Detection of HTLV-IIa in blood donors in an urban area of the Amazon Region of Brazil (Belém, PA)

• Main Author: Ishak,R.
• Publication Date: 1998
• Other Authors: Ishak,M.O.G., Azevedo,V.N., Santos,D.E.M., Vallinoto,A.C.R., Crescente,J.A., Hall,W.W.
• Format: Article
• Language: eng
• Source: Revista da Sociedade Brasileira de Medicina Tropical
• Download full: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86821998000200005
• topic: HTLV-II; Retrovirus; Blood donors; Blood

The human lymphotropic viruses type I (HTLV-I) and type II (HTLV-II) are members of a group of mammalian retroviruses with similar biological properties, and blood transfusion is an important route of transmission. HTLV-I is endemic in a number of different geographical areas and is associated with several clinical disorders. HTLV-II is endemic in several Indian groups of the Americas and intravenous drug abusers in North and South America, Europe and Southeast Asia. During the year of 1995, all blood donors tested positive to HTLV-I/II in the State Blood Bank (HEMOPA), were directed to a physician and to the Virus Laboratory at the Universidade Federal do Pará for counselling and laboratory diagnosis confirmation. Thirty-five sera were tested by an enzyme immune assay, and a Western blot that discriminates HTLV-I and HTLV-II infection. Two HTLV-II positive samples were submitted to PCR analysis of pX and env genomic region, and confirmed to be of subtype IIa. This is the first detection in Belém of the presence of HTLV-IIa infection among blood donors. This result emphasizes that HTLV-II is also present in urban areas of the Amazon region of Brazil and highlights the need to include screening tests that are capable to detect antibodies for both types of HTLV.