Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UFRGS |
Texto Completo: | http://hdl.handle.net/10183/19662 |
Resumo: | This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/ RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched. |
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Ribeiro, Aldemir ReginatoNascimento, Vladimir Pinheiro doCardoso, Martha OliveiraSantos, Luciana Ruschel dosRocha, Silvio Luis da Silveira2010-04-16T09:11:06Z20021517-8382http://hdl.handle.net/10183/19662000372078This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/ RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched.application/pdfporBrazilian journal of microbiology. São Paulo, SP. Vol. 33, n. 4 (out./dez. 2002), p. 339-341Salmonella : AvesImmunomagnetic separationSalmonellaRaw broiler partsUtilization of immunomagnetic separation for detection of Salmonella in raw broiler partsinfo:eu-repo/semantics/articleinfo:eu-repo/semantics/otherinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da UFRGSinstname:Universidade Federal do Rio Grande do Sul (UFRGS)instacron:UFRGSORIGINAL000372078.pdf000372078.pdfTexto completoapplication/pdf28096http://www.lume.ufrgs.br/bitstream/10183/19662/1/000372078.pdf61053900146bcba933e53aa003916673MD51TEXT000372078.pdf.txt000372078.pdf.txtExtracted Texttext/plain14909http://www.lume.ufrgs.br/bitstream/10183/19662/2/000372078.pdf.txt3479588ef957d55c12ba6c0cbe7c4012MD52THUMBNAIL000372078.pdf.jpg000372078.pdf.jpgGenerated Thumbnailimage/jpeg1753http://www.lume.ufrgs.br/bitstream/10183/19662/3/000372078.pdf.jpga8dc9cc6efe629d093a4959ad4d1fd5eMD5310183/196622018-10-11 08:58:49.802oai:www.lume.ufrgs.br:10183/19662Repositório de PublicaçõesPUBhttps://lume.ufrgs.br/oai/requestopendoar:2018-10-11T11:58:49Repositório Institucional da UFRGS - Universidade Federal do Rio Grande do Sul (UFRGS)false |
dc.title.pt_BR.fl_str_mv |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
spellingShingle |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts Ribeiro, Aldemir Reginato Salmonella : Aves Immunomagnetic separation Salmonella Raw broiler parts |
title_short |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title_full |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title_fullStr |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title_full_unstemmed |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
title_sort |
Utilization of immunomagnetic separation for detection of Salmonella in raw broiler parts |
author |
Ribeiro, Aldemir Reginato |
author_facet |
Ribeiro, Aldemir Reginato Nascimento, Vladimir Pinheiro do Cardoso, Martha Oliveira Santos, Luciana Ruschel dos Rocha, Silvio Luis da Silveira |
author_role |
author |
author2 |
Nascimento, Vladimir Pinheiro do Cardoso, Martha Oliveira Santos, Luciana Ruschel dos Rocha, Silvio Luis da Silveira |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Ribeiro, Aldemir Reginato Nascimento, Vladimir Pinheiro do Cardoso, Martha Oliveira Santos, Luciana Ruschel dos Rocha, Silvio Luis da Silveira |
dc.subject.por.fl_str_mv |
Salmonella : Aves |
topic |
Salmonella : Aves Immunomagnetic separation Salmonella Raw broiler parts |
dc.subject.eng.fl_str_mv |
Immunomagnetic separation Salmonella Raw broiler parts |
description |
This study was conducted aiming to compare the conventional microbiological method to detect Salmonella in broiler parts with the Immunomagnetic Separation method (IMS) followed by plate isolation and also the IMS associated with Rappaport-Vassiliadis broth (RV). The IMS was performed following a pre- enrichment step in buffered peptone water. Sixty-one samples (raw broiler parts) were tested and the results showed that the use of the IMS method alone allowed the isolation of Salmonella in 9 of the tested samples, while the association IMS/ RV detected the agent in 30 samples. The conventional microbiological method was able to isolate the agent in 25 opportunities. These results allowed to conclude that the IMS/RV association presented an increased sensitivity and permitted a better isolation of Salmonella. The conclusion was that other means of isolation, in particular those which do not interfere with the growth of bead bounded Salmonella, should be searched. |
publishDate |
2002 |
dc.date.issued.fl_str_mv |
2002 |
dc.date.accessioned.fl_str_mv |
2010-04-16T09:11:06Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/other |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://hdl.handle.net/10183/19662 |
dc.identifier.issn.pt_BR.fl_str_mv |
1517-8382 |
dc.identifier.nrb.pt_BR.fl_str_mv |
000372078 |
identifier_str_mv |
1517-8382 000372078 |
url |
http://hdl.handle.net/10183/19662 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.ispartof.pt_BR.fl_str_mv |
Brazilian journal of microbiology. São Paulo, SP. Vol. 33, n. 4 (out./dez. 2002), p. 339-341 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
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application/pdf |
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