Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients

Detalhes bibliográficos
Autor(a) principal: Chomiski,Verônica
Data de Publicação: 2016
Outros Autores: Gragnani,Alfredo, Bonucci,Jéssica, Correa,Silvana Aparecida Alves, Noronha,Samuel Marcos Ribeiro de, Ferreira,Lydia Masako
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Acta Cirúrgica Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016000800505
Resumo: ABSTRACT PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC. RESULTS: Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes. CONCLUSION: There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.
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spelling Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patientsBurnsGene ExpressionWound HealingKeratinocytes.ABSTRACT PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC. RESULTS: Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes. CONCLUSION: There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2016-08-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016000800505Acta Cirúrgica Brasileira v.31 n.8 2016reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/S0102-865020160080000002info:eu-repo/semantics/openAccessChomiski,VerônicaGragnani,AlfredoBonucci,JéssicaCorrea,Silvana Aparecida AlvesNoronha,Samuel Marcos Ribeiro deFerreira,Lydia Masakoeng2016-08-24T00:00:00Zoai:scielo:S0102-86502016000800505Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2016-08-24T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false
dc.title.none.fl_str_mv Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients
title Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients
spellingShingle Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients
Chomiski,Verônica
Burns
Gene Expression
Wound Healing
Keratinocytes.
title_short Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients
title_full Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients
title_fullStr Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients
title_full_unstemmed Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients
title_sort Keratinocyte growth factor and the expression of wound-healing-related genes in primary human keratinocytes from burn patients
author Chomiski,Verônica
author_facet Chomiski,Verônica
Gragnani,Alfredo
Bonucci,Jéssica
Correa,Silvana Aparecida Alves
Noronha,Samuel Marcos Ribeiro de
Ferreira,Lydia Masako
author_role author
author2 Gragnani,Alfredo
Bonucci,Jéssica
Correa,Silvana Aparecida Alves
Noronha,Samuel Marcos Ribeiro de
Ferreira,Lydia Masako
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Chomiski,Verônica
Gragnani,Alfredo
Bonucci,Jéssica
Correa,Silvana Aparecida Alves
Noronha,Samuel Marcos Ribeiro de
Ferreira,Lydia Masako
dc.subject.por.fl_str_mv Burns
Gene Expression
Wound Healing
Keratinocytes.
topic Burns
Gene Expression
Wound Healing
Keratinocytes.
description ABSTRACT PURPOSE: To evaluate the effect of keratinocyte growth factor (KGF) treatment on the expression of wound-healing-related genes in cultured keratinocytes from burn patients. METHODS: Keratinocytes were cultured and divided into 4 groups (n=4 in each group): TKB (KGF-treated keratinocytes from burn patients), UKB (untreated keratinocytes from burn patients), TKC (KGF-treated keratinocytes from controls), and UKC (untreated keratinocytes from controls). Gene expression analysis using quantitative polymerase chain reaction (qPCR) array was performed to compare (1) TKC versus UKC, (2) UKB versus UKC, (3) TKB versus UKC, (4) TKB versus UKB, (5) TKB versus TKC, and (6) UKB versus TKC. RESULTS: Comparison 1 showed one down-regulated and one up-regulated gene; comparisons 2 and 3 resulted in the same five down-regulated genes; comparison 4 had no significant difference in relative gene expression; comparison 5 showed 26 down-regulated and 7 up-regulated genes; and comparison 6 showed 25 down-regulated and 11 up-regulated genes. CONCLUSION: There was no differential expression of wound-healing-related genes in cultured primary keratinocytes from burn patients treated with keratinocyte growth factor.
publishDate 2016
dc.date.none.fl_str_mv 2016-08-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016000800505
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502016000800505
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0102-865020160080000002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
publisher.none.fl_str_mv Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
dc.source.none.fl_str_mv Acta Cirúrgica Brasileira v.31 n.8 2016
reponame:Acta Cirúrgica Brasileira (Online)
instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron:SBDPC
instname_str Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
instacron_str SBDPC
institution SBDPC
reponame_str Acta Cirúrgica Brasileira (Online)
collection Acta Cirúrgica Brasileira (Online)
repository.name.fl_str_mv Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)
repository.mail.fl_str_mv ||sgolden@terra.com.br
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