Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cells

Detalhes bibliográficos
Autor(a) principal: Bharmoria, Pankaj
Data de Publicação: 2021
Outros Autores: Bisht, Meena, Gomes, Maria C., Martins, Margarida, Neves, Márcia C., Mano, João F., Bdikin, Igor, Coutinho, João A. P., Ventura, Sónia P. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10773/35552
Resumo: The sustainable cellular delivery of the pleiotropic drug curcumin encounters drawbacks related to its fast autoxidation at the physiological pH, cytotoxicity of delivery vehicles and poor cellular uptake. A biomaterial compatible with curcumin and with the appropriate structure to allow the correct curcumin encapsulation considering its poor solubility in water, while maintaining its stability for a safe release was developed. In this work, the biomaterial developed started by the preparation of an oil-in-water nanoemulsion using with a cytocompatible copolymer (Pluronic F 127) coated with a positively charged protein (gelatin), designed as G-Cur-NE, to mitigate the cytotoxicity issue of curcumin. These G-Cur-NE showed excellent capacity to stabilize curcumin, to increase its bio-accessibility, while allowing to arrest its autoxidation during its successful application as an anticancer agent proved by the disintegration of MDA-MB-231 breast cancer cells as a proof of concept.
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spelling Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cellsAnimalsAntineoplastic agentsBreast neoplasmsCell lineCurcuminDrug carriersDrug delivery systemsDrug stabilityEmulsionsFemaleFibroblastsGelatinHumansMiceNanostructuresOlive OilPoloxamerWaterPhytogenicTumorThe sustainable cellular delivery of the pleiotropic drug curcumin encounters drawbacks related to its fast autoxidation at the physiological pH, cytotoxicity of delivery vehicles and poor cellular uptake. A biomaterial compatible with curcumin and with the appropriate structure to allow the correct curcumin encapsulation considering its poor solubility in water, while maintaining its stability for a safe release was developed. In this work, the biomaterial developed started by the preparation of an oil-in-water nanoemulsion using with a cytocompatible copolymer (Pluronic F 127) coated with a positively charged protein (gelatin), designed as G-Cur-NE, to mitigate the cytotoxicity issue of curcumin. These G-Cur-NE showed excellent capacity to stabilize curcumin, to increase its bio-accessibility, while allowing to arrest its autoxidation during its successful application as an anticancer agent proved by the disintegration of MDA-MB-231 breast cancer cells as a proof of concept.Nature Research2023-01-02T11:09:01Z2021-04-27T00:00:00Z2021-04-27info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10773/35552eng10.1038/s41598-021-88482-3Bharmoria, PankajBisht, MeenaGomes, Maria C.Martins, MargaridaNeves, Márcia C.Mano, João F.Bdikin, IgorCoutinho, João A. P.Ventura, Sónia P. M.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2024-02-22T12:08:18Zoai:ria.ua.pt:10773/35552Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-20T03:06:29.388870Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cells
title Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cells
spellingShingle Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cells
Bharmoria, Pankaj
Animals
Antineoplastic agents
Breast neoplasms
Cell line
Curcumin
Drug carriers
Drug delivery systems
Drug stability
Emulsions
Female
Fibroblasts
Gelatin
Humans
Mice
Nanostructures
Olive Oil
Poloxamer
Water
Phytogenic
Tumor
title_short Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cells
title_full Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cells
title_fullStr Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cells
title_full_unstemmed Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cells
title_sort Protein-olive oil-in-water nanoemulsions as encapsulation materials for curcumin acting as anticancer agent towards MDA-MB-231 cells
author Bharmoria, Pankaj
author_facet Bharmoria, Pankaj
Bisht, Meena
Gomes, Maria C.
Martins, Margarida
Neves, Márcia C.
Mano, João F.
Bdikin, Igor
Coutinho, João A. P.
Ventura, Sónia P. M.
author_role author
author2 Bisht, Meena
Gomes, Maria C.
Martins, Margarida
Neves, Márcia C.
Mano, João F.
Bdikin, Igor
Coutinho, João A. P.
Ventura, Sónia P. M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bharmoria, Pankaj
Bisht, Meena
Gomes, Maria C.
Martins, Margarida
Neves, Márcia C.
Mano, João F.
Bdikin, Igor
Coutinho, João A. P.
Ventura, Sónia P. M.
dc.subject.por.fl_str_mv Animals
Antineoplastic agents
Breast neoplasms
Cell line
Curcumin
Drug carriers
Drug delivery systems
Drug stability
Emulsions
Female
Fibroblasts
Gelatin
Humans
Mice
Nanostructures
Olive Oil
Poloxamer
Water
Phytogenic
Tumor
topic Animals
Antineoplastic agents
Breast neoplasms
Cell line
Curcumin
Drug carriers
Drug delivery systems
Drug stability
Emulsions
Female
Fibroblasts
Gelatin
Humans
Mice
Nanostructures
Olive Oil
Poloxamer
Water
Phytogenic
Tumor
description The sustainable cellular delivery of the pleiotropic drug curcumin encounters drawbacks related to its fast autoxidation at the physiological pH, cytotoxicity of delivery vehicles and poor cellular uptake. A biomaterial compatible with curcumin and with the appropriate structure to allow the correct curcumin encapsulation considering its poor solubility in water, while maintaining its stability for a safe release was developed. In this work, the biomaterial developed started by the preparation of an oil-in-water nanoemulsion using with a cytocompatible copolymer (Pluronic F 127) coated with a positively charged protein (gelatin), designed as G-Cur-NE, to mitigate the cytotoxicity issue of curcumin. These G-Cur-NE showed excellent capacity to stabilize curcumin, to increase its bio-accessibility, while allowing to arrest its autoxidation during its successful application as an anticancer agent proved by the disintegration of MDA-MB-231 breast cancer cells as a proof of concept.
publishDate 2021
dc.date.none.fl_str_mv 2021-04-27T00:00:00Z
2021-04-27
2023-01-02T11:09:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10773/35552
url http://hdl.handle.net/10773/35552
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1038/s41598-021-88482-3
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Research
publisher.none.fl_str_mv Nature Research
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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