Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Medicine |
Texto Completo: | https://rbmv.org/BJVM/article/view/625 |
Resumo: | ABSTRACT. Resende H.L., Oliveira J.P., Ecker M.K.C., Guasti P.N. & Papa F.O. & Jacob J.C.F. [Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours]. Criopreservação de Sêmen Equino previamente refrigerado com e sem plasma seminal por 12 horas. Revista Brasileira de Medicina Veterinária, 35(4):306-310, 2013. Programa de Pós-Graduação em Medicina Veteriná- ria, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Univerversidade Estadual Paulista Júlio de Mesquita Filho, Unesp/CampusBotucatu, RubiãoJunior, Botucatu, SP 18618-000, Brasil. E-mail: helene_resende@hotmail.com In the equine species, the use of frozen semen has limiting factors as the need for skilled labor, the high cost of equipment, as well as an appropriate place on the farms to the handling of semen. In order to evaluate the effect of seminal plasma on cooling of equine semen for 12 hours before cryopreservation, this study was conducted with five stallions of Mangalarga Marchador breed, aged between 4 and 8 years old. For this experiment, 15 semen collections were performed, each ejaculate was divided into three groups and each group was divided into two subgroups, using different media extenders (Botu-Semen® and Botu-Turbo®). In Group I (control), the semen was frozen immediately after collection; in Group II, the seminal plasma was maintained during the cooling and in Group III, the seminal plasma was removed before cooling. After thawing, the sperm parameters were analyzed using CASA and membrane integrity was evaluated by the fluorescent probes carboxyfluorescein diacetate and propidium iodide. Statistical analysis was performed by ANOVA and Tukey’s test. There was no significant differences (P>0.05) between the semen samples with and without seminal plasma prior to freezing for all sperm parameters evaluated, total motility (50.5 vs 50.6), progressive motility (21.3 vs 24.1) and plasma membrane integrity (26.2 vs 28). The analyzed data showed that the cooling of equine semen for 12 hours before cryopreservation is an effective method that enables a better use of the ejaculate, regardless of maintenance or removal of seminal plasma during the semen storage. |
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Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hoursCRIOPRESERVAÇÃO DE SÊMEN EQUINO PREVIAMENTE REFRIGERADO COM E SEM PLASMA SEMINAL POR 12 HORASGaranhãocongelação de sêmenfrações do sêmenextensor de sêmenStallionfreezingfractions of semenextenderABSTRACT. Resende H.L., Oliveira J.P., Ecker M.K.C., Guasti P.N. & Papa F.O. & Jacob J.C.F. [Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours]. Criopreservação de Sêmen Equino previamente refrigerado com e sem plasma seminal por 12 horas. Revista Brasileira de Medicina Veterinária, 35(4):306-310, 2013. Programa de Pós-Graduação em Medicina Veteriná- ria, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Univerversidade Estadual Paulista Júlio de Mesquita Filho, Unesp/CampusBotucatu, RubiãoJunior, Botucatu, SP 18618-000, Brasil. E-mail: helene_resende@hotmail.com In the equine species, the use of frozen semen has limiting factors as the need for skilled labor, the high cost of equipment, as well as an appropriate place on the farms to the handling of semen. In order to evaluate the effect of seminal plasma on cooling of equine semen for 12 hours before cryopreservation, this study was conducted with five stallions of Mangalarga Marchador breed, aged between 4 and 8 years old. For this experiment, 15 semen collections were performed, each ejaculate was divided into three groups and each group was divided into two subgroups, using different media extenders (Botu-Semen® and Botu-Turbo®). In Group I (control), the semen was frozen immediately after collection; in Group II, the seminal plasma was maintained during the cooling and in Group III, the seminal plasma was removed before cooling. After thawing, the sperm parameters were analyzed using CASA and membrane integrity was evaluated by the fluorescent probes carboxyfluorescein diacetate and propidium iodide. Statistical analysis was performed by ANOVA and Tukey’s test. There was no significant differences (P>0.05) between the semen samples with and without seminal plasma prior to freezing for all sperm parameters evaluated, total motility (50.5 vs 50.6), progressive motility (21.3 vs 24.1) and plasma membrane integrity (26.2 vs 28). The analyzed data showed that the cooling of equine semen for 12 hours before cryopreservation is an effective method that enables a better use of the ejaculate, regardless of maintenance or removal of seminal plasma during the semen storage.Na espécie equina a utilização do sê- men congelado tem fatores limitantes como a necessidade de mão de obra especializada, o alto custo dos equipamentos, assim como locais apropriados nos haras para manipulação do sêmen. Com o objetivo de avaliar o efeito do plasma seminal na refrigeração do sêmen equino por 12 horas antes da criopreservação, o estudo foi conduzido com cinco garanhões da raça Mangalarga Marchador, com idade entre 4 e 8 anos. Foram realizadas 15 colheitas de sêmen, sendo que cada ejaculado foi divido em três grupos, e cada grupo foi dividido em dois subgrupos, utilizando diferentes meios extensores (Botu-Sêmen® e Botu-Turbo®). A amostra do Grupo I (controle) foi congelada logo após a coleta, o sêmen do Grupo II teve o plasma seminal mantido durante a refrigeração e o sêmen do Grupo III teve o plasma seminal retirado para refrigerar. Após o descongelamento, os parâmetros espermáticos de motilidade foram analisados utilizando CASA e a integridade da membrana plasmática foi avaliada por meio de sondas fluorescentes de diacetato de carboxifluoresceína e iodeto de propídio. A aná- lise estatística foi realizada através da ANOVA e teste de Tukey. Não houve diferença significativa (P>0,05) entre o sêmen, previamente a congelação, refrigerado com e sem plasma seminal para todos os parâmetros espermáticos avaliados, sendo estes motilidade total (50,5 vs 50,6), motilidade progressiva (21,3 vs 24,1) e integridade de membrana plasmática (26,2 vs 28). Os dados analisados indicaram que a refrigeração do sêmen equino por 12 horas antes da criopreservação é um método eficaz, que possibilita um melhor aproveitamento do ejaculado, independentemente da manutenção ou remoção do plasma seminal durante a refrigeração.Sociedade de Medicina Veterinária do Estado do Rio de Janeiro.2013-12-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionpeer reviewedAvaliado pelos paresapplication/pdfhttps://rbmv.org/BJVM/article/view/625Brazilian Journal of Veterinary Medicine; Vol. 35 No. 4 (2013); 306-310Revista Brasileira de Medicina Veterinária; v. 35 n. 4 (2013); 306-3102527-21790100-2430reponame:Brazilian Journal of Veterinary Medicineinstname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)instacron:SBMVporhttps://rbmv.org/BJVM/article/view/625/489de Resende, Hélène LacerdaOliveira, Jhonnatha PauloEcker, Marcely Karoline ConceiçãoGuasti, Priscilla NascimentoPapa, Frederico OzanamJacob, Júlio César Ferrazinfo:eu-repo/semantics/openAccess2020-12-23T17:30:52Zoai:ojs.rbmv.org:article/625Revistahttps://rbmv.org/BJVMONGhttps://rbmv.org/BJVM/oaicontato.rbmv@gmail.com2527-21790100-2430opendoar:2020-12-23T17:30:52Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ)false |
dc.title.none.fl_str_mv |
Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours CRIOPRESERVAÇÃO DE SÊMEN EQUINO PREVIAMENTE REFRIGERADO COM E SEM PLASMA SEMINAL POR 12 HORAS |
title |
Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours |
spellingShingle |
Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours de Resende, Hélène Lacerda Garanhão congelação de sêmen frações do sêmen extensor de sêmen Stallion freezing fractions of semen extender |
title_short |
Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours |
title_full |
Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours |
title_fullStr |
Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours |
title_full_unstemmed |
Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours |
title_sort |
Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours |
author |
de Resende, Hélène Lacerda |
author_facet |
de Resende, Hélène Lacerda Oliveira, Jhonnatha Paulo Ecker, Marcely Karoline Conceição Guasti, Priscilla Nascimento Papa, Frederico Ozanam Jacob, Júlio César Ferraz |
author_role |
author |
author2 |
Oliveira, Jhonnatha Paulo Ecker, Marcely Karoline Conceição Guasti, Priscilla Nascimento Papa, Frederico Ozanam Jacob, Júlio César Ferraz |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
de Resende, Hélène Lacerda Oliveira, Jhonnatha Paulo Ecker, Marcely Karoline Conceição Guasti, Priscilla Nascimento Papa, Frederico Ozanam Jacob, Júlio César Ferraz |
dc.subject.por.fl_str_mv |
Garanhão congelação de sêmen frações do sêmen extensor de sêmen Stallion freezing fractions of semen extender |
topic |
Garanhão congelação de sêmen frações do sêmen extensor de sêmen Stallion freezing fractions of semen extender |
description |
ABSTRACT. Resende H.L., Oliveira J.P., Ecker M.K.C., Guasti P.N. & Papa F.O. & Jacob J.C.F. [Cryopreservation of Equine Semen previously cooled with and without seminal plasma for 12 hours]. Criopreservação de Sêmen Equino previamente refrigerado com e sem plasma seminal por 12 horas. Revista Brasileira de Medicina Veterinária, 35(4):306-310, 2013. Programa de Pós-Graduação em Medicina Veteriná- ria, Faculdade de Medicina Veterinária e Zootecnia (FMVZ), Univerversidade Estadual Paulista Júlio de Mesquita Filho, Unesp/CampusBotucatu, RubiãoJunior, Botucatu, SP 18618-000, Brasil. E-mail: helene_resende@hotmail.com In the equine species, the use of frozen semen has limiting factors as the need for skilled labor, the high cost of equipment, as well as an appropriate place on the farms to the handling of semen. In order to evaluate the effect of seminal plasma on cooling of equine semen for 12 hours before cryopreservation, this study was conducted with five stallions of Mangalarga Marchador breed, aged between 4 and 8 years old. For this experiment, 15 semen collections were performed, each ejaculate was divided into three groups and each group was divided into two subgroups, using different media extenders (Botu-Semen® and Botu-Turbo®). In Group I (control), the semen was frozen immediately after collection; in Group II, the seminal plasma was maintained during the cooling and in Group III, the seminal plasma was removed before cooling. After thawing, the sperm parameters were analyzed using CASA and membrane integrity was evaluated by the fluorescent probes carboxyfluorescein diacetate and propidium iodide. Statistical analysis was performed by ANOVA and Tukey’s test. There was no significant differences (P>0.05) between the semen samples with and without seminal plasma prior to freezing for all sperm parameters evaluated, total motility (50.5 vs 50.6), progressive motility (21.3 vs 24.1) and plasma membrane integrity (26.2 vs 28). The analyzed data showed that the cooling of equine semen for 12 hours before cryopreservation is an effective method that enables a better use of the ejaculate, regardless of maintenance or removal of seminal plasma during the semen storage. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-15 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion peer reviewed Avaliado pelos pares |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://rbmv.org/BJVM/article/view/625 |
url |
https://rbmv.org/BJVM/article/view/625 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://rbmv.org/BJVM/article/view/625/489 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
publisher.none.fl_str_mv |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Medicine; Vol. 35 No. 4 (2013); 306-310 Revista Brasileira de Medicina Veterinária; v. 35 n. 4 (2013); 306-310 2527-2179 0100-2430 reponame:Brazilian Journal of Veterinary Medicine instname:Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) instacron:SBMV |
instname_str |
Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
instacron_str |
SBMV |
institution |
SBMV |
reponame_str |
Brazilian Journal of Veterinary Medicine |
collection |
Brazilian Journal of Veterinary Medicine |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Medicine - Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ) |
repository.mail.fl_str_mv |
contato.rbmv@gmail.com |
_version_ |
1798313108794507264 |