ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSIS
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Food Science and Technology (Campinas) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612001000300016 |
Resumo: | The importance of starch for the food industry makes it necessary to develop new, fast, economic and accurate methodologies for its quantification. In the present paper starch hydrolysis using commercial enzymes of industrial grade are studied aiming to develop an easy and cheap analysis, available to a greater number of industries and technicians. The proposed method is simple, divided in a first step where soluble sugars are eliminated from the samples by using dialysis, followed by starch hydrolysis of the retained fraction with a thermoresistent bacterial alfa-amylase (Termamyl 120L®) and an amyloglucosidase (AMG 300L®). The hydrolysis conditions were those suggested by the enzyme producer. After the hydrolysis step the material was dialysed again for the extraction of glucose that was quantified by the glucose-oxidase colorimetric reactant. The results allowed the construction of calibration equations for starch determination on the analyzed samples. These samples were produced on a laboratory scale and native and acid-modified corn starches were added in known concentrations. By considering the final dilutions employed for glucose determination on the samples, it was possible to confirm that they were identical to that of the glucose-oxidase reactant calibration. |
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Food Science and Technology (Campinas) |
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ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSISstarch quantificationdoce de leitedialysisThe importance of starch for the food industry makes it necessary to develop new, fast, economic and accurate methodologies for its quantification. In the present paper starch hydrolysis using commercial enzymes of industrial grade are studied aiming to develop an easy and cheap analysis, available to a greater number of industries and technicians. The proposed method is simple, divided in a first step where soluble sugars are eliminated from the samples by using dialysis, followed by starch hydrolysis of the retained fraction with a thermoresistent bacterial alfa-amylase (Termamyl 120L®) and an amyloglucosidase (AMG 300L®). The hydrolysis conditions were those suggested by the enzyme producer. After the hydrolysis step the material was dialysed again for the extraction of glucose that was quantified by the glucose-oxidase colorimetric reactant. The results allowed the construction of calibration equations for starch determination on the analyzed samples. These samples were produced on a laboratory scale and native and acid-modified corn starches were added in known concentrations. By considering the final dilutions employed for glucose determination on the samples, it was possible to confirm that they were identical to that of the glucose-oxidase reactant calibration.Sociedade Brasileira de Ciência e Tecnologia de Alimentos2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612001000300016Food Science and Technology v.21 n.3 2001reponame:Food Science and Technology (Campinas)instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)instacron:SBCTA10.1590/S0101-20612001000300016info:eu-repo/semantics/openAccessDEMIATE,Ivo MotimKONKEL,Francisco EnéiasPEDROSO,Ricardo Alexandreeng2002-05-27T00:00:00Zoai:scielo:S0101-20612001000300016Revistahttp://www.scielo.br/ctaONGhttps://old.scielo.br/oai/scielo-oai.php||revista@sbcta.org.br1678-457X0101-2061opendoar:2002-05-27T00:00Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA)false |
dc.title.none.fl_str_mv |
ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSIS |
title |
ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSIS |
spellingShingle |
ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSIS DEMIATE,Ivo Motim starch quantification doce de leite dialysis |
title_short |
ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSIS |
title_full |
ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSIS |
title_fullStr |
ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSIS |
title_full_unstemmed |
ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSIS |
title_sort |
ENZYMATIC DETERMINATION OF STARCH IN DOCE DE LEITE USING DIALYSIS |
author |
DEMIATE,Ivo Motim |
author_facet |
DEMIATE,Ivo Motim KONKEL,Francisco Enéias PEDROSO,Ricardo Alexandre |
author_role |
author |
author2 |
KONKEL,Francisco Enéias PEDROSO,Ricardo Alexandre |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
DEMIATE,Ivo Motim KONKEL,Francisco Enéias PEDROSO,Ricardo Alexandre |
dc.subject.por.fl_str_mv |
starch quantification doce de leite dialysis |
topic |
starch quantification doce de leite dialysis |
description |
The importance of starch for the food industry makes it necessary to develop new, fast, economic and accurate methodologies for its quantification. In the present paper starch hydrolysis using commercial enzymes of industrial grade are studied aiming to develop an easy and cheap analysis, available to a greater number of industries and technicians. The proposed method is simple, divided in a first step where soluble sugars are eliminated from the samples by using dialysis, followed by starch hydrolysis of the retained fraction with a thermoresistent bacterial alfa-amylase (Termamyl 120L®) and an amyloglucosidase (AMG 300L®). The hydrolysis conditions were those suggested by the enzyme producer. After the hydrolysis step the material was dialysed again for the extraction of glucose that was quantified by the glucose-oxidase colorimetric reactant. The results allowed the construction of calibration equations for starch determination on the analyzed samples. These samples were produced on a laboratory scale and native and acid-modified corn starches were added in known concentrations. By considering the final dilutions employed for glucose determination on the samples, it was possible to confirm that they were identical to that of the glucose-oxidase reactant calibration. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612001000300016 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612001000300016 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0101-20612001000300016 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
publisher.none.fl_str_mv |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos |
dc.source.none.fl_str_mv |
Food Science and Technology v.21 n.3 2001 reponame:Food Science and Technology (Campinas) instname:Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) instacron:SBCTA |
instname_str |
Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
instacron_str |
SBCTA |
institution |
SBCTA |
reponame_str |
Food Science and Technology (Campinas) |
collection |
Food Science and Technology (Campinas) |
repository.name.fl_str_mv |
Food Science and Technology (Campinas) - Sociedade Brasileira de Ciência e Tecnologia de Alimentos (SBCTA) |
repository.mail.fl_str_mv |
||revista@sbcta.org.br |
_version_ |
1752126310597001216 |