Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution

Detalhes bibliográficos
Autor(a) principal: De Pádua, Santiago Benites [UNESP]
Data de Publicação: 2011
Outros Autores: Ishikawa, Márcia Mayumi
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://hdl.handle.net/11449/227576
Resumo: Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.
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spelling Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contributionMetacromasia para identificação de basófilos sanguíneos em surubim híbrido: Contribuição metodológicaBasophils markingCytochemistryFish hematologyToluidine blueDifferent protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.Centro de Aquicultura da Unesp (CAUNESP), Rod. Paulo Donato Castellane, Jaboticabal, SP 14884-900Embrapa Agropecuária Oeste, BR 163, Caixa Postal 661, Dourados, MS 79804-970Centro de Aquicultura da Unesp (CAUNESP), Rod. Paulo Donato Castellane, Jaboticabal, SP 14884-900Universidade Estadual Paulista (UNESP)Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)De Pádua, Santiago Benites [UNESP]Ishikawa, Márcia Mayumi2022-04-29T07:13:57Z2022-04-29T07:13:57Z2011-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article147-150Revista Brasileira de Medicina Veterinaria, v. 33, n. 3, p. 147-150, 2011.2527-21790100-2430http://hdl.handle.net/11449/2275762-s2.0-84894299439Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporRevista Brasileira de Medicina Veterinariainfo:eu-repo/semantics/openAccess2022-04-29T07:13:57Zoai:repositorio.unesp.br:11449/227576Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:13:57Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution
Metacromasia para identificação de basófilos sanguíneos em surubim híbrido: Contribuição metodológica
title Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution
spellingShingle Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution
De Pádua, Santiago Benites [UNESP]
Basophils marking
Cytochemistry
Fish hematology
Toluidine blue
title_short Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution
title_full Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution
title_fullStr Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution
title_full_unstemmed Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution
title_sort Metachromasia for blood basophils identification in hybrid surubim catfish: Methodological contribution
author De Pádua, Santiago Benites [UNESP]
author_facet De Pádua, Santiago Benites [UNESP]
Ishikawa, Márcia Mayumi
author_role author
author2 Ishikawa, Márcia Mayumi
author2_role author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)
dc.contributor.author.fl_str_mv De Pádua, Santiago Benites [UNESP]
Ishikawa, Márcia Mayumi
dc.subject.por.fl_str_mv Basophils marking
Cytochemistry
Fish hematology
Toluidine blue
topic Basophils marking
Cytochemistry
Fish hematology
Toluidine blue
description Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
2022-04-29T07:13:57Z
2022-04-29T07:13:57Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Revista Brasileira de Medicina Veterinaria, v. 33, n. 3, p. 147-150, 2011.
2527-2179
0100-2430
http://hdl.handle.net/11449/227576
2-s2.0-84894299439
identifier_str_mv Revista Brasileira de Medicina Veterinaria, v. 33, n. 3, p. 147-150, 2011.
2527-2179
0100-2430
2-s2.0-84894299439
url http://hdl.handle.net/11449/227576
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv Revista Brasileira de Medicina Veterinaria
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 147-150
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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