Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model
Main Author: | |
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Publication Date: | 2022 |
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Format: | Article |
Language: | eng |
Source: | Food Science and Technology (Campinas) |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101245 |
Summary: | Abstract In this study, the exopolysaccharides (EPSs)-producing L. plantarum YW11 was initially evaluated for its tolerance to simulated artificial gastric juice and bile salts, and cell surface hydrophobicity. Subsequent study using a HT-29 tumor-burdened nude mouse model showed that the EPS had potent immunomodulatory and antitumor activities. Administration of the EPS had no influence on body weight of the mice, and it could significantly prevent HT-29 tumor cells induced acute liver and kidney damages. The purified EPS at a dose of 5 mg/mL was able to enhance effectively the killing activities of splenic natural killer cells and cytotoxic T lymphocyte, and to stimulate the secretion of serum cytokines IL-2 and TNF-α. As evidenced by a cell culture assay, the EPS exhibited obvious inhibition on growth of colon cancer HT-29 cells treated with 600 μg/mL of the EPS for 72 h with an inhibition rate of 58.02%. Administration of the EPS obviously inhibited growth of HT-29 xenograft tumor to an extent near that of the positive control (5-Fu, 25 mg/kg bw). Therefore, the results of this study indicated the important roles of the EPS in the potential probiotic functions of the strain. |
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Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse modelexopolysaccharideLactobacillus plantarumImmunomodulatoryantitumor, HT-29 cellsAbstract In this study, the exopolysaccharides (EPSs)-producing L. plantarum YW11 was initially evaluated for its tolerance to simulated artificial gastric juice and bile salts, and cell surface hydrophobicity. Subsequent study using a HT-29 tumor-burdened nude mouse model showed that the EPS had potent immunomodulatory and antitumor activities. Administration of the EPS had no influence on body weight of the mice, and it could significantly prevent HT-29 tumor cells induced acute liver and kidney damages. The purified EPS at a dose of 5 mg/mL was able to enhance effectively the killing activities of splenic natural killer cells and cytotoxic T lymphocyte, and to stimulate the secretion of serum cytokines IL-2 and TNF-α. As evidenced by a cell culture assay, the EPS exhibited obvious inhibition on growth of colon cancer HT-29 cells treated with 600 μg/mL of the EPS for 72 h with an inhibition rate of 58.02%. Administration of the EPS obviously inhibited growth of HT-29 xenograft tumor to an extent near that of the positive control (5-Fu, 25 mg/kg bw). Therefore, the results of this study indicated the important roles of the EPS in the potential probiotic functions of the strain.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101245Food Science and Technology v.42 2022reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/fst.57822info:eu-repo/semantics/openAccessZHANG,MinWANG,JiYANG,Zhennaieng2022-07-14T00:00:00Zoai:scielo:S0101-20612022000101245Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2022-07-14T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model |
title |
Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model |
spellingShingle |
Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model ZHANG,Min exopolysaccharide Lactobacillus plantarum Immunomodulatory antitumor, HT-29 cells |
title_short |
Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model |
title_full |
Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model |
title_fullStr |
Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model |
title_full_unstemmed |
Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model |
title_sort |
Immunomodulatory and antitumor activities of the exopolysaccharide produced by potential probiotic Lactobacillus plantarum YW11 in a HT-29 tumor-burdened nude mouse model |
author |
ZHANG,Min |
author_facet |
ZHANG,Min WANG,Ji YANG,Zhennai |
author_role |
author |
author2 |
WANG,Ji YANG,Zhennai |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
ZHANG,Min WANG,Ji YANG,Zhennai |
dc.subject.por.fl_str_mv |
exopolysaccharide Lactobacillus plantarum Immunomodulatory antitumor, HT-29 cells |
topic |
exopolysaccharide Lactobacillus plantarum Immunomodulatory antitumor, HT-29 cells |
description |
Abstract In this study, the exopolysaccharides (EPSs)-producing L. plantarum YW11 was initially evaluated for its tolerance to simulated artificial gastric juice and bile salts, and cell surface hydrophobicity. Subsequent study using a HT-29 tumor-burdened nude mouse model showed that the EPS had potent immunomodulatory and antitumor activities. Administration of the EPS had no influence on body weight of the mice, and it could significantly prevent HT-29 tumor cells induced acute liver and kidney damages. The purified EPS at a dose of 5 mg/mL was able to enhance effectively the killing activities of splenic natural killer cells and cytotoxic T lymphocyte, and to stimulate the secretion of serum cytokines IL-2 and TNF-α. As evidenced by a cell culture assay, the EPS exhibited obvious inhibition on growth of colon cancer HT-29 cells treated with 600 μg/mL of the EPS for 72 h with an inhibition rate of 58.02%. Administration of the EPS obviously inhibited growth of HT-29 xenograft tumor to an extent near that of the positive control (5-Fu, 25 mg/kg bw). Therefore, the results of this study indicated the important roles of the EPS in the potential probiotic functions of the strain. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101245 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612022000101245 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/fst.57822 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.42 2022 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
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Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
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SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
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Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
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