Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures

Detalhes bibliográficos
Autor(a) principal: Viegas, Carla
Data de Publicação: 2023
Outros Autores: Simões, Ana Beatriz, Faria, Mariana, Gomes, Bianca, Cervantes, Renata, Dias, Marta, Carolino, Elisabete, Twaruzek, Magdalena, Kosicki, Robert, Viegas, Susana, Caetano, Liliana Aranha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/10400.21/15130
Resumo: Project “Resistant mycobiota and mycotoxigenic profile of tea and medicinal plants” (IPL/2020/TEAResMyc_ESTeSL).
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spelling Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposuresFood contaminationAspergillusCamellia sinensisFood safetyHerbal teaMycotoxinsIPL/2020/TEAResMyc_ESTeSLIPL/2021/ARPTG_ESTeSLProject “Resistant mycobiota and mycotoxigenic profile of tea and medicinal plants” (IPL/2020/TEAResMyc_ESTeSL).Project “Microbiota and azoles fungicides in Portuguese greenhouses: a hotspot for resistance development?” (IPL/2021/ARPTG_ESTeSL).Despite tea beneficial health effects, there is a substantial risk of tea contamination by harmful pathogens and mycotoxins. A total of 40 tea samples (17 green (raw) tea; 13 black (fermented) tea; 10 herbal infusions or white tea) were purchased from different markets located in the Lisbon district in 2020. All products were directly available to consumers either in bulk (13) and or in individual packages (27). Bacterial analysis was performed by inoculating 150 μL of samples extracts in tryptic soy agar (TSA) supplemented with 0.2 % nystatin medium for mesophilic bacteria, and in Violet Red bile agar (VRBA) medium for coliforms (Gram-negative bacteria). Fungal research was performed by spreading 150 μL of samples in malt extract agar (MEA) supplemented with 0.05 % chloramphenicol and in dichloran-glycerol agar (DG18) media. The molecular detection of the Aspergillus sections Fumigati, Nidulantes, Circumdati, and Flavi was carried out by Real-Time PCR (qPCR). Detection of mycotoxins was performed using a high-performance liquid chromatograph (HPLC) with a mass spectrometry detector. Azole resistance screening was achieved following the EUCAST guidelines. The highest counts of total bacteria (TSA) were obtained in green raw tea (81.6 %), while coliform counts (VRBA) were found in samples from black raw tea (96.2 %). The highest fungal counts were obtained in green raw tea (87.7 % MEA; 69.6 % DG18). Aspergillus sp. was the most prevalent genus in all samples on MEA (54.3 %) and on DG18 (56.2 %). In the raw tea 23 of the samples (57.5 %) presented contamination by one to five mycotoxins in the same sample. One Aspergillus section Fumigati isolate from a green tea beverage recovered from itraconazole-Sabouraud dextrose agar (SDA) medium, presented itraconazole and posaconazole E-test MICs above MIC90 values. Our findings open further discussion regarding the One-Health approach and the necessary investment in researching biological hazards and azole resistance associated with the production and consumption of tea (in particular green tea).ElsevierRCIPLViegas, CarlaSimões, Ana BeatrizFaria, MarianaGomes, BiancaCervantes, RenataDias, MartaCarolino, ElisabeteTwaruzek, MagdalenaKosicki, RobertViegas, SusanaCaetano, Liliana Aranha2023-012023-01-01T00:00:00Z2024-12-07T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.21/15130engViegas C, Simões AB, Faria M, Gomes B, Cervantes R, Dias M, Carolino E, Viegas S, Caetano LA, et al. Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures. Int J Food Microbiol. 2023;385:110015.10.1016/j.ijfoodmicro.2022.110015info:eu-repo/semantics/embargoedAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-08-03T10:12:17Zoai:repositorio.ipl.pt:10400.21/15130Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:22:51.266141Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures
title Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures
spellingShingle Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures
Viegas, Carla
Food contamination
Aspergillus
Camellia sinensis
Food safety
Herbal tea
Mycotoxins
IPL/2020/TEAResMyc_ESTeSL
IPL/2021/ARPTG_ESTeSL
title_short Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures
title_full Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures
title_fullStr Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures
title_full_unstemmed Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures
title_sort Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures
author Viegas, Carla
author_facet Viegas, Carla
Simões, Ana Beatriz
Faria, Mariana
Gomes, Bianca
Cervantes, Renata
Dias, Marta
Carolino, Elisabete
Twaruzek, Magdalena
Kosicki, Robert
Viegas, Susana
Caetano, Liliana Aranha
author_role author
author2 Simões, Ana Beatriz
Faria, Mariana
Gomes, Bianca
Cervantes, Renata
Dias, Marta
Carolino, Elisabete
Twaruzek, Magdalena
Kosicki, Robert
Viegas, Susana
Caetano, Liliana Aranha
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv RCIPL
dc.contributor.author.fl_str_mv Viegas, Carla
Simões, Ana Beatriz
Faria, Mariana
Gomes, Bianca
Cervantes, Renata
Dias, Marta
Carolino, Elisabete
Twaruzek, Magdalena
Kosicki, Robert
Viegas, Susana
Caetano, Liliana Aranha
dc.subject.por.fl_str_mv Food contamination
Aspergillus
Camellia sinensis
Food safety
Herbal tea
Mycotoxins
IPL/2020/TEAResMyc_ESTeSL
IPL/2021/ARPTG_ESTeSL
topic Food contamination
Aspergillus
Camellia sinensis
Food safety
Herbal tea
Mycotoxins
IPL/2020/TEAResMyc_ESTeSL
IPL/2021/ARPTG_ESTeSL
description Project “Resistant mycobiota and mycotoxigenic profile of tea and medicinal plants” (IPL/2020/TEAResMyc_ESTeSL).
publishDate 2023
dc.date.none.fl_str_mv 2023-01
2023-01-01T00:00:00Z
2024-12-07T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/10400.21/15130
url http://hdl.handle.net/10400.21/15130
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Viegas C, Simões AB, Faria M, Gomes B, Cervantes R, Dias M, Carolino E, Viegas S, Caetano LA, et al. Tea contamination by mycotoxins and azole-resistant mycobiota: the need of a One Health approach to tackle exposures. Int J Food Microbiol. 2023;385:110015.
10.1016/j.ijfoodmicro.2022.110015
dc.rights.driver.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
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reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
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