Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10400.14/32580 |
Resumo: | Osteoporosis is a public health problem, with bone loss being the main consequence. Hydroxyapatite (HA) has been largely used as a bioceramic to stimulate bone growth. In our work, a cerium-containing HA (Ce-HA) has been proposed and its effects on the antimicrobial and bone-inducing properties were investigated. The synthesis of the materials occurred by the suspension–precipitation method (SPM). The XRD (X-ray Diffraction) confirmed the crystalline phase, and the Rietveld refinement confirmed the crystallization of HA and Ce-HA in a hexagonal crystal structure in agreement with ICSD n◦ 26205. Characterizations by FT-IR (Fourier Transform Infrared Spectroscopy), XPS (X-ray Photoemission Spectroscopy), and FESEM-EDS (Field Emission Scanning Electron Microscope-Energy Dispersive X-ray Spectroscopy) confirmed the presence of cerium (Ce3+ and Ce4+ ). The antibacterial activity of Has was evaluated against Staphylococcus aureus 25,923 and Escherichia coli 25,922 strains, which revealed that the material has antimicrobial properties and the cytotoxicity assay indicated that Ce-containing HA was classified as non-toxic. The effects of Ce-HA on bone repair, after application in bone defects in the tibia of female rats with osteoporosis induced by ovariectomy (OVX), were evaluated. After 15 and 30 days of implantation, the samples were analyzed by Raman, histology and X-ray microtomography. The results showed that the animals that had the induced bone defects filled with the Ce-HA materials had more expressive bone neoformation than the control group. |
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Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomyBone regenerationCeriumHydroxyapatiteOsteoporosisOvariectomyOsteoporosis is a public health problem, with bone loss being the main consequence. Hydroxyapatite (HA) has been largely used as a bioceramic to stimulate bone growth. In our work, a cerium-containing HA (Ce-HA) has been proposed and its effects on the antimicrobial and bone-inducing properties were investigated. The synthesis of the materials occurred by the suspension–precipitation method (SPM). The XRD (X-ray Diffraction) confirmed the crystalline phase, and the Rietveld refinement confirmed the crystallization of HA and Ce-HA in a hexagonal crystal structure in agreement with ICSD n◦ 26205. Characterizations by FT-IR (Fourier Transform Infrared Spectroscopy), XPS (X-ray Photoemission Spectroscopy), and FESEM-EDS (Field Emission Scanning Electron Microscope-Energy Dispersive X-ray Spectroscopy) confirmed the presence of cerium (Ce3+ and Ce4+ ). The antibacterial activity of Has was evaluated against Staphylococcus aureus 25,923 and Escherichia coli 25,922 strains, which revealed that the material has antimicrobial properties and the cytotoxicity assay indicated that Ce-containing HA was classified as non-toxic. The effects of Ce-HA on bone repair, after application in bone defects in the tibia of female rats with osteoporosis induced by ovariectomy (OVX), were evaluated. After 15 and 30 days of implantation, the samples were analyzed by Raman, histology and X-ray microtomography. The results showed that the animals that had the induced bone defects filled with the Ce-HA materials had more expressive bone neoformation than the control group.Veritati - Repositório Institucional da Universidade Católica PortuguesaVieira, EwertonSilva, MarcosMaia-Filho, AntonioFerreira, DanielFiguerêdo-Silva, JoséRovaris, KarlaFialho, Ana CristinaLeite-Oliveira, AnaOliveira, André L. Menezes deFonseca, Maria Gardênnia daOsajima, Josy A.Silva-Filho, Edon C. da2021-04-14T14:11:58Z2021-042021-04-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10400.14/32580eng2075-163X10.3390/min1104037785103490645000643331900001info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-12-19T01:37:11Zoai:repositorio.ucp.pt:10400.14/32580Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:26:19.273469Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy |
title |
Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy |
spellingShingle |
Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy Vieira, Ewerton Bone regeneration Cerium Hydroxyapatite Osteoporosis Ovariectomy |
title_short |
Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy |
title_full |
Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy |
title_fullStr |
Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy |
title_full_unstemmed |
Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy |
title_sort |
Effect of cerium-containing hydroxyapatite in bone repair in female rats with osteoporosis induced by ovariectomy |
author |
Vieira, Ewerton |
author_facet |
Vieira, Ewerton Silva, Marcos Maia-Filho, Antonio Ferreira, Daniel Figuerêdo-Silva, José Rovaris, Karla Fialho, Ana Cristina Leite-Oliveira, Ana Oliveira, André L. Menezes de Fonseca, Maria Gardênnia da Osajima, Josy A. Silva-Filho, Edon C. da |
author_role |
author |
author2 |
Silva, Marcos Maia-Filho, Antonio Ferreira, Daniel Figuerêdo-Silva, José Rovaris, Karla Fialho, Ana Cristina Leite-Oliveira, Ana Oliveira, André L. Menezes de Fonseca, Maria Gardênnia da Osajima, Josy A. Silva-Filho, Edon C. da |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Veritati - Repositório Institucional da Universidade Católica Portuguesa |
dc.contributor.author.fl_str_mv |
Vieira, Ewerton Silva, Marcos Maia-Filho, Antonio Ferreira, Daniel Figuerêdo-Silva, José Rovaris, Karla Fialho, Ana Cristina Leite-Oliveira, Ana Oliveira, André L. Menezes de Fonseca, Maria Gardênnia da Osajima, Josy A. Silva-Filho, Edon C. da |
dc.subject.por.fl_str_mv |
Bone regeneration Cerium Hydroxyapatite Osteoporosis Ovariectomy |
topic |
Bone regeneration Cerium Hydroxyapatite Osteoporosis Ovariectomy |
description |
Osteoporosis is a public health problem, with bone loss being the main consequence. Hydroxyapatite (HA) has been largely used as a bioceramic to stimulate bone growth. In our work, a cerium-containing HA (Ce-HA) has been proposed and its effects on the antimicrobial and bone-inducing properties were investigated. The synthesis of the materials occurred by the suspension–precipitation method (SPM). The XRD (X-ray Diffraction) confirmed the crystalline phase, and the Rietveld refinement confirmed the crystallization of HA and Ce-HA in a hexagonal crystal structure in agreement with ICSD n◦ 26205. Characterizations by FT-IR (Fourier Transform Infrared Spectroscopy), XPS (X-ray Photoemission Spectroscopy), and FESEM-EDS (Field Emission Scanning Electron Microscope-Energy Dispersive X-ray Spectroscopy) confirmed the presence of cerium (Ce3+ and Ce4+ ). The antibacterial activity of Has was evaluated against Staphylococcus aureus 25,923 and Escherichia coli 25,922 strains, which revealed that the material has antimicrobial properties and the cytotoxicity assay indicated that Ce-containing HA was classified as non-toxic. The effects of Ce-HA on bone repair, after application in bone defects in the tibia of female rats with osteoporosis induced by ovariectomy (OVX), were evaluated. After 15 and 30 days of implantation, the samples were analyzed by Raman, histology and X-ray microtomography. The results showed that the animals that had the induced bone defects filled with the Ce-HA materials had more expressive bone neoformation than the control group. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-04-14T14:11:58Z 2021-04 2021-04-01T00:00:00Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10400.14/32580 |
url |
http://hdl.handle.net/10400.14/32580 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
2075-163X 10.3390/min11040377 85103490645 000643331900001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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