Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil

Detalhes bibliográficos
Autor(a) principal: Angelini,Michelle
Data de Publicação: 2009
Outros Autores: Stehling,Eliana Guedes, Moretti,Maria Luiza, Silveira,Wanderley Dias da
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034
Resumo: Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated (i) the existence of just a few strain clusters for both species, but with genotype variability with either a high speed of genetic change or constant introduction of several genotypes, considering the intense migration to these two metropolitan areas, and (ii) the prevalence of specific genotypes in each geographical area, which suggests the successful adaptation of some genotypes to the local environmental conditions. Our results indicate the need of more efficacious sanitary barriers to prevent Shigella spp. outbreaks and epidemics.
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spelling Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast BrazilShigella sonneiShigella flexneriEpidemiologyCharacterizationPFGEShigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated (i) the existence of just a few strain clusters for both species, but with genotype variability with either a high speed of genetic change or constant introduction of several genotypes, considering the intense migration to these two metropolitan areas, and (ii) the prevalence of specific genotypes in each geographical area, which suggests the successful adaptation of some genotypes to the local environmental conditions. Our results indicate the need of more efficacious sanitary barriers to prevent Shigella spp. outbreaks and epidemics.Sociedade Brasileira de Microbiologia2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034Brazilian Journal of Microbiology v.40 n.3 2009reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822009000300034info:eu-repo/semantics/openAccessAngelini,MichelleStehling,Eliana GuedesMoretti,Maria LuizaSilveira,Wanderley Dias daeng2009-08-18T00:00:00Zoai:scielo:S1517-83822009000300034Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2009-08-18T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil
title Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil
spellingShingle Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil
Angelini,Michelle
Shigella sonnei
Shigella flexneri
Epidemiology
Characterization
PFGE
title_short Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil
title_full Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil
title_fullStr Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil
title_full_unstemmed Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil
title_sort Molecular epidemiology of Shigella spp strains isolated in two different metropolitam areas of southeast Brazil
author Angelini,Michelle
author_facet Angelini,Michelle
Stehling,Eliana Guedes
Moretti,Maria Luiza
Silveira,Wanderley Dias da
author_role author
author2 Stehling,Eliana Guedes
Moretti,Maria Luiza
Silveira,Wanderley Dias da
author2_role author
author
author
dc.contributor.author.fl_str_mv Angelini,Michelle
Stehling,Eliana Guedes
Moretti,Maria Luiza
Silveira,Wanderley Dias da
dc.subject.por.fl_str_mv Shigella sonnei
Shigella flexneri
Epidemiology
Characterization
PFGE
topic Shigella sonnei
Shigella flexneri
Epidemiology
Characterization
PFGE
description Shigella spp., the human pathogen responsible for shigellosis, is highly infectious even at low levels. The incidence rate of shigellosis varies with geographical distribution, location human development index, and age groups, being higher among children aged under 5 years. In Brazil, a few works indicate that shigellosis cases are underestimated, with S. flexneri and S. sonnei strains being the major agents responsible for the shigellosis cases. The present study used pulsed field gel electrophoresis (PFGE) to investigate the molecular epidemiology of 119 strains of S. sonnei and S. flexneri isolated from shigellosis cases that occurred in the metropolitan areas of Ribeirão Preto and Campinas Cities, São Paulo Sate, southeast Brazil. The results indicated (i) the existence of just a few strain clusters for both species, but with genotype variability with either a high speed of genetic change or constant introduction of several genotypes, considering the intense migration to these two metropolitan areas, and (ii) the prevalence of specific genotypes in each geographical area, which suggests the successful adaptation of some genotypes to the local environmental conditions. Our results indicate the need of more efficacious sanitary barriers to prevent Shigella spp. outbreaks and epidemics.
publishDate 2009
dc.date.none.fl_str_mv 2009-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300034
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822009000300034
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.40 n.3 2009
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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