.info:eu-repo/semantics/publishedVersionElsevierUniversidade do MinhoAmorim, CláudiaSilvério, Sara Isabel CruzCardoso, B.Alves, J. I.Pereira, M. A.Rodrigues, L. R.2020-052020-05-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/65610engAmorim, Cláudia; Silvério, Sara C.; Cardoso, B.; Alves, J. I.; Pereira, M. Alcina; Rodrigues, Lígia R., In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient. LWT-Food Science and Technology, 126(109322), 20200023-64380023-643810.1016/j.lwt.2020.109322https://www.journals.elsevier.com/lwtinfo:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:54:38Zoai:repositorium.sdum.uminho.pt:1822/65610Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:44:07.464970Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient
title In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient
spellingShingle In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient
Amorim, Cláudia
Prebiotic
Lactulose
Raffinose
Human fecal inocula
in vitro fermentation
Science & Technology
title_short In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient
title_full In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient
title_fullStr In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient
title_full_unstemmed In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient
title_sort In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient
author Amorim, Cláudia
author_facet Amorim, Cláudia
Silvério, Sara Isabel Cruz
Cardoso, B.
Alves, J. I.
Pereira, M. A.
Rodrigues, L. R.
author_role author
author2 Silvério, Sara Isabel Cruz
Cardoso, B.
Alves, J. I.
Pereira, M. A.
Rodrigues, L. R.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Amorim, Cláudia
Silvério, Sara Isabel Cruz
Cardoso, B.
Alves, J. I.
Pereira, M. A.
Rodrigues, L. R.
dc.subject.por.fl_str_mv Prebiotic
Lactulose
Raffinose
Human fecal inocula
in vitro fermentation
Science & Technology
topic Prebiotic
Lactulose
Raffinose
Human fecal inocula
in vitro fermentation
Science & Technology
description Supplementary data to this article can be found online athttps://doi.org/10.1016/j.lwt.2020.109322.
publishDate 2020
dc.date.none.fl_str_mv 2020-05
2020-05-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/65610
url http://hdl.handle.net/1822/65610
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Amorim, Cláudia; Silvério, Sara C.; Cardoso, B.; Alves, J. I.; Pereira, M. Alcina; Rodrigues, Lígia R., In vitro fermentation of raffinose to unravel its potential as prebiotic ingredient. LWT-Food Science and Technology, 126(109322), 2020
0023-6438
0023-6438
10.1016/j.lwt.2020.109322
https://www.journals.elsevier.com/lwt
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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