Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Acta Cirúrgica Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000900201 |
Resumo: | ABSTRACT Purpose: To investigate the active ingredients of walnut ointment (WO) and its mechanism in repairing wounds. Methods: The ingredients of WO were detected by gas chromatography–mass spectrometry. The effect of linoleic acid (LA) was tested by in vitro Alamar Blue (AB) reagent. Image J software, histological and immunohistochemical analysis were used to confirm the healing effect of LA in the porcine skin model. The animals were euthanized after the experiment by injection of pentobarbital sodium. Results: LA, 24% in WO, promotes keratinocytes and fibroblasts proliferation, which were 50.09% and 15.07% respectively higher than control (p < 0.05). The healing rate of the LA group (96.02% ± 2%, 98.58% ± 0.78%) was higher than the saline group (82.11% ± 3.37%, 88.72% ± 1.73%) at week 3 and week 4 (p < 0.05). The epidermal thickness of the LA was 0.16 ± 0.04 mm greater and the expression of the P63 and CK10 proteins was stronger in the LA group than the control (p < 0.05). Conclusions: LA, which is the main components in WO can promote full-thickness burning wounds (FBWs) by stimulating cell proliferation and differentiation. |
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Walnut ointment promotes full-thickness burning wound healing: role of linoleic acidBurnsSkin Wound HealingWalnut OintmentLinoleic AcidKeratinocytesFibroblastsABSTRACT Purpose: To investigate the active ingredients of walnut ointment (WO) and its mechanism in repairing wounds. Methods: The ingredients of WO were detected by gas chromatography–mass spectrometry. The effect of linoleic acid (LA) was tested by in vitro Alamar Blue (AB) reagent. Image J software, histological and immunohistochemical analysis were used to confirm the healing effect of LA in the porcine skin model. The animals were euthanized after the experiment by injection of pentobarbital sodium. Results: LA, 24% in WO, promotes keratinocytes and fibroblasts proliferation, which were 50.09% and 15.07% respectively higher than control (p < 0.05). The healing rate of the LA group (96.02% ± 2%, 98.58% ± 0.78%) was higher than the saline group (82.11% ± 3.37%, 88.72% ± 1.73%) at week 3 and week 4 (p < 0.05). The epidermal thickness of the LA was 0.16 ± 0.04 mm greater and the expression of the P63 and CK10 proteins was stronger in the LA group than the control (p < 0.05). Conclusions: LA, which is the main components in WO can promote full-thickness burning wounds (FBWs) by stimulating cell proliferation and differentiation.Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000900201Acta Cirúrgica Brasileira v.37 n.9 2022reponame:Acta Cirúrgica Brasileira (Online)instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)instacron:SBDPC10.1590/acb370902info:eu-repo/semantics/openAccessZhao,DanXiao,JinliQiang,LijuanDeng,XingwangAn,JingjingZhang,QingZhao,FangMa,JiaxiangFang,ChaoGuan,GuangyuWu,YinshengXie,Yaneng2022-11-24T00:00:00Zoai:scielo:S0102-86502022000900201Revistahttps://www.bvs-vet.org.br/vetindex/periodicos/acta-cirurgica-brasileira/https://old.scielo.br/oai/scielo-oai.php||sgolden@terra.com.br0102-86501678-2674opendoar:2022-11-24T00:00Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC)false |
dc.title.none.fl_str_mv |
Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title |
Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
spellingShingle |
Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid Zhao,Dan Burns Skin Wound Healing Walnut Ointment Linoleic Acid Keratinocytes Fibroblasts |
title_short |
Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title_full |
Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title_fullStr |
Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title_full_unstemmed |
Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
title_sort |
Walnut ointment promotes full-thickness burning wound healing: role of linoleic acid |
author |
Zhao,Dan |
author_facet |
Zhao,Dan Xiao,Jinli Qiang,Lijuan Deng,Xingwang An,Jingjing Zhang,Qing Zhao,Fang Ma,Jiaxiang Fang,Chao Guan,Guangyu Wu,Yinsheng Xie,Yan |
author_role |
author |
author2 |
Xiao,Jinli Qiang,Lijuan Deng,Xingwang An,Jingjing Zhang,Qing Zhao,Fang Ma,Jiaxiang Fang,Chao Guan,Guangyu Wu,Yinsheng Xie,Yan |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Zhao,Dan Xiao,Jinli Qiang,Lijuan Deng,Xingwang An,Jingjing Zhang,Qing Zhao,Fang Ma,Jiaxiang Fang,Chao Guan,Guangyu Wu,Yinsheng Xie,Yan |
dc.subject.por.fl_str_mv |
Burns Skin Wound Healing Walnut Ointment Linoleic Acid Keratinocytes Fibroblasts |
topic |
Burns Skin Wound Healing Walnut Ointment Linoleic Acid Keratinocytes Fibroblasts |
description |
ABSTRACT Purpose: To investigate the active ingredients of walnut ointment (WO) and its mechanism in repairing wounds. Methods: The ingredients of WO were detected by gas chromatography–mass spectrometry. The effect of linoleic acid (LA) was tested by in vitro Alamar Blue (AB) reagent. Image J software, histological and immunohistochemical analysis were used to confirm the healing effect of LA in the porcine skin model. The animals were euthanized after the experiment by injection of pentobarbital sodium. Results: LA, 24% in WO, promotes keratinocytes and fibroblasts proliferation, which were 50.09% and 15.07% respectively higher than control (p < 0.05). The healing rate of the LA group (96.02% ± 2%, 98.58% ± 0.78%) was higher than the saline group (82.11% ± 3.37%, 88.72% ± 1.73%) at week 3 and week 4 (p < 0.05). The epidermal thickness of the LA was 0.16 ± 0.04 mm greater and the expression of the P63 and CK10 proteins was stronger in the LA group than the control (p < 0.05). Conclusions: LA, which is the main components in WO can promote full-thickness burning wounds (FBWs) by stimulating cell proliferation and differentiation. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000900201 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502022000900201 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/acb370902 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
publisher.none.fl_str_mv |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
dc.source.none.fl_str_mv |
Acta Cirúrgica Brasileira v.37 n.9 2022 reponame:Acta Cirúrgica Brasileira (Online) instname:Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) instacron:SBDPC |
instname_str |
Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
instacron_str |
SBDPC |
institution |
SBDPC |
reponame_str |
Acta Cirúrgica Brasileira (Online) |
collection |
Acta Cirúrgica Brasileira (Online) |
repository.name.fl_str_mv |
Acta Cirúrgica Brasileira (Online) - Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia (SBDPC) |
repository.mail.fl_str_mv |
||sgolden@terra.com.br |
_version_ |
1752126446584725504 |