METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION

Detalhes bibliográficos
Autor(a) principal: de Pádua, Santiago Benites
Data de Publicação: 2011
Outros Autores: Ishikawa, Márcia Mayumi
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Medicine
Texto Completo: https://rbmv.org/BJVM/article/view/803
Resumo: ABSTRACT. Pádua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificação de basófilos sanguíneos em surubim híbrido: contribuição metodológica. Revista Brasileira de Medicina Veterinária, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: santiagopadua@live.com Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.
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spelling METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTIONMETACROMASIA PARA IDENTIFICAÇÃO DE BASÓFILOS SANGUÍNEOS EM SURUBIM HÍBRIDO: CONTRIBUIÇÃO METODOLÓGICAMarcação de basófilosazul de toluidinacitoquímicahematologia de peixesBasophils markingtoluidine bluecytochemistryfish hematologyABSTRACT. Pádua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificação de basófilos sanguíneos em surubim híbrido: contribuição metodológica. Revista Brasileira de Medicina Veterinária, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: santiagopadua@live.com Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.O presente estudo avaliou diferentes protocolos de fixação e hidrólise das extensões sanguíneas de surubim híbrido para reação de metacromasia e marcação de basófilos sanguíneos. Para isso, metanol, álcool-ácido e vapor de formol foram testados como fixadores. Para hidrólise das extensões sanguíneas foi avaliado HCl, ácido cítrico e soluções de 2-Mercaptoetanol + Uréia + NaCl. Após estes procedimentos, as extensões foram submetidas à coloração com azul de toluidina (0,025%) diluída em tampão de McIlvaine (pH 4). Diferentes protocolos para fixação e hidrólise das extensões sanguíneas destinadas à reação de metacromasia influenciam na qualidade da reação. A hidrólise com soluções de 2-Mercaptoetanol + Uréia + NaCl proporcionaram resultados negativos quando utilizado metanol e álcool-ácido como fixadores. A fixação com álcool-ácido associada à hidrólise com ácido cítrico proporciona reações com melhor qualidade.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2011-10-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/803Brazilian Journal of Veterinary Medicine; Vol. 33 No. 3 (2011); 147-150Revista Brasileira de Medicina Veterinária; v. 33 n. 3 (2011); 147-1502527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/803/659de Pádua, Santiago BenitesIshikawa, Márcia Mayumiinfo:eu-repo/semantics/openAccess2020-12-23T17:28:23Zoai:ojs.rbmv.org:article/803Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:28:23Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false
dc.title.none.fl_str_mv METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
METACROMASIA PARA IDENTIFICAÇÃO DE BASÓFILOS SANGUÍNEOS EM SURUBIM HÍBRIDO: CONTRIBUIÇÃO METODOLÓGICA
title METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
spellingShingle METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
de Pádua, Santiago Benites
Marcação de basófilos
azul de toluidina
citoquímica
hematologia de peixes
Basophils marking
toluidine blue
cytochemistry
fish hematology
title_short METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
title_full METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
title_fullStr METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
title_full_unstemmed METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
title_sort METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
author de Pádua, Santiago Benites
author_facet de Pádua, Santiago Benites
Ishikawa, Márcia Mayumi
author_role author
author2 Ishikawa, Márcia Mayumi
author2_role author
dc.contributor.author.fl_str_mv de Pádua, Santiago Benites
Ishikawa, Márcia Mayumi
dc.subject.por.fl_str_mv Marcação de basófilos
azul de toluidina
citoquímica
hematologia de peixes
Basophils marking
toluidine blue
cytochemistry
fish hematology
topic Marcação de basófilos
azul de toluidina
citoquímica
hematologia de peixes
Basophils marking
toluidine blue
cytochemistry
fish hematology
description ABSTRACT. Pádua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificação de basófilos sanguíneos em surubim híbrido: contribuição metodológica. Revista Brasileira de Medicina Veterinária, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: santiagopadua@live.com Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.
publishDate 2011
dc.date.none.fl_str_mv 2011-10-31
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
peer reviewed
Avaliado pelos pares
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://rbmv.org/BJVM/article/view/803
url https://rbmv.org/BJVM/article/view/803
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://rbmv.org/BJVM/article/view/803/659
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
publisher.none.fl_str_mv Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Medicine; Vol. 33 No. 3 (2011); 147-150
Revista Brasileira de Medicina Veterinária; v. 33 n. 3 (2011); 147-150
2527-2179
0100-2430
reponame:Brazilian Journal of Veterinary Medicine
instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron:SBMV
instname_str Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
instacron_str SBMV
institution SBMV
reponame_str Brazilian Journal of Veterinary Medicine
collection Brazilian Journal of Veterinary Medicine
repository.name.fl_str_mv Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)
repository.mail.fl_str_mv contato.rbmv@gmail.com
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