Mining the microbiome of key species from african savanna woodlands
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
| Texto Completo: | http://hdl.handle.net/10362/107798 |
Resumo: | Funding text: This work was supported by funds from Camões, Instituto da Cooperação e da Língua and Fundação para a Ciência e a Tecnologia through the research unit UIDB/00239/2020 (CEF), the PhD grant SFRH/BD/113951/2015 (Ivete Sandra Maquia), and the contribution to the International Rice Research Institute. |
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Mining the microbiome of key species from african savanna woodlandspotential for soil health improvement anwd plant growth promotion16SrRNACombretumFireLimpopo National ParkMopanePlant growth-promoting bacteriaRhizosphereMicrobiologyVirologyMicrobiology (medical)Funding text: This work was supported by funds from Camões, Instituto da Cooperação e da Língua and Fundação para a Ciência e a Tecnologia through the research unit UIDB/00239/2020 (CEF), the PhD grant SFRH/BD/113951/2015 (Ivete Sandra Maquia), and the contribution to the International Rice Research Institute.(1) Aims: Assessing bacterial diversity and plant-growth-promoting functions in the rhizosphere of the native African trees Colophospermum mopane and Combretum apiculatum in three landscapes of the Limpopo National Park (Mozambique), subjected to two fire regimes. (2) Methods: Bacterial communities were identified through Illumina Miseq sequencing of the 16S rRNA gene amplicons, followed by culture dependent methods to isolate plant growth-promoting bacteria (PGPB). Plant growth-promoting traits of the cultivable bacterial fraction were further analyzed. To screen for the presence of nitrogen-fixing bacteria, the promiscuous tropical legume Vigna unguiculata was used as a trap host. The taxonomy of all purified isolates was genetically verified by 16S rRNA gene Sanger sequencing. (3) Results: Bacterial community results indicated that fire did not drive major changes in bacterial abundance. However, culture-dependent methods allowed the differentiation of bacterial communities between the sampled sites, which were particularly enriched in Proteobacteria with a wide range of plant-beneficial traits, such as plant protection, plant nutrition, and plant growth. Bradyrhizobium was the most frequent symbiotic bacteria trapped in cowpea nodules coexisting with other endophytic bacteria. (4) Conclusion: Although the global analysis did not show significant differences between landscapes or sites with different fire regimes, probably due to the fast recovery of bacterial communities, the isolation of PGPB suggests that the rhizosphere bacteria are driven by the plant species, soil type, and fire regime, and are potentially associated with a wide range of agricultural, environmental, and industrial applications. Thus, the rhizosphere of African savannah ecosystems seems to be an untapped source of bacterial species and strains that should be further exploited for bio-based solutions.NOVA School of Business and Economics (NOVA SBE)RUNMaquia, Ivete SandraFareleira, PaulaCastro, Isabel Videira E.Brito, Denise R.A.Soares, RicardoChaúque, AnicetoFerreira-Pinto, M. ManuelaLumini, EricaBerruti, AndreaRibeiro, Natasha S.Marques, IsabelRibeiro-Barros, Ana I.2020-11-25T23:58:56Z2020-092020-09-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article21application/pdfhttp://hdl.handle.net/10362/107798eng2076-2607PURE: 19934200https://doi.org/10.3390/microorganisms8091291info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-03-11T04:52:28Zoai:run.unl.pt:10362/107798Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:41:03.952106Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
| dc.title.none.fl_str_mv |
Mining the microbiome of key species from african savanna woodlands potential for soil health improvement anwd plant growth promotion |
| title |
Mining the microbiome of key species from african savanna woodlands |
| spellingShingle |
Mining the microbiome of key species from african savanna woodlands Maquia, Ivete Sandra 16SrRNA Combretum Fire Limpopo National Park Mopane Plant growth-promoting bacteria Rhizosphere Microbiology Virology Microbiology (medical) |
| title_short |
Mining the microbiome of key species from african savanna woodlands |
| title_full |
Mining the microbiome of key species from african savanna woodlands |
| title_fullStr |
Mining the microbiome of key species from african savanna woodlands |
| title_full_unstemmed |
Mining the microbiome of key species from african savanna woodlands |
| title_sort |
Mining the microbiome of key species from african savanna woodlands |
| author |
Maquia, Ivete Sandra |
| author_facet |
Maquia, Ivete Sandra Fareleira, Paula Castro, Isabel Videira E. Brito, Denise R.A. Soares, Ricardo Chaúque, Aniceto Ferreira-Pinto, M. Manuela Lumini, Erica Berruti, Andrea Ribeiro, Natasha S. Marques, Isabel Ribeiro-Barros, Ana I. |
| author_role |
author |
| author2 |
Fareleira, Paula Castro, Isabel Videira E. Brito, Denise R.A. Soares, Ricardo Chaúque, Aniceto Ferreira-Pinto, M. Manuela Lumini, Erica Berruti, Andrea Ribeiro, Natasha S. Marques, Isabel Ribeiro-Barros, Ana I. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.none.fl_str_mv |
NOVA School of Business and Economics (NOVA SBE) RUN |
| dc.contributor.author.fl_str_mv |
Maquia, Ivete Sandra Fareleira, Paula Castro, Isabel Videira E. Brito, Denise R.A. Soares, Ricardo Chaúque, Aniceto Ferreira-Pinto, M. Manuela Lumini, Erica Berruti, Andrea Ribeiro, Natasha S. Marques, Isabel Ribeiro-Barros, Ana I. |
| dc.subject.por.fl_str_mv |
16SrRNA Combretum Fire Limpopo National Park Mopane Plant growth-promoting bacteria Rhizosphere Microbiology Virology Microbiology (medical) |
| topic |
16SrRNA Combretum Fire Limpopo National Park Mopane Plant growth-promoting bacteria Rhizosphere Microbiology Virology Microbiology (medical) |
| description |
Funding text: This work was supported by funds from Camões, Instituto da Cooperação e da Língua and Fundação para a Ciência e a Tecnologia through the research unit UIDB/00239/2020 (CEF), the PhD grant SFRH/BD/113951/2015 (Ivete Sandra Maquia), and the contribution to the International Rice Research Institute. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-11-25T23:58:56Z 2020-09 2020-09-01T00:00:00Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10362/107798 |
| url |
http://hdl.handle.net/10362/107798 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
2076-2607 PURE: 19934200 https://doi.org/10.3390/microorganisms8091291 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
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openAccess |
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21 application/pdf |
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reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação instacron:RCAAP |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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RCAAP |
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RCAAP |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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