Detection of rotavirus in dogs with diarrhea in Brazil

Detalhes bibliográficos
Autor(a) principal: Gabbay,Yvone B.
Data de Publicação: 2003
Outros Autores: Homem,Valéria S.F., Munford,Veridiana, Alves,Antonia S., Mascarenhas,Joana D.P., Linhares,Alexandre C., Rácz,Maria Lúcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100016
Resumo: Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrheic dog. Virus particles with morphology typical of rotavirus were visualized by direct electron microscopy. This sample was subsequently tested for the four main human serotypes (G1-G4), by ELISA with monoclonal antibodies. G genotyping was attempted by RT-PCR using G1-G6 and G8-G11 primers but no positive results could be yielded. Also using RT-PCR it was possible to characterize this canine strain as belonging to P[ 3] genotype. This is the first canine rotavirus detected in Brazil.
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spelling Detection of rotavirus in dogs with diarrhea in Brazilrotavirusdiarrheic doggenotype Ggenotype PBelém- BrazilRotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrheic dog. Virus particles with morphology typical of rotavirus were visualized by direct electron microscopy. This sample was subsequently tested for the four main human serotypes (G1-G4), by ELISA with monoclonal antibodies. G genotyping was attempted by RT-PCR using G1-G6 and G8-G11 primers but no positive results could be yielded. Also using RT-PCR it was possible to characterize this canine strain as belonging to P[ 3] genotype. This is the first canine rotavirus detected in Brazil.Sociedade Brasileira de Microbiologia2003-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100016Brazilian Journal of Microbiology v.34 n.1 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000100016info:eu-repo/semantics/openAccessGabbay,Yvone B.Homem,Valéria S.F.Munford,VeridianaAlves,Antonia S.Mascarenhas,Joana D.P.Linhares,Alexandre C.Rácz,Maria Lúciaeng2003-06-30T00:00:00Zoai:scielo:S1517-83822003000100016Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2003-06-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Detection of rotavirus in dogs with diarrhea in Brazil
title Detection of rotavirus in dogs with diarrhea in Brazil
spellingShingle Detection of rotavirus in dogs with diarrhea in Brazil
Gabbay,Yvone B.
rotavirus
diarrheic dog
genotype G
genotype P
Belém- Brazil
title_short Detection of rotavirus in dogs with diarrhea in Brazil
title_full Detection of rotavirus in dogs with diarrhea in Brazil
title_fullStr Detection of rotavirus in dogs with diarrhea in Brazil
title_full_unstemmed Detection of rotavirus in dogs with diarrhea in Brazil
title_sort Detection of rotavirus in dogs with diarrhea in Brazil
author Gabbay,Yvone B.
author_facet Gabbay,Yvone B.
Homem,Valéria S.F.
Munford,Veridiana
Alves,Antonia S.
Mascarenhas,Joana D.P.
Linhares,Alexandre C.
Rácz,Maria Lúcia
author_role author
author2 Homem,Valéria S.F.
Munford,Veridiana
Alves,Antonia S.
Mascarenhas,Joana D.P.
Linhares,Alexandre C.
Rácz,Maria Lúcia
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gabbay,Yvone B.
Homem,Valéria S.F.
Munford,Veridiana
Alves,Antonia S.
Mascarenhas,Joana D.P.
Linhares,Alexandre C.
Rácz,Maria Lúcia
dc.subject.por.fl_str_mv rotavirus
diarrheic dog
genotype G
genotype P
Belém- Brazil
topic rotavirus
diarrheic dog
genotype G
genotype P
Belém- Brazil
description Rotavirus was detected by the enzyme-linked immunosorbent assay (ELISA) in the faeces of a diarrheic dog. Virus particles with morphology typical of rotavirus were visualized by direct electron microscopy. This sample was subsequently tested for the four main human serotypes (G1-G4), by ELISA with monoclonal antibodies. G genotyping was attempted by RT-PCR using G1-G6 and G8-G11 primers but no positive results could be yielded. Also using RT-PCR it was possible to characterize this canine strain as belonging to P[ 3] genotype. This is the first canine rotavirus detected in Brazil.
publishDate 2003
dc.date.none.fl_str_mv 2003-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822003000100016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.34 n.1 2003
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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