Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species

Detalhes bibliográficos
Autor(a) principal: Santos, C.
Data de Publicação: 2011
Outros Autores: Lima, Nelson, Sampaio, Paula, Pais, Célia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
Texto Completo: http://hdl.handle.net/1822/16639
Resumo: Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C. parapsilosis, C. orthopsilosis, and C. metapsilsosis, and very closely related species like C. glabrata and C. bracarensis, and C. albicans and C. dubliniensis could be clearly separated. MALDI-TOF-ICMS stands out as a promising tool for the rapid detection of emerging pathogens.
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spelling Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida speciesCandidaC. parapsilosis complexMALDI-TOF ICMSScience & TechnologyMatrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C. parapsilosis, C. orthopsilosis, and C. metapsilsosis, and very closely related species like C. glabrata and C. bracarensis, and C. albicans and C. dubliniensis could be clearly separated. MALDI-TOF-ICMS stands out as a promising tool for the rapid detection of emerging pathogens.Research leading to these results received funding from the European Community's Seventh Framework Program (FP7 2007-2013), Research Infrastructures Action, under grant agreement no. FP7-228310 (EMbaRC project).ElsevierUniversidade do MinhoSantos, C.Lima, NelsonSampaio, PaulaPais, Célia20112011-01-01T00:00:00Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/1822/16639eng0732-889310.1016/j.diagmicrobio.2011.07.00221855250info:eu-repo/semantics/openAccessreponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãoinstacron:RCAAP2023-07-21T11:54:29Zoai:repositorium.sdum.uminho.pt:1822/16639Portal AgregadorONGhttps://www.rcaap.pt/oai/openaireopendoar:71602024-03-19T18:43:58.236363Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informaçãofalse
dc.title.none.fl_str_mv Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species
title Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species
spellingShingle Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species
Santos, C.
Candida
C. parapsilosis complex
MALDI-TOF ICMS
Science & Technology
title_short Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species
title_full Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species
title_fullStr Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species
title_full_unstemmed Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species
title_sort Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry to detect emerging pathogenic Candida species
author Santos, C.
author_facet Santos, C.
Lima, Nelson
Sampaio, Paula
Pais, Célia
author_role author
author2 Lima, Nelson
Sampaio, Paula
Pais, Célia
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade do Minho
dc.contributor.author.fl_str_mv Santos, C.
Lima, Nelson
Sampaio, Paula
Pais, Célia
dc.subject.por.fl_str_mv Candida
C. parapsilosis complex
MALDI-TOF ICMS
Science & Technology
topic Candida
C. parapsilosis complex
MALDI-TOF ICMS
Science & Technology
description Matrix-assisted laser desorption/ionization time-of-flight intact cell mass spectrometry (MALDI-TOF-ICMS) was used to differentiate pathogenic Candida species, difficult to identify by traditional methods such as growth and biochemical reactions. Results showed that species complexes like C. parapsilosis, C. orthopsilosis, and C. metapsilsosis, and very closely related species like C. glabrata and C. bracarensis, and C. albicans and C. dubliniensis could be clearly separated. MALDI-TOF-ICMS stands out as a promising tool for the rapid detection of emerging pathogens.
publishDate 2011
dc.date.none.fl_str_mv 2011
2011-01-01T00:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://hdl.handle.net/1822/16639
url http://hdl.handle.net/1822/16639
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 0732-8893
10.1016/j.diagmicrobio.2011.07.002
21855250
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
instname:Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron:RCAAP
instname_str Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
instacron_str RCAAP
institution RCAAP
reponame_str Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
collection Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos)
repository.name.fl_str_mv Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) - Agência para a Sociedade do Conhecimento (UMIC) - FCT - Sociedade da Informação
repository.mail.fl_str_mv
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