METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/803 |
Resumo: | ABSTRACT. Pádua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificação de basófilos sanguíneos em surubim híbrido: contribuição metodológica. Revista Brasileira de Medicina Veterinária, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: santiagopadua@live.com Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason. |
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Brazilian Journal of Veterinary Medicine |
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METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTIONMETACROMASIA PARA IDENTIFICAÇÃO DE BASÓFILOS SANGUÍNEOS EM SURUBIM HÍBRIDO: CONTRIBUIÇÃO METODOLÓGICAMarcação de basófilosazul de toluidinacitoquímicahematologia de peixesBasophils markingtoluidine bluecytochemistryfish hematologyABSTRACT. Pádua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificação de basófilos sanguíneos em surubim híbrido: contribuição metodológica. Revista Brasileira de Medicina Veterinária, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: santiagopadua@live.com Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason.O presente estudo avaliou diferentes protocolos de fixação e hidrólise das extensões sanguíneas de surubim híbrido para reação de metacromasia e marcação de basófilos sanguíneos. Para isso, metanol, álcool-ácido e vapor de formol foram testados como fixadores. Para hidrólise das extensões sanguíneas foi avaliado HCl, ácido cítrico e soluções de 2-Mercaptoetanol + Uréia + NaCl. Após estes procedimentos, as extensões foram submetidas à coloração com azul de toluidina (0,025%) diluída em tampão de McIlvaine (pH 4). Diferentes protocolos para fixação e hidrólise das extensões sanguíneas destinadas à reação de metacromasia influenciam na qualidade da reação. A hidrólise com soluções de 2-Mercaptoetanol + Uréia + NaCl proporcionaram resultados negativos quando utilizado metanol e álcool-ácido como fixadores. A fixação com álcool-ácido associada à hidrólise com ácido cítrico proporciona reações com melhor qualidade.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2011-10-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/803Brazilian Journal of Veterinary Medicine; Vol. 33 No. 3 (2011); 147-150Revista Brasileira de Medicina Veterinária; v. 33 n. 3 (2011); 147-1502527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/803/659de Pádua, Santiago BenitesIshikawa, Márcia Mayumiinfo:eu-repo/semantics/openAccess2020-12-23T17:28:23Zoai:ojs.rbmv.org:article/803Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:28:23Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION METACROMASIA PARA IDENTIFICAÇÃO DE BASÓFILOS SANGUÍNEOS EM SURUBIM HÍBRIDO: CONTRIBUIÇÃO METODOLÓGICA |
title |
METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION |
spellingShingle |
METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION de Pádua, Santiago Benites Marcação de basófilos azul de toluidina citoquímica hematologia de peixes Basophils marking toluidine blue cytochemistry fish hematology |
title_short |
METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION |
title_full |
METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION |
title_fullStr |
METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION |
title_full_unstemmed |
METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION |
title_sort |
METACHROMASIA FOR BLOOD BASOPHILS IDENTIFICATION IN HYBRID SURUBIM CATFISH: METHODOLOGICAL CONTRIBUTION |
author |
de Pádua, Santiago Benites |
author_facet |
de Pádua, Santiago Benites Ishikawa, Márcia Mayumi |
author_role |
author |
author2 |
Ishikawa, Márcia Mayumi |
author2_role |
author |
dc.contributor.author.fl_str_mv |
de Pádua, Santiago Benites Ishikawa, Márcia Mayumi |
dc.subject.por.fl_str_mv |
Marcação de basófilos azul de toluidina citoquímica hematologia de peixes Basophils marking toluidine blue cytochemistry fish hematology |
topic |
Marcação de basófilos azul de toluidina citoquímica hematologia de peixes Basophils marking toluidine blue cytochemistry fish hematology |
description |
ABSTRACT. Pádua S.B. & Ishikawa M.M. [Metachromasia for blood basophils identification in hybrid surubim catfish: methodological contribution]. Metacromasia para identificação de basófilos sanguíneos em surubim híbrido: contribuição metodológica. Revista Brasileira de Medicina Veterinária, 33(3):147-150, 2011. Centro de Aquicultura da Unesp/Jaboticabal Rod. Paulo Donato Castellane, s/n Bairro Rural, Jaboticabal, 14884-900, SP. Brasil. E-mail: santiagopadua@live.com Different protocols of fixation and hydrolysis of blood smears of hybrid surubim catfish for metachromasia to marking blood basophils were evaluated. For this reason, methanol, acid-alcohol and formalin vapor were tested as fixatives. For hydrolysis of blood smears, HCl, citric acid and 2-Mercaptoethanol + Urea + NaCl solutions was evaluated. After procedures, the blood smears was stained with toluidine blue (0.025%) diluted in McIlvaine buffer (pH 4). The different protocols for fixation and hydrolysis of blood smears for metachromasia influenced the quality of the reaction. Hydrolysis with 2-Mercaptoethanol + Urea + NaCl solutions provided negative results when using methanol and acid-alcohol as fixatives. The fixation with acid-alcohol associated with citric acid hydrolysis provides highest quality reason. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-10-31 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/803 |
url |
https://rbmv.org/BJVM/article/view/803 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/803/659 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 33 No. 3 (2011); 147-150 Revista Brasileira de Medicina Veterinária; v. 33 n. 3 (2011); 147-150 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313109893414912 |