Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonance
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
Texto Completo: | http://hdl.handle.net/10316/8095 https://doi.org/10.1002/mrm.20496 |
Resumo: | A recently introduced tracer, [3,4-13C2] glucose, was compared to the widely used tracer, [6,6-2H2]glucose, for measurement of whole-body glucose turnover. The rate of glucose production (GP) was measured in rats after primed infusions of [3,4-13C2]glucose, [6,6-2H2]glucose, or both tracers simultaneously followed by a constant infusion of tracer(s) over 90 min. Blood glucose was purified and converted into monoacetone glucose for analysis by 13C NMR (for [3,4-13C2]glucose) or 1H and 2H NMR (for [6,6-2H2]glucose). The values of GP measured during infusion of each single tracer were not significantly different. In rats infused with both tracers simultaneously, GP was identical as reported by each tracer, 42 ± 4 mumol/kg/min. Since 2H and 13C enrichment in glucose is typically much less than 2% for in vivo studies, [3,4-13C2]glucose does not interfere with measurements of 13C or 2H enrichment patterns and therefore is valuable when multiple metabolic pathways are being evaluated simultaneously. Magn Reson Med 53:1479-1483, 2005. © 2005 Wiley-Liss, Inc. |
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Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonanceA recently introduced tracer, [3,4-13C2] glucose, was compared to the widely used tracer, [6,6-2H2]glucose, for measurement of whole-body glucose turnover. The rate of glucose production (GP) was measured in rats after primed infusions of [3,4-13C2]glucose, [6,6-2H2]glucose, or both tracers simultaneously followed by a constant infusion of tracer(s) over 90 min. Blood glucose was purified and converted into monoacetone glucose for analysis by 13C NMR (for [3,4-13C2]glucose) or 1H and 2H NMR (for [6,6-2H2]glucose). The values of GP measured during infusion of each single tracer were not significantly different. In rats infused with both tracers simultaneously, GP was identical as reported by each tracer, 42 ± 4 mumol/kg/min. Since 2H and 13C enrichment in glucose is typically much less than 2% for in vivo studies, [3,4-13C2]glucose does not interfere with measurements of 13C or 2H enrichment patterns and therefore is valuable when multiple metabolic pathways are being evaluated simultaneously. Magn Reson Med 53:1479-1483, 2005. © 2005 Wiley-Liss, Inc.2005info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://hdl.handle.net/10316/8095http://hdl.handle.net/10316/8095https://doi.org/10.1002/mrm.20496engMagnetic Resonance in Medicine. 53:6 (2005) 1479-1483Jin, Eunsook S.Jones, John G.Burgess, Shawn C.Merritt, Matthew E.Sherry, A. DeanMalloy, Craig R.info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2021-10-26T13:39:34Zoai:estudogeral.uc.pt:10316/8095Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T20:55:43.973113Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse |
dc.title.none.fl_str_mv |
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonance |
title |
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonance |
spellingShingle |
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonance Jin, Eunsook S. |
title_short |
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonance |
title_full |
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonance |
title_fullStr |
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonance |
title_full_unstemmed |
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonance |
title_sort |
Comparison of [3,4-13C2] glucose as a tracer for glucose turnover by nuclear magnetic resonance |
author |
Jin, Eunsook S. |
author_facet |
Jin, Eunsook S. Jones, John G. Burgess, Shawn C. Merritt, Matthew E. Sherry, A. Dean Malloy, Craig R. |
author_role |
author |
author2 |
Jones, John G. Burgess, Shawn C. Merritt, Matthew E. Sherry, A. Dean Malloy, Craig R. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Jin, Eunsook S. Jones, John G. Burgess, Shawn C. Merritt, Matthew E. Sherry, A. Dean Malloy, Craig R. |
description |
A recently introduced tracer, [3,4-13C2] glucose, was compared to the widely used tracer, [6,6-2H2]glucose, for measurement of whole-body glucose turnover. The rate of glucose production (GP) was measured in rats after primed infusions of [3,4-13C2]glucose, [6,6-2H2]glucose, or both tracers simultaneously followed by a constant infusion of tracer(s) over 90 min. Blood glucose was purified and converted into monoacetone glucose for analysis by 13C NMR (for [3,4-13C2]glucose) or 1H and 2H NMR (for [6,6-2H2]glucose). The values of GP measured during infusion of each single tracer were not significantly different. In rats infused with both tracers simultaneously, GP was identical as reported by each tracer, 42 ± 4 mumol/kg/min. Since 2H and 13C enrichment in glucose is typically much less than 2% for in vivo studies, [3,4-13C2]glucose does not interfere with measurements of 13C or 2H enrichment patterns and therefore is valuable when multiple metabolic pathways are being evaluated simultaneously. Magn Reson Med 53:1479-1483, 2005. © 2005 Wiley-Liss, Inc. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10316/8095 http://hdl.handle.net/10316/8095 https://doi.org/10.1002/mrm.20496 |
url |
http://hdl.handle.net/10316/8095 https://doi.org/10.1002/mrm.20496 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Magnetic Resonance in Medicine. 53:6 (2005) 1479-1483 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
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Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) |
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Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação |
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