Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400012 |
Resumo: | This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched. |
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Brazilian Journal of Microbiology |
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|
spelling |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler partsImmunomagnetic separationSalmonellaraw broiler partsThis study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.Sociedade Brasileira de Microbiologia2002-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400012Brazilian Journal of Microbiology v.33 n.4 2002reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822002000400012info:eu-repo/semantics/openAccessRibeiro,Aldemir ReginatoNascimento,Vladimir Pinheiro doCardoso,Martha OliveiraSantos,Luciana Ruschel dosRocha,Silvio Luís da Silveiraeng2014-04-28T00:00:00Zoai:scielo:S1517-83822002000400012Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-04-28T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
spellingShingle |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts Ribeiro,Aldemir Reginato Immunomagnetic separation Salmonella raw broiler parts |
title_short |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title_full |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title_fullStr |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title_full_unstemmed |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title_sort |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
author |
Ribeiro,Aldemir Reginato |
author_facet |
Ribeiro,Aldemir Reginato Nascimento,Vladimir Pinheiro do Cardoso,Martha Oliveira Santos,Luciana Ruschel dos Rocha,Silvio Luís da Silveira |
author_role |
author |
author2 |
Nascimento,Vladimir Pinheiro do Cardoso,Martha Oliveira Santos,Luciana Ruschel dos Rocha,Silvio Luís da Silveira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro,Aldemir Reginato Nascimento,Vladimir Pinheiro do Cardoso,Martha Oliveira Santos,Luciana Ruschel dos Rocha,Silvio Luís da Silveira |
dc.subject.por.fl_str_mv |
Immunomagnetic separation Salmonella raw broiler parts |
topic |
Immunomagnetic separation Salmonella raw broiler parts |
description |
This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched. |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400012 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000400012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822002000400012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.33 n.4 2002 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122199337074688 |