Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Revista Brasileira de Farmacognosia (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000200003 |
Resumo: | In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G)K. |
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Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolitesantimicrobialbiological screeningcytotoxicmarine-derived fungiIn the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G)K.Sociedade Brasileira de Farmacognosia2012-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000200003Revista Brasileira de Farmacognosia v.22 n.2 2012reponame:Revista Brasileira de Farmacognosia (Online)instname:Sociedade Brasileira de Farmacognosia (SBFgnosia)instacron:SBFGNOSIA10.1590/S0102-695X2011005000222info:eu-repo/semantics/openAccessKossuga,Miriam H.Romminger,StelamarXavier,CamilaMilanetto,Marília C.Valle,Milene Z. doPimenta,Eli F.Morais,Raquel P.Carvalho,Erica deMizuno,Carolina M.Coradello,Luís Fernando C.Barroso,Vinícius de M.Vacondio,BrunaJavaroti,Darci C. D.Seleghim,Mirna H. R.Cavalcanti,Bruno C.Pessoa,ClaudiaMoraes,Manoel O.Lima,Bruna A.Gonçalves,ReginaldoBonugli-Santos,Rafaella C.Sette,Lara D.Berlinck,Roberto G. S.eng2012-05-08T00:00:00Zoai:scielo:S0102-695X2012000200003Revistahttp://www.sbfgnosia.org.br/revista/https://old.scielo.br/oai/scielo-oai.phprbgnosia@ltf.ufpb.br1981-528X0102-695Xopendoar:2012-05-08T00:00Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia)false |
dc.title.none.fl_str_mv |
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites |
title |
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites |
spellingShingle |
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites Kossuga,Miriam H. antimicrobial biological screening cytotoxic marine-derived fungi |
title_short |
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites |
title_full |
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites |
title_fullStr |
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites |
title_full_unstemmed |
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites |
title_sort |
Evaluating methods for the isolation of marine-derived fungal strains and production of bioactive secondary metabolites |
author |
Kossuga,Miriam H. |
author_facet |
Kossuga,Miriam H. Romminger,Stelamar Xavier,Camila Milanetto,Marília C. Valle,Milene Z. do Pimenta,Eli F. Morais,Raquel P. Carvalho,Erica de Mizuno,Carolina M. Coradello,Luís Fernando C. Barroso,Vinícius de M. Vacondio,Bruna Javaroti,Darci C. D. Seleghim,Mirna H. R. Cavalcanti,Bruno C. Pessoa,Claudia Moraes,Manoel O. Lima,Bruna A. Gonçalves,Reginaldo Bonugli-Santos,Rafaella C. Sette,Lara D. Berlinck,Roberto G. S. |
author_role |
author |
author2 |
Romminger,Stelamar Xavier,Camila Milanetto,Marília C. Valle,Milene Z. do Pimenta,Eli F. Morais,Raquel P. Carvalho,Erica de Mizuno,Carolina M. Coradello,Luís Fernando C. Barroso,Vinícius de M. Vacondio,Bruna Javaroti,Darci C. D. Seleghim,Mirna H. R. Cavalcanti,Bruno C. Pessoa,Claudia Moraes,Manoel O. Lima,Bruna A. Gonçalves,Reginaldo Bonugli-Santos,Rafaella C. Sette,Lara D. Berlinck,Roberto G. S. |
author2_role |
author author author author author author author author author author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Kossuga,Miriam H. Romminger,Stelamar Xavier,Camila Milanetto,Marília C. Valle,Milene Z. do Pimenta,Eli F. Morais,Raquel P. Carvalho,Erica de Mizuno,Carolina M. Coradello,Luís Fernando C. Barroso,Vinícius de M. Vacondio,Bruna Javaroti,Darci C. D. Seleghim,Mirna H. R. Cavalcanti,Bruno C. Pessoa,Claudia Moraes,Manoel O. Lima,Bruna A. Gonçalves,Reginaldo Bonugli-Santos,Rafaella C. Sette,Lara D. Berlinck,Roberto G. S. |
dc.subject.por.fl_str_mv |
antimicrobial biological screening cytotoxic marine-derived fungi |
topic |
antimicrobial biological screening cytotoxic marine-derived fungi |
description |
In the present investigation we evaluate methods for the isolation and growth of marine-derived fungal strains in artificial media for the production of secondary metabolites. Inoculation of marine macroorganisms fragments in Petri dishes proved to be the most convenient procedure for the isolation of the largest number of strains. Among the growth media used, 3% malt extract showed the best result for strains isolation and growth, and yielded the largest number of strains from marine macroorganisms. The percentage of strains isolated using each of the growth media which yielded cytotoxic and/or antibiotic extracts was in the range of 23-35%, regardless of the growth media used. Further investigation of extracts obtained from different marine-derived fungal strains yielded several bioactive secondary metabolites, among which (E)-4-methoxy-5-(3-methoxybut-1-enyl)-6-methyl-2H-pyran-2-one is a new metabolite isolated from the Penicillium paxilli strain Ma(G)K. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000200003 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0102-695X2012000200003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0102-695X2011005000222 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Farmacognosia |
dc.source.none.fl_str_mv |
Revista Brasileira de Farmacognosia v.22 n.2 2012 reponame:Revista Brasileira de Farmacognosia (Online) instname:Sociedade Brasileira de Farmacognosia (SBFgnosia) instacron:SBFGNOSIA |
instname_str |
Sociedade Brasileira de Farmacognosia (SBFgnosia) |
instacron_str |
SBFGNOSIA |
institution |
SBFGNOSIA |
reponame_str |
Revista Brasileira de Farmacognosia (Online) |
collection |
Revista Brasileira de Farmacognosia (Online) |
repository.name.fl_str_mv |
Revista Brasileira de Farmacognosia (Online) - Sociedade Brasileira de Farmacognosia (SBFgnosia) |
repository.mail.fl_str_mv |
rbgnosia@ltf.ufpb.br |
_version_ |
1752122466612805632 |